PMID- 31523993
OWN - NLM
STAT- MEDLINE
DCOM- 20200204
LR  - 20221207
IS  - 2169-141X (Electronic)
IS  - 2169-1401 (Linking)
VI  - 47
IP  - 1
DP  - 2019 Dec
TI  - Matrine derivate MASM protects murine MC3T3-E1 osteoblastic cells against 
      dexamethasone-induced apoptosis via the regulation of USP14/p53.
PG  - 3720-3728
LID - 10.1080/21691401.2019.1664563 [doi]
AB  - Matrine derivative MASM (M19) is a quinolizine alkaloid with diverse 
      pharmacological effects including preventing postmenopausal osteoporosis. In the 
      current study, we observed that pretreatment with M19 inhibited cell apoptosis of 
      murine osteoblastic MC3T3-E1 and primary osteoblasts induced by 1 muM 
      dexamethasone in a dose-dependent manner. Our study also showed that pretreatment 
      with M19 significantly prevented the upregulation of p53 that is caused by 
      dexamethasone but had no effect on the p53 mRNA expression levels. Further 
      immunoprecipitation (IP) experiments showed that M19 treatment increases the 
      ubiquitination of p53 in dexamethasone-treated MC3T3-E1 cells. The expression of 
      USP14, a deubiquitinating enzyme, increased with dexamethasone and decreased with 
      M19 pretreatment. Co-IP experiments demonstrated the interaction between USP14 
      and p53, and the induced effect of a USP14 inhibitor (IU1) on p53 ubiquitination, 
      which indicated that USP14 is a potential deubiquitinase for p53. Furthermore, 
      pretreatment with IU1 or a p53 inhibitor (PFT-alpha) partially blocked 
      dexamethasone-induced apoptosis of MC3T3-E1 cells. The overexpression of USP14 or 
      p53 reversed the antiapoptotic effect of M19 in dexamethasone-treated MC3T3-E1 
      cells. In addition, PFT-alpha treatment remarkably blocked the effects of USP14 
      overexpression. In summary, our findings suggest that M19 exerts protective 
      effects on dexamethasone-treated MC3T3-E1 cells by regulating USP14/p53.
FAU - Zhou, Panyu
AU  - Zhou P
AD  - Department of Emergency, Changhai Hospital, Naval Military Medical University , 
      Shanghai , China.
AD  - Department of Orthopedics, Changhai Hospital, Naval Military Medical University , 
      Shanghai , China.
FAU - Xia, Demeng
AU  - Xia D
AD  - Department of Emergency, Changhai Hospital, Naval Military Medical University , 
      Shanghai , China.
FAU - Wang, Yang
AU  - Wang Y
AD  - Department of Emergency, Changhai Hospital, Naval Military Medical University , 
      Shanghai , China.
AD  - Department of Orthopedics, Changhai Hospital, Naval Military Medical University , 
      Shanghai , China.
FAU - Lv, Hongdi
AU  - Lv H
AD  - Department of Logistics, The Nine Nine Zero Hospital of the People's Liberation 
      Army , Henan , China.
FAU - Wang, Ziqin
AU  - Wang Z
AD  - Department of Nutrition, Armed Police Henan Corps Hospital , Henan , China.
FAU - Xing, Malcolm
AU  - Xing M
AD  - College of Mechanical Engineering, University of Manitoba , Winnipeg , Canada.
FAU - Zhao, Qingjie
AU  - Zhao Q
AD  - Department of Organic Chemistry, School of Pharmcy, Naval Military Medical 
      University , Shanghai , China.
FAU - Xu, Shuogui
AU  - Xu S
AD  - Department of Emergency, Changhai Hospital, Naval Military Medical University , 
      Shanghai , China.
AD  - Department of Orthopedics, Changhai Hospital, Naval Military Medical University , 
      Shanghai , China.
LA  - eng
PT  - Journal Article
PL  - England
TA  - Artif Cells Nanomed Biotechnol
JT  - Artificial cells, nanomedicine, and biotechnology
JID - 101594777
RN  - 0 (Alkaloids)
RN  - 0 (Quinolizines)
RN  - 0 (Tumor Suppressor Protein p53)
RN  - 0 (Usp14 protein, mouse)
RN  - 7S5I7G3JQL (Dexamethasone)
RN  - EC 3.4.19.12 (Ubiquitin Thiolesterase)
RN  - 0 (Matrines)
SB  - IM
MH  - 3T3 Cells
MH  - Alkaloids/*pharmacology
MH  - Animals
MH  - Apoptosis/*drug effects
MH  - Cytoprotection/drug effects
MH  - Dexamethasone/*adverse effects
MH  - Mice
MH  - Osteoblasts/*cytology/*drug effects/metabolism
MH  - Quinolizines/*pharmacology
MH  - Tumor Suppressor Protein p53/*metabolism
MH  - Ubiquitin Thiolesterase/*metabolism
MH  - Ubiquitination/drug effects
MH  - Up-Regulation/drug effects
MH  - Matrines
OTO - NOTNLM
OT  - Matrine
OT  - USP14
OT  - dexamethasone
OT  - osteoblast
OT  - ubiquitination
EDAT- 2019/09/17 06:00
MHDA- 2020/02/06 06:00
CRDT- 2019/09/17 06:00
PHST- 2019/09/17 06:00 [entrez]
PHST- 2019/09/17 06:00 [pubmed]
PHST- 2020/02/06 06:00 [medline]
AID - 10.1080/21691401.2019.1664563 [doi]
PST - ppublish
SO  - Artif Cells Nanomed Biotechnol. 2019 Dec;47(1):3720-3728. doi: 
      10.1080/21691401.2019.1664563.