PMID- 31524245
OWN - NLM
STAT- MEDLINE
DCOM- 20200204
LR  - 20200204
IS  - 1791-3004 (Electronic)
IS  - 1791-2997 (Linking)
VI  - 20
IP  - 3
DP  - 2019 Sep
TI  - N‑acetyl cysteine inhibits lipopolysaccharide‑induced apoptosis of human 
      umbilical vein endothelial cells via the p38MAPK signaling pathway.
PG  - 2945-2953
LID - 10.3892/mmr.2019.10526 [doi]
AB  - Lipopolysaccharide (LPS) can regulate the expression of apoptotic factors, 
      including caspase‑3, Bcl‑2 and Bcl‑2‑associated X protein (Bax). Nitric oxide 
      (NO) plays an important role in apoptosis. N‑acetyl cysteine (NAC) has been shown 
      to exhibit antioxidant effects in vitro. However, the effects of NAC on 
      LPS‑induced apoptosis of human umbilical vein endothelial cells (HUVECs) and the 
      associated mechanisms are not well characterized. The present study explored the 
      effect of NAC on LPS‑induced apoptosis of HUVECs and determined the participation 
      of the p38 mitogen‑activated protein kinase (MAPK) pathway in the process of 
      apoptosis. Cell viability was assessed using the Cell Counting Kit‑8 (CCK‑8) 
      assay. The expression of caspase‑3, Bax, Bcl‑2, phosphorylated (p)‑p38MAPK/total 
      (t‑)p38MAPK and p‑endothelial e nitric oxide synthase (eNOS)/t‑eNOS proteins were 
      determined by western blotting. The expression levels of caspase‑3, Bax and Bcl‑2 
      mRNA were determined using reverse transcription‑quantitative polymerase chain 
      reaction (RT‑qPCR). The rate of apoptosis was determined using flow cytometry. An 
      NO detection kit (nitric reductase method) was used to determine NO 
      concentration. The results of CCK‑8 and flow cytometric analyses showed that 
      pretreatment of HUVECs with NAC or p38MAPK inhibitor (SB203580) attenuated 
      LPS‑induced decrease in cell viability and increase in cell apoptosis. RT‑qPCR 
      and western blotting showed that LPS promoted caspase‑3 and Bax expression, but 
      inhibited that of Bcl‑2 in HUVECs; however, these effects were attenuated by 
      pretreatment with NAC or SB203580. LPS stimulation significantly enhanced the 
      expression of p‑p38MAPK protein and reduced the expression of p‑eNOS protein; 
      however, these effects were attenuated by pretreatment with NAC or SB203580. NAC 
      pretreatment attenuated LPS‑induced inhibition of NO synthesis, which was 
      consistent with the effects of SB203580. The results demonstrated that NAC 
      pretreatment alleviated LPS‑induced apoptosis and inhibition of NO production in 
      HUVECs. Furthermore, these effects were proposed to be mediated via the p38MAPK 
      signaling pathway.
FAU - Xiong, Ting
AU  - Xiong T
AD  - Department of Prosthodontics, Hospital of Stomatology Affiliated to Southwest 
      Medical University, Luzhou, Sichuan 646000, P.R. China.
FAU - Zhang, Zhenzhen
AU  - Zhang Z
AD  - Department of Prosthodontics, Hospital of Stomatology Affiliated to Southwest 
      Medical University, Luzhou, Sichuan 646000, P.R. China.
FAU - Zheng, Rui
AU  - Zheng R
AD  - Department of Prosthodontics, Hospital of Stomatology Affiliated to Southwest 
      Medical University, Luzhou, Sichuan 646000, P.R. China.
FAU - Huang, Jialin
AU  - Huang J
AD  - Department of Prosthodontics, Hospital of Stomatology Affiliated to Southwest 
      Medical University, Luzhou, Sichuan 646000, P.R. China.
FAU - Guo, Ling
AU  - Guo L
AD  - Department of Prosthodontics, Hospital of Stomatology Affiliated to Southwest 
      Medical University, Luzhou, Sichuan 646000, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20190725
PL  - Greece
TA  - Mol Med Rep
JT  - Molecular medicine reports
JID - 101475259
RN  - 0 (Biomarkers)
RN  - 0 (Lipopolysaccharides)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - WYQ7N0BPYC (Acetylcysteine)
SB  - IM
MH  - Acetylcysteine/*pharmacology
MH  - Apoptosis/*drug effects
MH  - Biomarkers
MH  - Cell Survival/drug effects
MH  - Human Umbilical Vein Endothelial Cells/*drug effects/*metabolism
MH  - Humans
MH  - Lipopolysaccharides/*pharmacology
MH  - MAP Kinase Signaling System/*drug effects
MH  - Nitric Oxide/metabolism
MH  - Phosphorylation/drug effects
EDAT- 2019/09/17 06:00
MHDA- 2020/02/06 06:00
CRDT- 2019/09/17 06:00
PHST- 2019/02/25 00:00 [received]
PHST- 2019/07/05 00:00 [accepted]
PHST- 2019/09/17 06:00 [pubmed]
PHST- 2020/02/06 06:00 [medline]
PHST- 2019/09/17 06:00 [entrez]
AID - 10.3892/mmr.2019.10526 [doi]
PST - ppublish
SO  - Mol Med Rep. 2019 Sep;20(3):2945-2953. doi: 10.3892/mmr.2019.10526. Epub 2019 Jul 
      25.