PMID- 31524257
OWN - NLM
STAT- MEDLINE
DCOM- 20200204
LR  - 20200225
IS  - 1791-3004 (Electronic)
IS  - 1791-2997 (Print)
IS  - 1791-2997 (Linking)
VI  - 20
IP  - 3
DP  - 2019 Sep
TI  - Identification of core genes and pathways in type 2 diabetes mellitus by 
      bioinformatics analysis.
PG  - 2597-2608
LID - 10.3892/mmr.2019.10522 [doi]
AB  - Type 2 diabetes mellitus (T2DM) is a metabolic disorder. Numerous proteins have 
      been identified that are associated with the occurrence and development of T2DM. 
      This study aimed to identify potential core genes and pathways involved in T2DM, 
      through exhaustive bioinformatic analyses using GSE20966 microarray profiles of 
      pancreatic beta‑cells obtained from healthy controls and patients with T2DM. The 
      original microarray data were downloaded from the Gene Expression Omnibus 
      database. Data were processed by the limma package in R software and the 
      differentially expressed genes (DEGs) were identified. Gene Ontology functional 
      analysis and Kyoto Encyclopedia of Genes and Genomes pathway analysis were 
      carried out to identify potential biological functions and pathways of the DEGs. 
      Key transcription factors were identified using the WEB‑based GEne SeT AnaLysis 
      Toolkit (WebGestalt) and Enrichr. The Search Tool for the Retrieval of 
      Interacting Genes (STRING) database was used to establish a protein‑protein 
      interaction (PPI) network for the DEGs. In total, 329 DEGs were involved in T2DM, 
      with 208 upregulated genes enriched in pancreatic secretion and the complement 
      and coagulation cascades, and 121 downregulated genes enriched in insulin 
      secretion, carbohydrate digestion and absorption, and the Toll‑like receptor 
      pathway. Furthermore, hepatocyte nuclear factor 1‑alpha (HNF1A), signal 
      transducer and activator of transcription 3 (STAT3) and glucocorticoid receptor 
      (GR) were key transcription factors in T2DM. Twenty important nodes were detected 
      in the PPI network. Finally, two core genes, serpin family G member 1 (SERPING1) 
      and alanyl aminopeptidase, membrane (ANPEP), were shown to be associated with the 
      development of T2DM. On the whole, the findings of this study enhance our 
      understanding of the potential molecular mechanisms of T2DM and provide potential 
      targets for further research.
FAU - Ding, Linchao
AU  - Ding L
AD  - Department of Scientific Research, Jinhua Hospital, Zhejiang University School of 
      Medicine, Jinhua, Zhejiang 321000, P.R. China.
FAU - Fan, Lei
AU  - Fan L
AD  - Department of Pharmacy, Jinhua Hospital, Zhejiang University School of Medicine, 
      Jinhua, Zhejiang 321000, P.R. China.
FAU - Xu, Xiaodong
AU  - Xu X
AD  - Department of Endocrinology, Jinhua Hospital, Zhejiang University School of 
      Medicine, Jinhua, Zhejiang 321000, P.R. China.
FAU - Fu, Jianfei
AU  - Fu J
AD  - Department of Scientific Research, Jinhua Hospital, Zhejiang University School of 
      Medicine, Jinhua, Zhejiang 321000, P.R. China.
FAU - Xue, Yadong
AU  - Xue Y
AD  - Department of Scientific Research, Jinhua Hospital, Zhejiang University School of 
      Medicine, Jinhua, Zhejiang 321000, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20190724
PL  - Greece
TA  - Mol Med Rep
JT  - Molecular medicine reports
JID - 101475259
RN  - 0 (Biomarkers)
SB  - IM
MH  - Animals
MH  - Biomarkers
MH  - Cell Line
MH  - *Computational Biology/methods
MH  - Databases, Genetic
MH  - Diabetes Mellitus, Type 2/*genetics/*metabolism
MH  - Disease Susceptibility
MH  - *Gene Expression Regulation
MH  - Gene Ontology
MH  - Gene Regulatory Networks
MH  - Humans
MH  - Mice
MH  - *Signal Transduction
PMC - PMC6691242
EDAT- 2019/09/17 06:00
MHDA- 2020/02/06 06:00
PMCR- 2019/07/24
CRDT- 2019/09/17 06:00
PHST- 2019/01/10 00:00 [received]
PHST- 2019/05/07 00:00 [accepted]
PHST- 2019/09/17 06:00 [pubmed]
PHST- 2020/02/06 06:00 [medline]
PHST- 2019/09/17 06:00 [entrez]
PHST- 2019/07/24 00:00 [pmc-release]
AID - mmr-20-03-2597 [pii]
AID - 10.3892/mmr.2019.10522 [doi]
PST - ppublish
SO  - Mol Med Rep. 2019 Sep;20(3):2597-2608. doi: 10.3892/mmr.2019.10522. Epub 2019 Jul 
      24.