PMID- 31529740
OWN - NLM
STAT- MEDLINE
DCOM- 20200225
LR  - 20200225
IS  - 1365-2672 (Electronic)
IS  - 1364-5072 (Linking)
VI  - 128
IP  - 1
DP  - 2020 Jan
TI  - Immune gene expression in gilthead seabream (Sparus aurata) after Lymphocystis 
      disease virus (LCDV-Sa) challenge resulting in asymptomatic infection.
PG  - 41-53
LID - 10.1111/jam.14454 [doi]
AB  - AIMS: To determine the immune gene expression response of gilthead seabream 
      (Sparus aurata) that is experimentally infected with the lymphocystivirus 
      LCDV-Sa. METHODS AND RESULTS: Viral DNA and transcripts were detected by qPCR in 
      all samples from fish injected with LCDV-Sa, demonstrating that the virus 
      establish a systemic and asymptomatic infection. The expression of 23 
      immune-related genes was also analysed by RT-qPCR in the head kidney (HK) and 
      intestine at several times post-infection (dpi). In HK, the expression of five 
      type I interferon (IFN)-related genes (ifn, irf3, mx2, mx3 and isg15), il10 and 
      ck10 was upregulated at 1-3 dpi, while genes related to the inflammation process 
      (tnfalpha, il1ss, il6, casp1) were not differentially expressed or even downregulated. 
      The expression profile in the intestine was different regarding type I 
      INF-related genes. An upregulated c3 and ighm expression was observed in both HK 
      and intestine at 3-8 dpi. Finally, the transcription of nccrp1 and mhcIIalpha was 
      induced in HK, whereas tcrbeta expression was downregulated in both organs. 
      CONCLUSIONS: LCDV-Sa seems to trigger an immune response in gilthead seabream 
      characterized by a partial activation of type I IFN system and a lack of systemic 
      inflammatory response which may be related to viral persistence. SIGNIFICANCE AND 
      IMPACT OF THE STUDY: The immune response observed in gilthead seabream infected 
      by LCDV-Sa could be implicated in the establishment of an asymptomatic persistent 
      infection.
CI  - (c) 2019 The Society for Applied Microbiology.
FAU - Leiva-Rebollo, R
AU  - Leiva-Rebollo R
AD  - Departamento de Microbiologia, Universidad de Malaga, Malaga, Spain.
FAU - Labella, A M
AU  - Labella AM
AD  - Departamento de Microbiologia, Universidad de Malaga, Malaga, Spain.
FAU - Borrego, J J
AU  - Borrego JJ
AUID- ORCID: 0000-0002-2174-0652
AD  - Departamento de Microbiologia, Universidad de Malaga, Malaga, Spain.
FAU - Castro, D
AU  - Castro D
AUID- ORCID: 0000-0002-1589-3820
AD  - Departamento de Microbiologia, Universidad de Malaga, Malaga, Spain.
LA  - eng
SI  - GENBANK/KF857324
SI  - GENBANK/AY751798
SI  - GENBANK/AY962254
SI  - GENBANK/AM920661
SI  - GENBANK/FJ490556
SI  - GENBANK/FJ490555
SI  - GENBANK/FJ652200
SI  - GENBANK/AJ413189
SI  - GENBANK/AJ277166
SI  - GENBANK/EU244588
SI  - GENBANK/JX976621
SI  - GENBANK/AM490060
SI  - GENBANK/GU181394
SI  - GENBANK/GU181390
SI  - GENBANK/HM543456
SI  - GENBANK/AY651258
SI  - GENBANK/AM261209
SI  - GENBANK/AM493677
SI  - GENBANK/DQ019401
SI  - GENBANK/AF384096
SI  - GENBANK/AF184170
GR  - P12-RNM-2261/Junta de Andalucia/
PT  - Journal Article
DEP - 20191003
PL  - England
TA  - J Appl Microbiol
JT  - Journal of applied microbiology
JID - 9706280
RN  - 0 (Cytokines)
RN  - 0 (Fish Proteins)
SB  - IM
MH  - Animals
MH  - *Asymptomatic Infections
MH  - Cytokines/genetics
MH  - DNA Virus Infections/immunology/*veterinary/virology
MH  - Fish Diseases/*immunology/virology
MH  - Fish Proteins/genetics
MH  - Gene Expression Regulation/*immunology
MH  - Head Kidney/immunology
MH  - Intestines/immunology
MH  - Iridoviridae/*physiology
MH  - Sea Bream/genetics/*immunology/virology
OTO - NOTNLM
OT  - asymptomatic infection
OT  - gilthead seabream (Sparus aurata)
OT  - immune gene expression
OT  - lymphocystis disease virus
OT  - viral challenge
EDAT- 2019/09/19 06:00
MHDA- 2020/02/26 06:00
CRDT- 2019/09/19 06:00
PHST- 2019/05/30 00:00 [received]
PHST- 2019/08/04 00:00 [revised]
PHST- 2019/09/04 00:00 [accepted]
PHST- 2019/09/19 06:00 [pubmed]
PHST- 2020/02/26 06:00 [medline]
PHST- 2019/09/19 06:00 [entrez]
AID - 10.1111/jam.14454 [doi]
PST - ppublish
SO  - J Appl Microbiol. 2020 Jan;128(1):41-53. doi: 10.1111/jam.14454. Epub 2019 Oct 3.