PMID- 31529971
OWN - NLM
STAT- MEDLINE
DCOM- 20200409
LR  - 20200409
IS  - 1559-4106 (Electronic)
IS  - 1559-4106 (Linking)
VI  - 14
IP  - 5
DP  - 2019 Sep 17
TI  - Improved ion imaging of slowly dried neurons and skin cells by graphene cover in 
      time-of-flight secondary ion mass spectrometry.
PG  - 051001
LID - 10.1116/1.5118259 [doi]
AB  - Time-of-flight secondary ion mass spectrometry (ToF-SIMS) is a powerful tool to 
      obtain both chemical information and spatial distribution of specific molecules 
      of interest on a specimen surface. However, since the focused ion beam requires 
      ultrahigh vacuum conditions for desorption and ionization of analytes, proper 
      specimen preparation, such as drying, freeze-drying, and frozen dehydration, is 
      required for ToF-SIMS analysis. In particular, biological specimens with high 
      moisture content generally have a problem of specimen deformation during the 
      normal drying process for a vacuum environment. In this study, the authors 
      propose a cellular specimen preparation method to improve the ion imaging of 
      cells by reducing the deformation of specimens in ToF-SIMS analysis. When the 
      cells on the slide substrate are completely covered with single-layer graphene, 
      the ToF-SIMS imaging is improved by reduced cell deformation due to slow drying. 
      In addition, the graphene encapsulation also induces a reduction in the yield of 
      secondary ions, thereby suppressing the background ion spectra generated by the 
      unwanted organic residues on the substrate, resulting in the improvement of 
      ToF-SIMS imaging. The authors also found that adding plasma treatment to this 
      sample preparation can further improve ion imaging of cells. After cell 
      dehydration is completed, the covered graphene layer can be peeled off by 
      air-plasma treatment and the unwanted organic residues on the substrate can be 
      removed due to plasma cleaning, thereby much improving ion imaging of cells.
FAU - Lee, Sun Young
AU  - Lee SY
AD  - Division of Technology Business, National Institute for Nanomaterials Technology 
      (NINT), Pohang University of Science and Technology (POSTECH), 77 Cheongam-Ro, 
      Nam-Gu, Pohang, Gyeongbuk 37673, Republic of Korea.
FAU - Lim, Heejin
AU  - Lim H
AD  - Department of New Biology, Daegu Gyeongbuk Institute of Science and Technology 
      (DGIST), 333 Techno Jungang Daero, Hyeonpung-Eup, Dalseong-Gun, Daegu 42988, 
      Republic of Korea.
FAU - Moon, Dae Won
AU  - Moon DW
AD  - Department of New Biology, Daegu Gyeongbuk Institute of Science and Technology 
      (DGIST), 333 Techno Jungang Daero, Hyeonpung-Eup, Dalseong-Gun, Daegu 42988, 
      Republic of Korea.
FAU - Kim, Jae Young
AU  - Kim JY
AUID- ORCID: 0000000316795736
AD  - Department of New Biology and Department of Robotics Engineering, Daegu Gyeongbuk 
      Institute of Science and Technology (DGIST), 333 Techno Jungang Daero, 
      Hyeonpung-Eup, Dalseong-Gun, Daegu 42988, Republic of Korea.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190917
PL  - United States
TA  - Biointerphases
JT  - Biointerphases
JID - 101275679
RN  - 7782-42-5 (Graphite)
SB  - IM
MH  - Animals
MH  - Desiccation
MH  - Graphite/*chemistry
MH  - *Neurons/chemistry/cytology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - *Skin/chemistry/diagnostic imaging
MH  - Spectrometry, Mass, Secondary Ion
EDAT- 2019/09/19 06:00
MHDA- 2020/04/10 06:00
CRDT- 2019/09/19 06:00
PHST- 2019/09/19 06:00 [entrez]
PHST- 2019/09/19 06:00 [pubmed]
PHST- 2020/04/10 06:00 [medline]
AID - 10.1116/1.5118259 [doi]
PST - epublish
SO  - Biointerphases. 2019 Sep 17;14(5):051001. doi: 10.1116/1.5118259.