PMID- 31531565
OWN - NLM
STAT- MEDLINE
DCOM- 20190930
LR  - 20191210
IS  - 1807-3107 (Electronic)
IS  - 1806-8324 (Linking)
VI  - 33
DP  - 2019 Sep 9
TI  - Effect of stem cells combined with a polymer/ceramic membrane on osteoporotic 
      bone repair.
PG  - e079
LID - S1806-83242019000100252 [pii]
LID - 10.1590/1807-3107BOR-2019.vol33.0079 [doi]
AB  - Cell therapy associated with guided bone regeneration (GBR) can be used to treat 
      bone defects under challenging conditions such as osteoporosis. This study aimed 
      to evaluate the effect of mesenchymal stem cells (MSCs) in combination with a 
      poly(vinylidene-trifluoroethylene)/barium titanate (PVDF-TrFE/BT) membrane on 
      bone repair in osteoporotic rats. Osteoporosis was induced in female rats by 
      bilateral removal of the ovaries (OVX) or sham surgery (SHAM), and the 
      osteoporotic condition was characterized after 5 months by microtomographic and 
      morphometric analyses. Calvarial defects were created in osteoporotic rats that 
      immediately received the PVDF-TrFE/BT membrane. After 2 weeks, bone 
      marrow-derived MSCs from healthy rats, characterized by the expression of surface 
      markers using flow cytometry, or phosphate-buffered saline (PBS) (Control) were 
      injected into the defects and bone formation was evaluated 4 weeks post-injection 
      by microtomographic, morphometric, and histological analyses. A reduction in the 
      amount of bone tissue in the femurs of OVX compared with SHAM rats confirmed the 
      osteoporotic condition of the experimental model. More bone formation was 
      observed when the defects were injected with MSCs compared to that with PBS. The 
      modification that we are proposing in this study for the classical GBR approach 
      where cells are locally injected after a membrane implantation may be a promising 
      therapeutic strategy to increase bone formation under osteoporotic condition.
FAU - Almeida, Adriana Luisa Goncalves
AU  - Almeida ALG
AUID- ORCID: 0000-0001-9360-3027
AD  - Universidade de Sao Paulo - USP, School of Dentistry of Ribeirao Preto , Bone 
      Research Lab , Ribeirao Preto , SP , Brazil .
FAU - Freitas, Gileade Pereira
AU  - Freitas GP
AUID- ORCID: 0000-0002-1674-4667
AD  - Universidade de Sao Paulo - USP, School of Dentistry of Ribeirao Preto , Bone 
      Research Lab , Ribeirao Preto , SP , Brazil .
FAU - Lopes, Helena Bacha
AU  - Lopes HB
AUID- ORCID: 0000-0002-6998-5296
AD  - Universidade de Sao Paulo - USP, School of Dentistry of Ribeirao Preto , Bone 
      Research Lab , Ribeirao Preto , SP , Brazil .
FAU - Gimenes, Rossano
AU  - Gimenes R
AUID- ORCID: 0000-0002-4318-6330
AD  - Universidade Federal de Itajuba - Unifei, Institute of Physics and Chemistry , 
      Itajuba , MG , Brazil.
FAU - Siessere, Selma
AU  - Siessere S
AUID- ORCID: 0000-0001-9756-3771
AD  - Universidade de Sao Paulo - USP, School of Dentistry of Ribeirao Preto , 
      Department of Basic and Oral Biology , Ribeirao Preto , SP , Brazil .
FAU - Sousa, Luiz Gustavo
AU  - Sousa LG
AUID- ORCID: 0000-0002-2090-4029
AD  - Universidade de Sao Paulo - USP, School of Dentistry of Ribeirao Preto , 
      Department of Basic and Oral Biology , Ribeirao Preto , SP , Brazil .
FAU - Beloti, Marcio Mateus
AU  - Beloti MM
AUID- ORCID: 0000-0003-0149-7189
AD  - Universidade de Sao Paulo - USP, School of Dentistry of Ribeirao Preto , Bone 
      Research Lab , Ribeirao Preto , SP , Brazil .
FAU - Rosa, Adalberto Luiz
AU  - Rosa AL
AUID- ORCID: 0000-0002-6495-2778
AD  - Universidade de Sao Paulo - USP, School of Dentistry of Ribeirao Preto , Bone 
      Research Lab , Ribeirao Preto , SP , Brazil .
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
DEP - 20190909
PL  - Brazil
TA  - Braz Oral Res
JT  - Brazilian oral research
JID - 101307187
RN  - 0 (Barium Compounds)
RN  - 0 (Polyvinyls)
RN  - 28960-88-5 (vinylidene fluoride-trifluoroethylene copolymer)
RN  - D1JT611TNE (Titanium)
SB  - IM
MH  - Animals
MH  - Barium Compounds/chemistry/*pharmacology
MH  - Bone Density
MH  - Bone Regeneration/drug effects/physiology
MH  - Female
MH  - Flow Cytometry
MH  - Guided Tissue Regeneration/*methods
MH  - Imaging, Three-Dimensional
MH  - Mesenchymal Stem Cells/chemistry/*physiology
MH  - Osteogenesis/*drug effects/physiology
MH  - Osteoporosis/physiopathology/*therapy
MH  - Ovariectomy
MH  - Polyvinyls/chemistry/*pharmacology
MH  - Random Allocation
MH  - Rats, Wistar
MH  - Reproducibility of Results
MH  - Time Factors
MH  - Titanium/chemistry/*pharmacology
MH  - Treatment Outcome
EDAT- 2019/09/19 06:00
MHDA- 2019/10/01 06:00
CRDT- 2019/09/19 06:00
PHST- 2019/02/22 00:00 [received]
PHST- 2019/06/06 00:00 [accepted]
PHST- 2019/09/19 06:00 [entrez]
PHST- 2019/09/19 06:00 [pubmed]
PHST- 2019/10/01 06:00 [medline]
AID - S1806-83242019000100252 [pii]
AID - 10.1590/1807-3107BOR-2019.vol33.0079 [doi]
PST - epublish
SO  - Braz Oral Res. 2019 Sep 9;33:e079. doi: 10.1590/1807-3107BOR-2019.vol33.0079.