PMID- 31532045
OWN - NLM
STAT- MEDLINE
DCOM- 20200514
LR  - 20200514
IS  - 1439-0507 (Electronic)
IS  - 0933-7407 (Linking)
VI  - 63
IP  - 1
DP  - 2020 Jan
TI  - Evaluation of (1 --> 3)-beta-D-glucan assay for diagnosing paracoccidioidomycosis.
PG  - 38-42
LID - 10.1111/myc.13007 [doi]
AB  - BACKGROUND: Paracoccidioidomycosis (PCM) is highly prevalent in Latin America, 
      but no commercial system is available for diagnosing this endemic mycosis. 
      OBJECTIVES: To check the performance of (1 --> 3)-beta-D-glucan assay (BDG) for 
      diagnosing  PCM in 29 patients with proven fungal disease and compared with 
      double immunodiffusion assay for detecting anti-Paracoccidioides antibodies. 
      PATIENTS AND METHODS: We selected 52 serum samples sequentially obtained from 29 
      patients with active PCM (12 chronic and 17 acute form). Samples were collected 
      at baseline, and for 16 patients, additional serum levels were obtained after 3 
      and 6 months of antifungal treatment. Detection of BDG in serum was performed by 
      using the Fungitell((R)) assay. For the double immunodiffusion assay, 
      Paracoccidioides exoantigen was used in latex agglutination tests to detect serum 
      anti-Paracoccidioides antibodies. RESULTS: Despite exhibiting good sensitivity in 
      the diagnosis of patients with PCM, we failed to demonstrate any correlation 
      between the postdiagnosis kinetic profile of BDG serum levels and clinical 
      response to antifungal therapy. This finding may be related to the maintenance of 
      quiescent foci of fungal infection in several organs and tissues, a phenomenon 
      that has been previously reported by other authors and helps to understand why so 
      many relapses are documented in patients treated for short periods of time. 
      Finally, we did not find any correlation between BDG quantification and specific 
      anti-P brasiliensis antibodies serum titres in patients with PCM. CONCLUSIONS: In 
      conclusion, BDG is detected in serum samples of most patients with PCM but is 
      probably not useful for predicting clinical response to antifungal therapy.
CI  - (c) 2019 Blackwell Verlag GmbH.
FAU - Melo, Analy Salles de Azevedo
AU  - Melo ASA
AUID- ORCID: 0000-0001-6900-6468
AD  - Laboratorio Especial de Micologia, Departamento de Medicina, Universidade Federal 
      de Sao Paulo (UNIFESP), Sao Paulo, Brasil.
FAU - Santos, Daniel Wagner de Castro Lima
AU  - Santos DWCL
AUID- ORCID: 0000-0003-4946-911X
AD  - Laboratorio Especial de Micologia, Departamento de Medicina, Universidade Federal 
      de Sao Paulo (UNIFESP), Sao Paulo, Brasil.
FAU - Lima, Soraia Lopes
AU  - Lima SL
AD  - Laboratorio Especial de Micologia, Departamento de Medicina, Universidade Federal 
      de Sao Paulo (UNIFESP), Sao Paulo, Brasil.
FAU - Rodrigues, Anderson Messias
AU  - Rodrigues AM
AUID- ORCID: 0000-0003-0891-4563
AD  - Laboratorio de Patogenos Fungicos Emergentes, Departamento de Microbiologia, 
      Imunologia e Parasitologia, Universidade Federal de Sao Paulo (UNIFESP), Sao 
      Paulo, Brazil.
FAU - de Camargo, Zoilo Pires
AU  - de Camargo ZP
AD  - Laboratorio de Micologia Medica, Departamento de Microbiologia, Imunologia e 
      Parasitologia, Universidade Federal de Sao Paulo (UNIFESP), Sao Paulo, Brazil.
FAU - Finkelman, Malcolm
AU  - Finkelman M
AD  - Associates of Cape Cod, Clinical Development Department, Research Laboratory, 
      Falmouth, MA, USA.
FAU - Colombo, Arnaldo Lopes
AU  - Colombo AL
AUID- ORCID: 0000-0003-0793-8491
AD  - Laboratorio Especial de Micologia, Departamento de Medicina, Universidade Federal 
      de Sao Paulo (UNIFESP), Sao Paulo, Brasil.
LA  - eng
GR  - 307510/2015-8/Conselho Nacional de Desenvolvimento Cientifico e Tecnologico/
PT  - Journal Article
DEP - 20191105
PL  - Germany
TA  - Mycoses
JT  - Mycoses
JID - 8805008
RN  - 0 (Antibodies, Fungal)
RN  - 0 (Antifungal Agents)
RN  - 0 (Antigens, Fungal)
RN  - 0 (Glucans)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Antibodies, Fungal/blood
MH  - Antifungal Agents/therapeutic use
MH  - Antigens, Fungal/immunology
MH  - Child
MH  - Child, Preschool
MH  - Enzyme-Linked Immunosorbent Assay/methods
MH  - Female
MH  - Glucans/immunology
MH  - Humans
MH  - Infant
MH  - Infant, Newborn
MH  - Latin America
MH  - Male
MH  - Middle Aged
MH  - Paracoccidioides/*immunology
MH  - Paracoccidioidomycosis/*diagnosis/drug therapy/microbiology
MH  - Young Adult
OTO - NOTNLM
OT  - (1 --> 3)-beta-D-glucan assay
OT  - Paracoccidioidomycosis
OT  - antifungal therapy
OT  - endemic mycosis
OT  - fungal biomarker
EDAT- 2019/09/19 06:00
MHDA- 2020/05/15 06:00
CRDT- 2019/09/19 06:00
PHST- 2019/03/21 00:00 [received]
PHST- 2019/09/12 00:00 [revised]
PHST- 2019/09/13 00:00 [accepted]
PHST- 2019/09/19 06:00 [pubmed]
PHST- 2020/05/15 06:00 [medline]
PHST- 2019/09/19 06:00 [entrez]
AID - 10.1111/myc.13007 [doi]
PST - ppublish
SO  - Mycoses. 2020 Jan;63(1):38-42. doi: 10.1111/myc.13007. Epub 2019 Nov 5.