PMID- 31532642
OWN - NLM
STAT- MEDLINE
DCOM- 20200610
LR  - 20200610
IS  - 1520-4995 (Electronic)
IS  - 0006-2960 (Linking)
VI  - 58
IP  - 40
DP  - 2019 Oct 8
TI  - Definition of the Interaction Domain and Electron Transfer Route between 
      Cytochrome c and Cytochrome Oxidase.
PG  - 4125-4135
LID - 10.1021/acs.biochem.9b00646 [doi]
AB  - The reaction between cytochrome c (Cc) and cytochrome c oxidase (CcO) was studied 
      using horse cytochrome c derivatives labeled with ruthenium trisbipyridine at Cys 
      39 (Ru-39-Cc). Flash photolysis of a 1:1 complex between Ru-39-Cc and bovine CcO 
      at a low ionic strength resulted in the electron transfer from photoreduced heme 
      c to Cu(A) with an intracomplex rate constant of k(3) = 6 x 10(4) s(-1). The 
      K13A, K72A, K86A, and K87A Ru-39-Cc mutants had nearly the same k(3) value but 
      bound much more weakly to bovine CcO than wild-type Ru-39-Cc, indicating that 
      lysines 13, 72, 86, and 87 were involved in electrostatic binding to CcO, but 
      were not involved in the electron transfer pathway. The Rhodobacter sphaeroides 
      (Rs) W143F mutant (bovine W104) caused a 450-fold decrease in k(3) but did not 
      affect the binding strength with CcO or the redox potential of Cu(A). These 
      results are consistent with a computational model for Cc-CcO (Roberts and Pique ( 
      1999 ) J. Biol. Chem. 274 , 38051 - 38060 ) with the following electron transfer 
      pathway: heme c --> CcO-W104 --> CcO-M207 --> Cu(A). A crystal structure for the Cc-CcO 
      complex with the proposed electron transfer pathway heme c --> Cc-C14 --> Cc-K13 --> 
      CcO-Y105 --> CcO-M207 --> Cu(A) ( S. Shimada ( 2017 ) EMBO J. 36 , 291 - 300 ) is not 
      consistent with the kinetic results because the K13A mutation had no effect on 
      k(3). Addition of 40% ethylene glycol (as present during the crystal preparation) 
      decreased k(3) significantly, indicating that it affected the conformation of the 
      complex. This may explain the discrepancy between the current results and the 
      crystallographic structure.
FAU - Scharlau, Martha
AU  - Scharlau M
AD  - Department of Chemistry and Biochemistry , University of Arkansas , Fayetteville 
      , Arkansas 72701 , United States.
FAU - Geren, Lois
AU  - Geren L
AD  - Department of Chemistry and Biochemistry , University of Arkansas , Fayetteville 
      , Arkansas 72701 , United States.
FAU - Zhen, Eugene Y
AU  - Zhen EY
AD  - Department of Biochemistry and Molecular Biology , Michigan State University , 
      East Lansing , Michigan 48824 , United States.
FAU - Ma, Ling
AU  - Ma L
AD  - Department of Chemistry and Biochemistry , University of Arkansas , Fayetteville 
      , Arkansas 72701 , United States.
FAU - Rajagukguk, Ray
AU  - Rajagukguk R
AD  - Department of Chemistry and Biochemistry , University of Arkansas , Fayetteville 
      , Arkansas 72701 , United States.
FAU - Ferguson-Miller, Shelagh
AU  - Ferguson-Miller S
AD  - Department of Biochemistry and Molecular Biology , Michigan State University , 
      East Lansing , Michigan 48824 , United States.
FAU - Durham, Bill
AU  - Durham B
AD  - Department of Chemistry and Biochemistry , University of Arkansas , Fayetteville 
      , Arkansas 72701 , United States.
FAU - Millett, Francis
AU  - Millett F
AUID- ORCID: 0000-0002-1352-5768
AD  - Department of Chemistry and Biochemistry , University of Arkansas , Fayetteville 
      , Arkansas 72701 , United States.
LA  - eng
GR  - P30 GM103450/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20190926
PL  - United States
TA  - Biochemistry
JT  - Biochemistry
JID - 0370623
RN  - 0 (Coordination Complexes)
RN  - 42VZT0U6YR (Heme)
RN  - 789U1901C5 (Copper)
RN  - 7UI0TKC3U5 (Ruthenium)
RN  - 9007-43-6 (Cytochromes c)
RN  - EC 1.9.3.1 (Electron Transport Complex IV)
RN  - FC72KVT52F (Ethylene Glycol)
SB  - IM
MH  - Animals
MH  - Cattle
MH  - Coordination Complexes/chemistry
MH  - Copper/chemistry
MH  - Cytochromes c/*chemistry/genetics
MH  - Electron Transport
MH  - Electron Transport Complex IV/*chemistry
MH  - Ethylene Glycol/chemistry
MH  - Heme/chemistry
MH  - Horses
MH  - Mutation
MH  - Photolysis
MH  - Protein Domains
MH  - Ruthenium/chemistry/radiation effects
EDAT- 2019/09/19 06:00
MHDA- 2020/06/11 06:00
CRDT- 2019/09/19 06:00
PHST- 2019/09/19 06:00 [pubmed]
PHST- 2020/06/11 06:00 [medline]
PHST- 2019/09/19 06:00 [entrez]
AID - 10.1021/acs.biochem.9b00646 [doi]
PST - ppublish
SO  - Biochemistry. 2019 Oct 8;58(40):4125-4135. doi: 10.1021/acs.biochem.9b00646. Epub 
      2019 Sep 26.