PMID- 31532919
OWN - NLM
STAT- MEDLINE
DCOM- 20200713
LR  - 20200713
IS  - 1934-8576 (Electronic)
IS  - 1934-8576 (Linking)
VI  - 89
IP  - 1
DP  - 2019 Sep
TI  - A Rapid Neurological Assessment Protocol for Repeated Mild Traumatic Brain Injury 
      in Awake Rats.
PG  - e80
LID - 10.1002/cpns.80 [doi]
AB  - Preclinical models for mild traumatic brain injury (mTBI) need to recapitulate 
      several essential clinical features associated with mTBI, including a lack of 
      significant neuropathology and the onset of neurocognitive symptoms normally 
      associated with mTBI. Here we show how to establish a protocol for reliably and 
      repeatedly inducing a mild awake closed head injury (ACHI) in rats, with no 
      mortality or clinical indications of persistent pain. Moreover, we implement a 
      new rapid neurological assessment protocol (NAP) that can be completely conducted 
      within 1 min of each impact. This ACHI model will help to rectify the paucity of 
      data on how repeated mTBI (r-mTBI) impacts the juvenile brain, an area of 
      significant concern in clinical populations where there is evidence that 
      behavioral sequelae following injury can be more persistent in juveniles. In 
      addition, the ACHI model can help determine if r-mTBI early in life can 
      predispose the brain to exhibiting greater neuropathology (i.e., chronic 
      traumatic encephalopathy) later in life and can facilitate the identification of 
      critical periods of vulnerability to r-mTBI across the lifespan. This article 
      describes the protocol for administering an awake closed head mTBI (i.e., ACHI) 
      to rats, as well as how to perform a rapid NAP following each ACHI. Methods for 
      administering the ACHI to individual subjects repeatedly are described, as are 
      the methods and scoring system for the NAP. The goal of this article is to 
      provide a standardized set of procedures allowing the ACHI and NAP protocols to 
      be used reliably by different laboratories. (c) 2019 by John Wiley & Sons, Inc.
CI  - (c) 2019 John Wiley & Sons, Inc.
FAU - Christie, Brian R
AU  - Christie BR
AD  - Division of Medical Sciences, University of Victoria, Victoria, British Columbia, 
      Canada.
AD  - Island Medical Program, University of British Columbia, Vancouver, British 
      Columbia, Canada.
FAU - Trivino-Paredes, Juan
AU  - Trivino-Paredes J
AD  - Division of Medical Sciences, University of Victoria, Victoria, British Columbia, 
      Canada.
AD  - Island Medical Program, University of British Columbia, Vancouver, British 
      Columbia, Canada.
FAU - Pinar, Cristina
AU  - Pinar C
AD  - Division of Medical Sciences, University of Victoria, Victoria, British Columbia, 
      Canada.
AD  - Island Medical Program, University of British Columbia, Vancouver, British 
      Columbia, Canada.
FAU - Neale, Katie J
AU  - Neale KJ
AD  - Division of Medical Sciences, University of Victoria, Victoria, British Columbia, 
      Canada.
AD  - Island Medical Program, University of British Columbia, Vancouver, British 
      Columbia, Canada.
FAU - Meconi, Alicia
AU  - Meconi A
AD  - Division of Medical Sciences, University of Victoria, Victoria, British Columbia, 
      Canada.
AD  - Island Medical Program, University of British Columbia, Vancouver, British 
      Columbia, Canada.
FAU - Reid, Hannah
AU  - Reid H
AD  - Division of Medical Sciences, University of Victoria, Victoria, British Columbia, 
      Canada.
AD  - Island Medical Program, University of British Columbia, Vancouver, British 
      Columbia, Canada.
FAU - Hutton, Craig P
AU  - Hutton CP
AD  - Division of Medical Sciences, University of Victoria, Victoria, British Columbia, 
      Canada.
AD  - Island Medical Program, University of British Columbia, Vancouver, British 
      Columbia, Canada.
LA  - eng
GR  - CIHR/Canada
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Curr Protoc Neurosci
JT  - Current protocols in neuroscience
JID - 9706581
SB  - IM
MH  - Animals
MH  - Brain/*surgery
MH  - Brain Concussion/*surgery
MH  - Disease Models, Animal
MH  - *Neurologic Examination/instrumentation/methods
MH  - Rats
MH  - Time Factors
MH  - Wakefulness/*physiology
OTO - NOTNLM
OT  - behavior
OT  - concussion
OT  - head trauma
OT  - mild traumatic brain injury
EDAT- 2019/09/19 06:00
MHDA- 2020/07/14 06:00
CRDT- 2019/09/19 06:00
PHST- 2019/09/19 06:00 [entrez]
PHST- 2019/09/19 06:00 [pubmed]
PHST- 2020/07/14 06:00 [medline]
AID - 10.1002/cpns.80 [doi]
PST - ppublish
SO  - Curr Protoc Neurosci. 2019 Sep;89(1):e80. doi: 10.1002/cpns.80.