PMID- 31533222 OWN - NLM STAT- MEDLINE DCOM- 20200211 LR - 20200211 IS - 1420-3049 (Electronic) IS - 1420-3049 (Linking) VI - 24 IP - 18 DP - 2019 Sep 17 TI - Screening 89 Pesticides in Fishery Drugs by Ultrahigh Performance Liquid Chromatography Tandem Quadrupole-Orbitrap Mass Spectrometer. LID - 10.3390/molecules24183375 [doi] LID - 3375 AB - Multiclass screening of drugs with high resolution mass spectrometry is of great interest due to its high time-efficiency and excellent accuracy. A high-scale, fast screening method for pesticides in fishery drugs was established based on ultrahigh performance liquid chromatography tandem quadrupole-Orbitrap high-resolution mass spectrometer. The target compounds - were diluted in methanol and extracted by ultrasonic treatment, and the extracts were diluted with MeOH-water (1:1, v/v) and centrifuged to remove impurities. The chromatographic separation was performed on an Accucore aQ-MS column (100 mm x 2.1 mm, 2.6 mum) with gradient elution using 0.1% formic acid in water (containing 5 mmol/L ammonium formate) and 0.1% formic acid in methanol (containing 5 mmol/L ammonium formate) in Full Scan/dd-MS2 (TopN) scan mode. A screening database, including mass spectrometric and chromatographic information, was established for identification of compounds. The screening detection limits of methods ranged between 1-500 mg/kg, the recoveries of real samples spiked with the concentration of 10 mg/kg and 100 mg/kg standard mixture ranged from 70% to 110% for more than sixty compounds, and the relative standard deviations (RSDs) were less than 20%. The application of this method showed that target pesticides were screened out in 10 samples out of 21 practical samples, in which the banned pesticide chlorpyrifos were detected in 3 out of the 10 samples. FAU - Wang, Shou-Ying AU - Wang SY AD - Laboratory of Quality & Safety Risk Assessment for Aquatic products (Shanghai), Ministry of Agriculture and Rural Affairs, East China Sea Fisheries Research Institute, Shanghai 200090, China. magnolia7319@163.com. AD - College of Food Science & Technology, Shanghai Ocean University, Shanghai 201306, China. magnolia7319@163.com. FAU - Kong, Cong AU - Kong C AUID- ORCID: 0000-0001-8084-2133 AD - Laboratory of Quality & Safety Risk Assessment for Aquatic products (Shanghai), Ministry of Agriculture and Rural Affairs, East China Sea Fisheries Research Institute, Shanghai 200090, China. kongcong@gmail.com. AD - Key Laboratory of East China Sea Fishery Resources Exploitation, Ministry of Agriculture and Rural Affairs, East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China. kongcong@gmail.com. FAU - Chen, Qing-Ping AU - Chen QP AD - Laboratory of Quality & Safety Risk Assessment for Aquatic products (Shanghai), Ministry of Agriculture and Rural Affairs, East China Sea Fisheries Research Institute, Shanghai 200090, China. chenqp128@163.com. AD - College of Food Science & Technology, Shanghai Ocean University, Shanghai 201306, China. chenqp128@163.com. FAU - Yu, Hui-Juan AU - Yu HJ AD - Laboratory of Quality & Safety Risk Assessment for Aquatic products (Shanghai), Ministry of Agriculture and Rural Affairs, East China Sea Fisheries Research Institute, Shanghai 200090, China. xdyh-7@163.com. AD - Key Laboratory of East China Sea Fishery Resources Exploitation, Ministry of Agriculture and Rural Affairs, East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China. xdyh-7@163.com. LA - eng GR - 31701698/National Natural Science Foundation of China/ GR - T2016010401/Shanghai Agriculture Applied Technology Development Program/ GR - KF1910/Shanghai Key Laboratory of Forensic Medicine (Academy of Forensic Science)/ PT - Journal Article DEP - 20190917 PL - Switzerland TA - Molecules JT - Molecules (Basel, Switzerland) JID - 100964009 RN - 0 (Pesticides) SB - IM MH - Animals MH - *Chromatography, High Pressure Liquid/methods MH - Fisheries MH - *Fishes MH - Food Analysis/methods MH - Food Contamination/*analysis MH - Pesticides/*analysis MH - *Tandem Mass Spectrometry/methods PMC - PMC6767809 OTO - NOTNLM OT - fishery drugs OT - high-resolution orbitrap mass spectrometry OT - pesticide OT - screening COIS- The authors declare no conflict of interest. EDAT- 2019/09/20 06:00 MHDA- 2020/02/12 06:00 PMCR- 2019/09/17 CRDT- 2019/09/20 06:00 PHST- 2019/07/05 00:00 [received] PHST- 2019/09/10 00:00 [revised] PHST- 2019/09/12 00:00 [accepted] PHST- 2019/09/20 06:00 [entrez] PHST- 2019/09/20 06:00 [pubmed] PHST- 2020/02/12 06:00 [medline] PHST- 2019/09/17 00:00 [pmc-release] AID - molecules24183375 [pii] AID - molecules-24-03375 [pii] AID - 10.3390/molecules24183375 [doi] PST - epublish SO - Molecules. 2019 Sep 17;24(18):3375. doi: 10.3390/molecules24183375.