PMID- 31533251 OWN - NLM STAT- MEDLINE DCOM- 20200204 LR - 20200204 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 20 IP - 18 DP - 2019 Sep 17 TI - PGE(1)-Containing Protocols Generate Mature (Leukemia-Derived) Dendritic Cells Directly from Leukemic Whole Blood. LID - 10.3390/ijms20184590 [doi] LID - 4590 AB - Dendritic cells (DCs) and leukemia-derived DC (DC(leu)) are potent stimulators of various immunoreactive cells and they play a pivotal role in the (re-) activation of the immune system. As a potential treatment tool for patients with acute myeloid leukemia, we developed and analyzed two new PGE(1)-containing protocols (Pici-(PGE1), Kit M) to generate DC/DC(leu) ex vivo from leukemic peripheral blood mononuclear cells (PBMCs) or directly from leukemic whole blood (WB) to simulate physiological conditions. Pici-(PGE1) generated significantly higher amounts of DCs from leukemic and healthy PBMCs when compared to control and comparable amounts as the already established protocol Pici-(PGE2). The proportions of sufficient DC-generation were even higher after DC/DC(leu)-generation with Pici-(PGE1). With Kits, it was possible to generate DCs and DC(leu) directly from leukemic and healthy WB without induction of blast proliferation. The average amounts of generated DCs and DC(leu)-subgroups were comparable with all Kits. The PGE(1) containing Kit M generated significantly higher amounts of mature DCs when compared to the PGE(2)-containing Kit K and increased the anti-leukemic-activity. In summary PGE(1)-containing protocols were suitable for generating DC/DC(leu) from PBMCs as well as from WB, which reliably (re-) activated immunoreactive cells, improved the overall ex vivo anti-leukemic activity, and influenced cytokine-release-profiles. FAU - Amberger, Daniel Christoph AU - Amberger DC AD - Medical Department 3, Working-group: Immune-Modulation, University Hospital Munich, 81377 Munich, Germany. daniel.amberger@gmx.at. FAU - Doraneh-Gard, Fatemeh AU - Doraneh-Gard F AD - Medical Department 3, Working-group: Immune-Modulation, University Hospital Munich, 81377 Munich, Germany. nikoo_792000@yahoo.com. FAU - Gunsilius, Carina AU - Gunsilius C AD - Medical Department 3, Working-group: Immune-Modulation, University Hospital Munich, 81377 Munich, Germany. Carina_Gunsilius@web.de. FAU - Weinmann, Melanie AU - Weinmann M AD - Medical Department 3, Working-group: Immune-Modulation, University Hospital Munich, 81377 Munich, Germany. melli.weinmann@hotmail.de. FAU - Mobius, Sabine AU - Mobius S AD - Medical Department 3, Working-group: Immune-Modulation, University Hospital Munich, 81377 Munich, Germany. sabine.moebius@tum.de. FAU - Kugler, Christoph AU - Kugler C AD - Medical Department 3, Working-group: Immune-Modulation, University Hospital Munich, 81377 Munich, Germany. christophkugler@gmx.net. FAU - Rogers, Nicole AU - Rogers N AUID- ORCID: 0000-0002-7882-6334 AD - Medical Department 3, Working-group: Immune-Modulation, University Hospital Munich, 81377 Munich, Germany. nicimunrog@yahoo.de. FAU - Bock, Corinna AU - Bock C AD - Medical Department 3, Working-group: Immune-Modulation, University Hospital Munich, 81377 Munich, Germany. corinna.boeck@uk-erlangen.de. FAU - Kodel, Uwe AU - Kodel U AUID- ORCID: 0000-0002-2666-0731 AD - Department of Neurology, Klinikum Grosshadern, Ludwig-Maximilians-University, 81377 Munich, Germany. Uwe.Koedel@med.uni-muenchen.de. FAU - Werner, Jan-Ole AU - Werner JO AD - Department of Hematology and Oncology, University Hospital of Tuebingen, 72076 Tuebingen, Germany. Jan-ole.werner@klinikum-nuernberg.de. FAU - Kramer, Doris AU - Kramer D AD - Department for Hematology and Oncology, University Hospital of Oldenburg, 26133 Oldenburg, Germany. onko.med@bhv.ameos.de. FAU - Eiz-Vesper, Britta AU - Eiz-Vesper B AD - Institute for Transfusion Medicine, Hannover Medical School, 30625 Hannover, Germany. Eiz-Vesper.Britta@mh-hannover.de. FAU - Rank, Andreas AU - Rank A AD - Department of Hematology and Oncology, University Hospital of Augsburg, 86156 Augsburg, Germany. andreas.rank@klinikum-augsburg.de. FAU - Schmid, Christoph AU - Schmid C AD - Department of Hematology and Oncology, University Hospital of Augsburg, 86156 Augsburg, Germany. christoph.schmid@klinikum-augsburg.de. FAU - Schmetzer, Helga Maria AU - Schmetzer HM AD - Medical Department 3, Working-group: Immune-Modulation, University Hospital Munich, 81377 Munich, Germany. Helga.Schmetzer@med.uni-muenchen.de. LA - eng PT - Journal Article DEP - 20190917 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 0 (Biomarkers) RN - 0 (Cytokines) RN - 39325-01-4 (Picibanil) RN - F5TD010360 (Alprostadil) SB - IM MH - Adult MH - Aged MH - Alprostadil/*pharmacology MH - Biomarkers MH - Cell Differentiation/drug effects MH - Cytokines/metabolism MH - Dendritic Cells/*cytology/*drug effects MH - Female MH - Flow Cytometry MH - Humans MH - Immunomodulation/drug effects MH - Immunophenotyping MH - Leukemia, Myeloid, Acute/metabolism MH - Leukocytes, Mononuclear/drug effects/immunology/metabolism MH - Male MH - Middle Aged MH - Picibanil/pharmacology MH - Young Adult PMC - PMC6769744 OTO - NOTNLM OT - AML OT - PGE1 OT - dendritic cells OT - immunotherapy OT - leukemia-derived dendritic cells COIS- All authors declare, that there are no financial conflicts in regard to this work. EDAT- 2019/09/20 06:00 MHDA- 2020/02/06 06:00 PMCR- 2019/09/01 CRDT- 2019/09/20 06:00 PHST- 2019/07/24 00:00 [received] PHST- 2019/09/06 00:00 [revised] PHST- 2019/09/10 00:00 [accepted] PHST- 2019/09/20 06:00 [entrez] PHST- 2019/09/20 06:00 [pubmed] PHST- 2020/02/06 06:00 [medline] PHST- 2019/09/01 00:00 [pmc-release] AID - ijms20184590 [pii] AID - ijms-20-04590 [pii] AID - 10.3390/ijms20184590 [doi] PST - epublish SO - Int J Mol Sci. 2019 Sep 17;20(18):4590. doi: 10.3390/ijms20184590.