PMID- 31534842
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231013
IS  - 2167-8359 (Print)
IS  - 2167-8359 (Electronic)
IS  - 2167-8359 (Linking)
VI  - 7
DP  - 2019
TI  - Crucial lncRNAs associated with adipocyte differentiation from human 
      adipose-derived stem cells based on co-expression and ceRNA network analyses.
PG  - e7544
LID - 10.7717/peerj.7544 [doi]
LID - e7544
AB  - BACKGROUND: Injection of adipose-derived stem cells (ASCs) is a promising 
      treatment for facial contour deformities. However, its treatment mechanisms 
      remain largely unknown. The study aimed to explain the molecular mechanisms of 
      adipogenic differentiation from ASCs based on the roles of long noncoding RNAs 
      (lncRNAs). METHODS: Datasets of mRNA-lncRNA (GSE113253) and miRNA (GSE72429) 
      expression profiling were collected from Gene Expression Omnibus database. The 
      differentially expressed genes (DEGs), lncRNAs (DELs) and miRNAs (DEMs) between 
      undifferentiated and adipocyte differentiated human ASCs were identified using 
      the Linear Models for Microarray Data method. DELs related co-expression and 
      competing endogenous RNA (ceRNA) networks were constructed. Protein-protein 
      interaction (PPI) analysis was performed to screen crucial target genes. RESULTS: 
      A total of 748 DEGs, 17 DELs and 51 DEMs were identified. A total of 13 DELs and 
      279 DEGs with Pearson correlation coefficients > 0.9 and p-value < 0.01 were 
      selected to construct the co-expression network. A total of 151 interaction pairs 
      among 112 nodes (10 DEMs; eight DELs; 94 DEGs) were obtained to construct the 
      ceRNA network. By comparing the lncRNAs and mRNAs in two networks, five lncRNAs 
      (SNHG9, LINC02202, UBAC2-AS1, PTCSC3 and myocardial infarction associated 
      transcript (MIAT)) and 32 genes (i.e., such as phosphoinositide-3-kinase 
      regulatory subunit 1 (PIK3R1), protein tyrosine phosphatase receptor type B 
      (PTPRB)) were found to be shared. PPI analysis demonstrated PIK3R1 , forkhead box 
      O1 (FOXO1; a transcription factor) and estrogen receptor 1 (ESR1) were hub genes, 
      which could be regulated by the miRNAs that interacted with the above five 
      lncRNAs, such as LINC02202-miR-136-5p-PIK3R1, LINC02202-miR-381-3p-FOXO1 and 
      MIAT-miR-18a-5p-ESR1. LINC02202 also could directly co-express with PIK3R1. 
      Furthermore, PTPRB was predicted to be modulated by co-expression with LINC01119. 
      CONCLUSION: MIAT, LINC02202 and LINC01119 may be potentially important, new 
      lncRNAs associated with adipogenic differentiation of ASCs. They may be involved 
      in adipogenesis by acting as a ceRNA or co-expressing with their targets.
FAU - Chen, Kana
AU  - Chen K
AD  - Department of Plastic Surgery, Hwa Mei Hospital, University of Chinese Academy of 
      Sciences, Ningbo, Zhejiang, China.
FAU - Xie, Shujie
AU  - Xie S
AD  - Department of Hepatobiliary Surgery, Hwa Mei Hospital, University of Chinese 
      Academy of Sciences, Ningbo, Zhejiang, China.
FAU - Jin, Wujun
AU  - Jin W
AD  - Department of Plastic Surgery, Hwa Mei Hospital, University of Chinese Academy of 
      Sciences, Ningbo, Zhejiang, China.
LA  - eng
PT  - Journal Article
DEP - 20190906
PL  - United States
TA  - PeerJ
JT  - PeerJ
JID - 101603425
PMC - PMC6733242
OTO - NOTNLM
OT  - Adipogenic differentiation
OT  - Co-expression
OT  - Human adipose tissue-derived stromal stem cells
OT  - ceRNA
OT  - lncRNA
OT  - miRNA
COIS- The authors declare that they have no competing interests.
EDAT- 2019/09/20 06:00
MHDA- 2019/09/20 06:01
PMCR- 2019/09/06
CRDT- 2019/09/20 06:00
PHST- 2019/05/15 00:00 [received]
PHST- 2019/07/24 00:00 [accepted]
PHST- 2019/09/20 06:00 [entrez]
PHST- 2019/09/20 06:00 [pubmed]
PHST- 2019/09/20 06:01 [medline]
PHST- 2019/09/06 00:00 [pmc-release]
AID - 7544 [pii]
AID - 10.7717/peerj.7544 [doi]
PST - epublish
SO  - PeerJ. 2019 Sep 6;7:e7544. doi: 10.7717/peerj.7544. eCollection 2019.