PMID- 31535058
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220410
IS  - 2452-073X (Electronic)
IS  - 2452-073X (Linking)
VI  - 6
DP  - 2019 Aug-Dec
TI  - Monosodium iodoacetate-induced monoarthritis develops differently in knee versus 
      ankle joint in rats.
PG  - 100036
LID - 10.1016/j.ynpai.2019.100036 [doi]
LID - 100036
AB  - OBJECTIVE: Disability and movement-related pain are major symptoms of joint 
      disease, motivating the development of methods to quantify motor behaviour in 
      rodent joint pain models. We compared effects on behaviour, assessed the levels 
      of biochemical mediators and made a detailed histopathological evaluation after 
      induction of rat monoiodoacetate (MIA) monoarthritis into the ankle or knee 
      joint. DESIGN: Twenty-seven male Lewis rats were used. Before and up to 28 days 
      after induction, they were tested for weight bearing during walking (dynamic), 
      and standing (static), and for mechanical sensitivity. At termination synovial 
      fluid was taken from ankle and/or knee joints for analysis of monocyte 
      chemoattractant protein-1 (MCP-1), interleukin-6 (IL-6), macrophage inflammatory 
      protein 3 alpha (MIP-3alpha), keratinocyte chemoattractant (KC)/human 
      growth-regulated oncogene (GRO) and L(+)-lactate, and from separate rats joints 
      were collected for histopathological assessment. RESULTS: MIA ankle joint 
      injection gave a marked reduction of dynamic weight bearing during the first 
      days, not seen in rats with knee joint injection. At three weeks, it was 
      decreased in the group with knee injection, but not in those with ankle 
      injection. However, the different injection sites caused similar reductions in 
      static weight bearing during the early phase, which was normalized in the group 
      with ankle injection but continued and was strengthened with time in the knee 
      injected group. Histopathological assessment, biochemical mediators and joint 
      swelling confirmed the disparate profiles. CONCLUSIONS: This work shows that 
      ankle versus knee joint injection of MIA resulted in different profiles in rats, 
      which may mirror what has been found in human patients with osteoarthritis.
FAU - Angeby Moller, Kristina
AU  - Angeby Moller K
AD  - AstraZeneca R&D Sodertalje, CNSP iMed Science, SE-151 85 Sodertalje, Sweden.
AD  - Department of Physiology and Pharmacology, Karolinska Institutet, SE-171 76 
      Stockholm, Sweden.
FAU - Klein, Stephanie
AU  - Klein S
AD  - AstraZeneca R&D Sodertalje, Safety Pharmacology, SE-151 85 Sodertalje, Sweden.
FAU - Seeliger, Frank
AU  - Seeliger F
AD  - AstraZeneca R&D Sodertalje, Safety Pharmacology, SE-151 85 Sodertalje, Sweden.
FAU - Finn, Anja
AU  - Finn A
AD  - AstraZeneca R&D Sodertalje, CNSP iMed Science, SE-151 85 Sodertalje, Sweden.
AD  - Department of Physiology and Pharmacology, Karolinska Institutet, SE-171 76 
      Stockholm, Sweden.
FAU - Stenfors, Carina
AU  - Stenfors C
AD  - AstraZeneca R&D Sodertalje, CNSP iMed Science, SE-151 85 Sodertalje, Sweden.
FAU - Svensson, Camilla I
AU  - Svensson CI
AD  - Department of Physiology and Pharmacology, Karolinska Institutet, SE-171 76 
      Stockholm, Sweden.
LA  - eng
PT  - Journal Article
DEP - 20190903
PL  - United States
TA  - Neurobiol Pain
JT  - Neurobiology of pain (Cambridge, Mass.)
JID - 101705141
PMC - PMC6744596
OTO - NOTNLM
OT  - Arthritis
OT  - Gait analysis
OT  - Inflammation
OT  - Pain
OT  - PawPrint
OT  - Weight bearing
COIS- The authors declare that they have no known competing financial interests or 
      personal relationships that could have appeared to influence the work reported in 
      this paper
EDAT- 2019/09/20 06:00
MHDA- 2019/09/20 06:01
PMCR- 2019/09/03
CRDT- 2019/09/20 06:00
PHST- 2019/05/19 00:00 [received]
PHST- 2019/08/22 00:00 [revised]
PHST- 2019/08/29 00:00 [accepted]
PHST- 2019/09/20 06:00 [entrez]
PHST- 2019/09/20 06:00 [pubmed]
PHST- 2019/09/20 06:01 [medline]
PHST- 2019/09/03 00:00 [pmc-release]
AID - S2452-073X(19)30011-X [pii]
AID - 100036 [pii]
AID - 10.1016/j.ynpai.2019.100036 [doi]
PST - epublish
SO  - Neurobiol Pain. 2019 Sep 3;6:100036. doi: 10.1016/j.ynpai.2019.100036. 
      eCollection 2019 Aug-Dec.