PMID- 31535377
OWN - NLM
STAT- MEDLINE
DCOM- 20201216
LR  - 20201216
IS  - 1097-4652 (Electronic)
IS  - 0021-9541 (Linking)
VI  - 235
IP  - 3
DP  - 2020 Mar
TI  - Signaling pathways in cytoskeletal responses to plasma membrane depolarization in 
      corneal endothelial cells.
PG  - 2947-2962
LID - 10.1002/jcp.29200 [doi]
AB  - In previous work, we reported that plasma membrane potential depolarization 
      (PMPD) provokes cortical F-actin remodeling in bovine corneal endothelial (BCE) 
      cells in culture, which eventually leads to the appearance of intercellular gaps. 
      In kidney epithelial cells it has been shown that PMPD determines an 
      extracellular-signal-regulated kinase (ERK)/Rho-dependent increase in 
      diphosphorylated myosin light chain (ppMLC). The present study investigated the 
      signaling pathways involved in the response of BCE cells to PMPD. Differently to 
      renal epithelial cells, we observed that PMPD leads to a decrease in 
      monophosphorylated MLC (pMLC) without affecting diphosphorylated MLC. Also, that 
      the pMLC reduction is a consequence of cyclic adenosine 3',5'-monophosphate 
      (cAMP)/protein kinase A (PKA) activation. In addition, we found evidence that the 
      cAMP increase mostly depends on soluble adenylyl cyclase activity. Inhibition of 
      this enzyme reduces the effect of PMPD on the cAMP rise, F-actin remodeling, and 
      pMLC decrease. No changes in phosho-ERK were observed, although we could 
      determine that RhoA undergoes activation. Our results suggested that active RhoA 
      is not involved in the intercellular gap formation. Overall, the findings of this 
      study support the view that, differently to renal epithelial cells, in BCE cells 
      PMPD determines cytoskeletal reorganization via activation of the cAMP/PKA 
      pathway.
CI  - (c) 2019 Wiley Periodicals, Inc.
FAU - Evans, Frances
AU  - Evans F
AD  - Departamento de Histologia y Embriologia, Facultad de Medicina, Universidad de la 
      Republica, Montevideo, Uruguay.
FAU - Hernandez, Julio A
AU  - Hernandez JA
AD  - Seccion Biofisica, Facultad de Ciencias, Universidad de la Republica, Montevideo, 
      Uruguay.
FAU - Chifflet, Silvia
AU  - Chifflet S
AUID- ORCID: 0000-0003-4935-8591
AD  - Departamento de Bioquimica, Facultad de Medicina, Universidad de la Republica, 
      Montevideo, Uruguay.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190918
PL  - United States
TA  - J Cell Physiol
JT  - Journal of cellular physiology
JID - 0050222
RN  - 0 (Actins)
RN  - 0 (Myosin Light Chains)
RN  - E0399OZS9N (Cyclic AMP)
RN  - EC 2.7.11.1 (rho-Associated Kinases)
RN  - K72T3FS567 (Adenosine)
SB  - IM
MH  - Actins/metabolism
MH  - Adenosine/metabolism
MH  - Animals
MH  - Cattle
MH  - Cell Membrane/*metabolism
MH  - Cells, Cultured
MH  - Cyclic AMP/metabolism
MH  - Cytoskeleton/*metabolism
MH  - Endothelial Cells/*metabolism
MH  - Myosin Light Chains/metabolism
MH  - Phosphorylation/drug effects
MH  - Signal Transduction/*physiology
MH  - rho-Associated Kinases/metabolism
OTO - NOTNLM
OT  - actin cytoskeleton
OT  - cAMP/PKA
OT  - corneal endothelium
OT  - myosin light chain
OT  - plasma membrane depolarization
EDAT- 2019/09/20 06:00
MHDA- 2020/12/17 06:00
CRDT- 2019/09/20 06:00
PHST- 2018/12/16 00:00 [received]
PHST- 2019/08/26 00:00 [accepted]
PHST- 2019/09/20 06:00 [pubmed]
PHST- 2020/12/17 06:00 [medline]
PHST- 2019/09/20 06:00 [entrez]
AID - 10.1002/jcp.29200 [doi]
PST - ppublish
SO  - J Cell Physiol. 2020 Mar;235(3):2947-2962. doi: 10.1002/jcp.29200. Epub 2019 Sep 
      18.