PMID- 31537223
OWN - NLM
STAT- MEDLINE
DCOM- 20191017
LR  - 20191017
IS  - 2095-4352 (Print)
VI  - 31
IP  - 8
DP  - 2019 Aug
TI  - [Effect of overexpression of microRNA-21-5p on early apoptosis of type II 
      alveolar epithelial cells in rats with hyperoxic acute lung injury].
PG  - 978-982
LID - 10.3760/cma.j.issn.2095-4352.2019.08.013 [doi]
AB  - OBJECTIVE: To investigate the effect of overexpression of microRNA-21-5p 
      (miR-21-5p) on early apoptosis of type II alveolar epithelial cells (AEC II) in 
      rats with hyperoxic acute lung injury (HALI). METHODS: The Sprague-Dawley (SD) 
      rats were randomly divided into four groups: control group (CON group), hyperoxia 
      group (H group), overexpression group (OE group) and empty vector group (EV 
      group), with 20 rats in each group. HALI animal model was made by inhaling high 
      concentration oxygen (oxygen concentration >/= 90%); CON group was arranged to 
      inhale room air. The miR-21-5p adeno-associated virus-6 (AAV-6) overexpression 
      vectors or empty vectors were dripped into the lungs of OE group and EV group 
      through tracheal tube, respectively. The hyperoxia model was prepared after 3 
      weeks of feeding. At 0, 24, 48 and 60 hours after making model, 5 rats were 
      selected to detect lung injury parameters: oxygenation index (OI), respiratory 
      index (RI), wet/dry ratio (W/D), pathological injury score of lung tissue; 
      real-time quantitative polymerase chain reaction (RT-qPCR) was used to detect the 
      expression of miR-21-5p in AEC II, and flow cytometry was used to detect the 
      early apoptotic rate of AEC II. RESULTS: (1) The lung injury parameters: in H 
      group, the OI gradually decreased with time, but the RI, lung W/D ratio and 
      pathological score increased gradually with time, the difference between CON 
      group was statistically significant at 24 hours [OI (mmHg, 1 mmHg = 0.133 kPa): 
      336.04+/-5.79 vs. 400.22+/-19.70, RI: 0.20+/-0.02 vs. 0.10+/-0.06, lung W/D ratio: 
      5.04+/-0.09 vs. 4.85+/-0.09, lung tissue pathological score: 0.13+/-0.01 vs. 0.07+/-0.01, 
      all P < 0.05]. It indicated that HALI model could be successfully established by 
      inhaling high concentration oxygen continuously. (2) The expression of miR-21-5p: 
      the miR-21-5p was gradually increased in H, OE and EV groups, and the expression 
      of miR-21-5p was significantly higher than that in CON group at 24, 48 and 60 
      hours. Compared with H group, the expression of miR-21-5p was significantly 
      increased further in OE group at 0, 24, 48 and 60 hours (2(-DeltaDeltaCt): 3.75+/-0.11 vs. 
      0.98+/-0.14, 3.98+/-0.12 vs. 1.18+/-0.13, 4.28+/-0.18 vs. 1.49+/-0.06, 4.66+/-0.12 vs. 
      1.80+/-0.12, all P < 0.05). (3) The early apoptosis of AEC II: the early apoptosis 
      rate gradually increased with time in H, OE and EV groups, and the early 
      apoptosis of AEC II was significantly higher than that in CON group at 24, 48 and 
      60 hours. Compared with H group, the early apoptosis rate was significantly 
      decreased in OE group at 24, 48 and 60 hours [(1.22+/-0.63)% vs. (2.84+/-0.59)%, 
      (5.76+/-0.18)% vs. (13.10+/-2.01)%, (29.48+/-0.48)% vs. (49.04+/-1.36)%, all P < 0.05]. 
      (4) There was no significant difference in the expression of miR-21-5p and the 
      early apoptosis of AEC II cells between EV group and H group at each time point. 
      CONCLUSIONS: Overexpression of miR-21-5p could inhibit the early apoptosis of 
      AECII in rats with HALI.
FAU - Zhang, Wei
AU  - Zhang W
AD  - Department of Intensive Care Unit, Affiliated Hospital of Zunyi Medical College, 
      Zunyi 563003, Guizhou, China.
FAU - Xu, Le
AU  - Xu L
AD  - Department of Anesthesiology, Affiliated Hospital of Qingdao University, Qingdao 
      266003, Shandong, China. Corresponding author: Chen Miao, Email: 
      chenmiao64@163.com.
FAU - Chen, Miao
AU  - Chen M
AD  - Department of Intensive Care Unit, Affiliated Hospital of Zunyi Medical College, 
      Zunyi 563003, Guizhou, China.
FAU - Mei, Hong
AU  - Mei H
AD  - Department of Intensive Care Unit, Affiliated Hospital of Zunyi Medical College, 
      Zunyi 563003, Guizhou, China.
FAU - Li, Kang
AU  - Li K
AD  - Department of Intensive Care Unit, Affiliated Hospital of Zunyi Medical College, 
      Zunyi 563003, Guizhou, China.
FAU - Qin, Song
AU  - Qin S
AD  - Department of Intensive Care Unit, Affiliated Hospital of Zunyi Medical College, 
      Zunyi 563003, Guizhou, China.
FAU - Liu, Guoyue
AU  - Liu G
AD  - Department of Intensive Care Unit, Affiliated Hospital of Zunyi Medical College, 
      Zunyi 563003, Guizhou, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhonghua Wei Zhong Bing Ji Jiu Yi Xue
JT  - Zhonghua wei zhong bing ji jiu yi xue
JID - 101604552
RN  - 0 (MicroRNAs)
RN  - 0 (mirn21 microRNA, rat)
SB  - IM
MH  - *Acute Lung Injury
MH  - Alveolar Epithelial Cells
MH  - Animals
MH  - Apoptosis
MH  - *Hyperoxia
MH  - Lung
MH  - MicroRNAs/*metabolism
MH  - Random Allocation
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 2019/09/21 06:00
MHDA- 2019/10/18 06:00
CRDT- 2019/09/21 06:00
PHST- 2019/09/21 06:00 [entrez]
PHST- 2019/09/21 06:00 [pubmed]
PHST- 2019/10/18 06:00 [medline]
AID - 10.3760/cma.j.issn.2095-4352.2019.08.013 [doi]
PST - ppublish
SO  - Zhonghua Wei Zhong Bing Ji Jiu Yi Xue. 2019 Aug;31(8):978-982. doi: 
      10.3760/cma.j.issn.2095-4352.2019.08.013.