PMID- 31538845
OWN - NLM
STAT- MEDLINE
DCOM- 20200210
LR  - 20200210
IS  - 1532-4281 (Electronic)
IS  - 1079-9893 (Linking)
VI  - 39
IP  - 3
DP  - 2019 Jun
TI  - PPARgamma and mitophagy are involved in hypoxia/reoxygenation-induced renal tubular 
      epithelial cells injury.
PG  - 235-242
LID - 10.1080/10799893.2019.1660894 [doi]
AB  - Renal tubular epithelial cell (RTEC) injury is the main cause and common 
      pathological process of various renal diseases. Mitochondrial dysfunction (MtD) 
      is a pathological process after renal injury. Mitophagy is vital for 
      mitochondrial function. Hypoxia is a common cause of RTEC injury. Peroxisome 
      proliferator-activated receptor gamma (PPARgamma) is involved in cell proliferation, 
      apoptosis, and inflammation. Previous studies have shown that the low expression 
      of PPARgamma might be involved in hypoxia-induced RTEC injury. The present study 
      aimed to investigate the correlation between PPARgamma and mitophagy in damaged RTEC 
      in the hypoxia/reoxygenation (HR) model. The results showed that HR inhibited the 
      expression of PPARgamma, but increased the expression of LC3II, Atg5, SQSTM1/P62, and 
      PINK1 in a time-dependent manner. Moreover, mitochondrial DNA (mt DNA) copy 
      number, mitochondria membrane potential (MMP) levels, ATP content, and cell 
      viability were decreased in hypoxic RTECs, the expression of SQSTM1/P62 and 
      PINK1, the release of cytochrome c (cyt C), and production of reactive oxygen 
      species (ROS) were increased. Mitochondrial-containing autophagosomes (APs) were 
      detected using transmission election microscope (TEM) and laser scanning confocal 
      microscope (LSCM). Furthermore, PPARgamma protein expression was negatively 
      correlated with that of LC3II, PINK1, and the positive rate of RTEC-containing 
      mitochondrial-containing APs (all p < .05), but positively correlated with cell 
      viability, MMP level, and ATP content (all p < .05). These data suggested that 
      PPARgamma and mitophagy are involved in the RTEC injury process. Thus, a close 
      association could be detected between PPARgamma and mitophagy in HR-induced RTEC 
      injury, albeit additional investigation is imperative.
FAU - Wei, Luming
AU  - Wei L
AD  - Department of Pediatrics, Guangxi Medical University First Affiliated Hospital , 
      Nanning 530021 , China.
FAU - Qin, Yuanhan
AU  - Qin Y
AD  - Department of Pediatrics, Guangxi Medical University First Affiliated Hospital , 
      Nanning 530021 , China.
FAU - Jiang, Ling
AU  - Jiang L
AD  - Department of Pediatrics, Guangxi Medical University First Affiliated Hospital , 
      Nanning 530021 , China.
FAU - Yu, Xueyun
AU  - Yu X
AD  - Department of Pediatrics, Guangxi Medical University First Affiliated Hospital , 
      Nanning 530021 , China.
FAU - Xi, Zhiyang
AU  - Xi Z
AD  - Department of Pediatrics, Guangxi Medical University First Affiliated Hospital , 
      Nanning 530021 , China.
LA  - eng
PT  - Journal Article
DEP - 20190905
PL  - England
TA  - J Recept Signal Transduct Res
JT  - Journal of receptor and signal transduction research
JID - 9509432
RN  - 0 (PPAR gamma)
RN  - 0 (RNA, Messenger)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Animals
MH  - Autophagy
MH  - Cell Hypoxia
MH  - Cell Line
MH  - Cell Survival
MH  - Epithelial Cells/*pathology/ultrastructure
MH  - Kidney Tubules/*pathology
MH  - Mitochondria/metabolism/pathology/ultrastructure
MH  - *Mitophagy
MH  - Oxygen
MH  - PPAR gamma/genetics/*metabolism
MH  - RNA, Messenger/genetics/metabolism
MH  - Rats
OTO - NOTNLM
OT  - PPARgamma
OT  - hypoxia/reoxygenation
OT  - mitophagy
OT  - renal tubular epithelial cells
EDAT- 2019/09/21 06:00
MHDA- 2020/02/11 06:00
CRDT- 2019/09/21 06:00
PHST- 2019/09/21 06:00 [entrez]
PHST- 2019/09/21 06:00 [pubmed]
PHST- 2020/02/11 06:00 [medline]
AID - 10.1080/10799893.2019.1660894 [doi]
PST - ppublish
SO  - J Recept Signal Transduct Res. 2019 Jun;39(3):235-242. doi: 
      10.1080/10799893.2019.1660894. Epub 2019 Sep 5.