PMID- 31539353
OWN - NLM
STAT- MEDLINE
DCOM- 20200422
LR  - 20200422
IS  - 1868-1891 (Electronic)
IS  - 1868-1883 (Linking)
VI  - 40
IP  - 1
DP  - 2019 Sep 20
TI  - Cholecalciferol modulates the phenotype of differentiated monocyte-derived 
      dendritic cells without altering HIV-1 transfer to CD4+ T cells.
LID - /j/hmbci.2019.40.issue-1/hmbci-2019-0003/hmbci-2019-0003.xml [pii]
LID - 10.1515/hmbci-2019-0003 [doi]
AB  - Background Dendritic cells (DCs) play a crucial role during HIV-1 transmission 
      due to their ability to transfer virions to susceptible CD4+ T cells, 
      particularly in the lymph nodes during antigen presentation which favors the 
      establishment of systemic infection. As mature dendritic cells (mDCs) exhibit a 
      greater ability to transfer virions, compared to immature DCs (iDCs), maintenance 
      of an iDC phenotype could decrease viral transmission. The immunomodulatory 
      vitamin D (VitD) has been shown to reduce activation and maturation of DCs; 
      hence, we hypothesized that it would reduce viral transference by DCs. Materials 
      and methods We evaluated the effect of in vitro treatment with a precursor of 
      VitD, cholecalciferol, on the activation/maturation phenotype of differentiated 
      monocyte-derived DCs and their ability to transfer HIV-1 to autologous CD4+ T 
      cells. Results Our findings show that although cholecalciferol decreases the 
      activation of iDCs, it did not impact the maturation phenotype after LPS 
      treatment nor iDCs' ability to transfer viral particles to target cells. 
      Conclusion These findings suggest that despite cholecalciferol potentially 
      modulates the phenotype of mucosal iDCs in vivo, such modulation might not impact 
      the ability of these cells to transfer HIV-1 to target CD4+ T cells.
FAU - Gonzalez, Sandra M
AU  - Gonzalez SM
AD  - Grupo Inmunovirologia, Facultad de Medicina, Universidad de Antioquia UdeA, 
      Medellin, Colombia.
FAU - Aguilar-Jimenez, Wbeimar
AU  - Aguilar-Jimenez W
AD  - Grupo Inmunovirologia, Facultad de Medicina, Universidad de Antioquia UdeA, 
      Medellin, Colombia.
FAU - Alvarez, Natalia
AU  - Alvarez N
AD  - Grupo Inmunovirologia, Facultad de Medicina, Universidad de Antioquia UdeA, 
      Medellin, Colombia.
FAU - Rugeles, Maria T
AU  - Rugeles MT
AD  - Grupo Inmunovirologia, Facultad de Medicina, Universidad de Antioquia UdeA, Calle 
      70 No. 52-21, 050010 Medellin, Colombia, Phone: +57 4 2196551, Fax: 57 4 2196482.
LA  - eng
PT  - Journal Article
DEP - 20190920
PL  - Germany
TA  - Horm Mol Biol Clin Investig
JT  - Hormone molecular biology and clinical investigation
JID - 101538885
RN  - 0 (Immunologic Factors)
RN  - 0 (Vitamins)
RN  - 1C6V77QF41 (Cholecalciferol)
SB  - IM
MH  - CD4-Positive T-Lymphocytes/*drug effects/immunology/virology
MH  - Cells, Cultured
MH  - Cholecalciferol/*pharmacology
MH  - Dendritic Cells/*drug effects/immunology/virology
MH  - HIV Infections/*immunology/prevention & control/transmission/virology
MH  - HIV-1/*drug effects/immunology/physiology
MH  - Humans
MH  - Immunologic Factors/pharmacology
MH  - Monocytes/drug effects/immunology/virology
MH  - Virus Internalization/drug effects
MH  - Vitamins/*pharmacology
OTO - NOTNLM
OT  - DC phenotype
OT  - HIV-1 infection
OT  - HIV-1 transference
OT  - vitamin D
EDAT- 2019/09/21 06:00
MHDA- 2020/04/23 06:00
CRDT- 2019/09/21 06:00
PHST- 2019/02/11 00:00 [received]
PHST- 2019/06/20 00:00 [accepted]
PHST- 2019/09/21 06:00 [pubmed]
PHST- 2020/04/23 06:00 [medline]
PHST- 2019/09/21 06:00 [entrez]
AID - /j/hmbci.ahead-of-print/hmbci-2019-0003/hmbci-2019-0003.xml [pii]
AID - 10.1515/hmbci-2019-0003 [doi]
PST - epublish
SO  - Horm Mol Biol Clin Investig. 2019 Sep 
      20;40(1):/j/hmbci.2019.40.issue-1/hmbci-2019-0003/hmbci-2019-0003.xml. doi: 
      10.1515/hmbci-2019-0003.