PMID- 31539356
OWN - NLM
STAT- MEDLINE
DCOM- 20200422
LR  - 20200422
IS  - 1868-1891 (Electronic)
IS  - 1868-1883 (Linking)
VI  - 40
IP  - 2
DP  - 2019 Sep 20
TI  - Association between tumor necrosis factor-alpha gene-1031T/C promoter polymorphism 
      and endometriosis in a European population.
LID - /j/hmbci.2019.40.issue-2/hmbci-2019-0033/hmbci-2019-0033.xml [pii]
LID - 10.1515/hmbci-2019-0033 [doi]
AB  - Background Tumor necrosis factor-alpha (TNF-alpha) is a pro-inflammatory cytokine 
      which plays an important role in the pathogenesis of many diseases. Endometriosis 
      is one of the most common gynecological diseases. The purpose of this study was 
      to investigate the association of TNF-alpha-1031T/C polymorphism with the genetic 
      susceptibility of endometriosis in a European population. Materials and methods 
      In this case-control study, 51 endometriosis patients and 67 healthy control 
      women participated. We used endometrial tissue from the patients and peripheral 
      blood from the healthy women to extract DNA. Polymerase chain reaction (PCR) 
      analysis and the restriction enzyme Bbs I were used to analyze the -1031 T/C 
      polymorphism in the TNF-alpha gene promoter region. Statistical analysis was 
      performed using Fisher's exact test. We also calculated the odds ratios. Results 
      In the group of patients, 66.7% of women were detected with the TT genotype, 
      33.3% with the TC genotype and 0% with the CC genotype while in the control 
      group, 46.3% had the TT genotype, 47.8% had the TC genotype and 6% had the CC 
      genotype. There was a significant association between the TT genotype with 
      endometriosis (p = 0.03). There was no significant deviation from the 
      Hardy-Weinberg equilibrium. Conclusions The TC and CC genotypes appeared more 
      often in the healthy women than the endometriosis patients and this shows that 
      the C allele might have a protective role in endometriosis in the Greek 
      population. Further studies are needed to specify the role of this polymorphism 
      in pathogenesis of endometriosis and the mechanisms that protect the patients 
      from the disease.
FAU - Drakou, Anastasia
AU  - Drakou A
AUID- ORCID: 0000-0003-3992-2902
AD  - Sodertalje Hospital, Department of Obstetrics and Gynecology, 
      Tradgardsmastarstigen 10, 14432 Ronninge, Sweden.
AD  - Molecular Biology Unit, Division of Human Reproduction, 1st Department of 
      Obstetrics and Gynecology, National and Kapodistrian University of Athens, 
      Athens, Greece.
FAU - Mavrogianni, Despoina
AU  - Mavrogianni D
AD  - Molecular Biology Unit, Division of Human Reproduction, 1st Department of 
      Obstetrics and Gynecology, National and Kapodistrian University of Athens, 
      Athens, Greece.
FAU - Ntzeros, Konstantinos
AU  - Ntzeros K
AD  - 1st Department of Obstetrics and Gynecology, National and Kapodistrian University 
      of Athens, Athens, Greece.
FAU - Protopapas, Athanasios
AU  - Protopapas A
AD  - 1st Department of Obstetrics and Gynecology, National and Kapodistrian University 
      of Athens, Athens, Greece.
FAU - Drakakis, Petros
AU  - Drakakis P
AD  - Molecular Biology Unit, Division of Human Reproduction, 1st Department of 
      Obstetrics and Gynecology, National and Kapodistrian University of Athens, 
      Athens, Greece.
AD  - 1st Department of Obstetrics and Gynecology, National and Kapodistrian University 
      of Athens, Athens, Greece.
FAU - Loutradis, Dimitrios
AU  - Loutradis D
AD  - 1st Department of Obstetrics and Gynecology, National and Kapodistrian University 
      of Athens, Athens, Greece.
LA  - eng
PT  - Journal Article
DEP - 20190920
PL  - Germany
TA  - Horm Mol Biol Clin Investig
JT  - Hormone molecular biology and clinical investigation
JID - 101538885
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Adult
MH  - Case-Control Studies
MH  - Endometriosis/epidemiology/*genetics
MH  - Female
MH  - Gene Frequency
MH  - Genetic Predisposition to Disease/epidemiology/genetics
MH  - Greece/epidemiology
MH  - Humans
MH  - *Polymorphism, Single Nucleotide
MH  - Promoter Regions, Genetic
MH  - Protective Factors
MH  - Tumor Necrosis Factor-alpha/*genetics
OTO - NOTNLM
OT  - endometriosis
OT  - polymorphism
OT  - promoter
OT  - tumor necrosis factor-alpha
EDAT- 2019/09/21 06:00
MHDA- 2020/04/23 06:00
CRDT- 2019/09/21 06:00
PHST- 2019/06/22 00:00 [received]
PHST- 2019/08/18 00:00 [accepted]
PHST- 2019/09/21 06:00 [pubmed]
PHST- 2020/04/23 06:00 [medline]
PHST- 2019/09/21 06:00 [entrez]
AID - /j/hmbci.ahead-of-print/hmbci-2019-0033/hmbci-2019-0033.xml [pii]
AID - 10.1515/hmbci-2019-0033 [doi]
PST - epublish
SO  - Horm Mol Biol Clin Investig. 2019 Sep 
      20;40(2):/j/hmbci.2019.40.issue-2/hmbci-2019-0033/hmbci-2019-0033.xml. doi: 
      10.1515/hmbci-2019-0033.