PMID- 31539364
OWN - NLM
STAT- MEDLINE
DCOM- 20200501
LR  - 20260314
IS  - 1748-605X (Electronic)
IS  - 1748-6041 (Linking)
VI  - 14
IP  - 6
DP  - 2019 Sep 20
TI  - Nanocomposite-coated porous templates for engineered bone scaffolds: a parametric 
      study of layer-by-layer assembly conditions.
PG  - 065008
LID - 10.1088/1748-605X/ab3b7b [doi]
AB  - Using the layer-by-layer (LbL) assembly technique to deposit mechanically 
      reinforcing coatings onto porous templates is a route for fabricating engineered 
      bone scaffold materials with a combination of high porosity, strength, and 
      stiffness. LbL assembly involves the sequential deposition of nano- to 
      micro-scale multilayer coatings from aqueous solutions. Here, a design of 
      experiments (DOE) approach was used to evaluate LbL assembly of polyethyleneimine 
      (PEI), polyacrylic acid (PAA), and nanoclay coatings onto open-cell polyurethane 
      foam templates. The thickness of the coatings, and the porosity, elastic modulus 
      and collapse stress of coated foam templates were most strongly affected by the 
      pH of PAA solutions, salt concentration, and interactions between these factors. 
      The mechanical properties of coated foams correlated with the thickness of the 
      coatings, but were also ascribed to changes in the coating properties due to the 
      different assembly conditions. A DOE optimization aimed to balance the trade-off 
      between higher mechanical properties but lower porosity of foam templates with 
      increasing coating thickness. Micromechanical modeling predicted that deposition 
      of 116 QLs would achieve mechanical properties of cancellous bone (>0.05 GPa 
      stiffness and >2 MPa strength) at a suitable porosity of >70%. When capped with a 
      final layer of PAA and cross-linked via thermal treatment, the 
      PEI/PAA/PEI/nanoclay coatings exhibited good indirect cytotoxicity with 
      mesenchymal stem cells. The ability of LbL assembly to deposit a wide range of 
      functional constituents within multilayer-structured coatings makes the general 
      strategy of templated LbL assembly a powerful route for fabricating engineered 
      tissue scaffolds that can be applied onto various porous template materials to 
      achieve a wide range of properties, pore structures, and multifunctionality.
FAU - Ziminska, Monika
AU  - Ziminska M
AD  - School of Mechanical & Aerospace Engineering, Queen's University Belfast, BT9 
      5AH, United Kingdom.
FAU - Chalanqui, Marine J
AU  - Chalanqui MJ
AD  - School of Pharmacy, Queen's University Belfast, 97 Lisburn Road, Belfast BT9 7BL, 
      United Kingdom.
FAU - Chambers, Philip
AU  - Chambers P
AD  - School of Pharmacy, Queen's University Belfast, 97 Lisburn Road, Belfast BT9 7BL, 
      United Kingdom.
FAU - Acheson, Jonathan G
AU  - Acheson JG
AUID- ORCID: 0000-0003-3622-552X
AD  - School of Mechanical & Aerospace Engineering, Queen's University Belfast, BT9 
      5AH, United Kingdom.
FAU - McCarthy, Helen O
AU  - McCarthy HO
AD  - School of Pharmacy, Queen's University Belfast, 97 Lisburn Road, Belfast BT9 7BL, 
      United Kingdom.
FAU - Dunne, Nicholas J
AU  - Dunne NJ
AD  - School of Mechanical and Manufacturing Engineering, Dublin City University, 
      Dublin, Ireland.
AD  - Centre for Medical Engineering Research, School of Mechanical and Manufacturing 
      Engineering, Dublin City University, Dublin, Ireland.
AD  - School of Pharmacy, Queen's University Belfast, 97 Lisburn Road, Belfast BT9 7BL, 
      United Kingdom.
AD  - Trinity Centre for Bioengineering, Trinity Biomedical Sciences Institute, Trinity 
      College Dublin, Dublin, Ireland.
AD  - Department of Mechanical and Manufacturing Engineering, School of Engineering, 
      Trinity College Dublin, Dublin, Ireland.
AD  - Advanced Materials and Bioengineering Research Centre (AMBER), Royal College of 
      Surgeons in Ireland and Trinity College Dublin, Dublin, Ireland.
FAU - Hamilton, Andrew R
AU  - Hamilton AR
AUID- ORCID: 0000-0003-4627-849X
AD  - Engineering and the Environment, University of Southampton, Southampton SO17 1BJ, 
      United Kingdom.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190920
PL  - England
TA  - Biomed Mater
JT  - Biomedical materials (Bristol, England)
JID - 101285195
RN  - 0 (Acrylic Resins)
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Coated Materials, Biocompatible)
RN  - 0 (Cross-Linking Reagents)
RN  - 4Q93RCW27E (carbopol 940)
RN  - 9002-98-6 (Polyethyleneimine)
RN  - D1JT611TNE (Titanium)
SB  - IM
MH  - Acrylic Resins/chemistry
MH  - Animals
MH  - Anti-Bacterial Agents/chemistry
MH  - Biomimetic Materials/chemistry
MH  - Bone Marrow Cells/cytology
MH  - Bone and Bones/*physiology
MH  - Coated Materials, Biocompatible/chemistry
MH  - Compressive Strength
MH  - Cross-Linking Reagents/chemistry
MH  - Elasticity
MH  - Hydrogen-Ion Concentration
MH  - Materials Testing
MH  - Mesenchymal Stem Cells/cytology
MH  - Nanocomposites/*chemistry
MH  - Polyethyleneimine/chemistry
MH  - Porosity
MH  - Stress, Mechanical
MH  - Swine
MH  - Tissue Engineering/*methods
MH  - Tissue Scaffolds/chemistry
MH  - Titanium/chemistry
MH  - X-Ray Microtomography
EDAT- 2019/09/21 06:00
MHDA- 2020/05/02 06:00
CRDT- 2019/09/21 06:00
PHST- 2019/09/21 06:00 [entrez]
PHST- 2019/09/21 06:00 [pubmed]
PHST- 2020/05/02 06:00 [medline]
AID - 10.1088/1748-605X/ab3b7b [doi]
PST - epublish
SO  - Biomed Mater. 2019 Sep 20;14(6):065008. doi: 10.1088/1748-605X/ab3b7b.