PMID- 31540036
OWN - NLM
STAT- MEDLINE
DCOM- 20200714
LR  - 20200714
IS  - 2218-273X (Electronic)
IS  - 2218-273X (Linking)
VI  - 9
IP  - 9
DP  - 2019 Sep 14
TI  - Detection and identification of allergens from Canadian mustard varieties of 
      Sinapis alba and Brassica juncea.
LID - 10.3390/biom9090489 [doi]
LID - 489
AB  - Currently, information on the allergens profiles of different mustard varieties 
      is rather scarce. Therefore, the objective of this study was to assess protein 
      profiles and immunoglobulin E (IgE)-binding patterns of selected Canadian mustard 
      varieties. Optimization of a non-denaturing protein extraction from the seeds of 
      selected mustard varieties was first undertaken, and the various extracts were 
      quantitatively and qualitatively analyzed by means of protein recovery 
      determination and protein profiling. The IgE-binding patterns of selected mustard 
      seeds extracts were assessed by immunoblotting using sera from mustard sensitized 
      and allergic individuals. In addition to the known mustard allergens-Sin a 2 (11S 
      globulins), Sin a 1, and Bra j 1 (2S albumins)-the presence of other new 
      IgE-binding protein bands was revealed from both Sinapis alba and Brassica juncea 
      varieties. Mass spectrometry (MS) analysis of the in-gel digested IgE-reactive 
      bands identified the unknown ones as being oleosin, beta-glucosidase, enolase, and 
      glutathione-S transferase proteins. A bioinformatic comparison of the amino acid 
      sequence of the new IgE-binding mustard proteins with those of know allergens 
      revealed a number of strong homologies that are highly relevant for potential 
      allergic cross-reactivity. Moreover, it was found that Sin a 1, Bra j 1, and 
      cruciferin polypeptides exhibited a stronger IgE reactivity under non-reducing 
      conditions in comparison to reducing conditions, demonstrating the recognition of 
      conformational epitopes. These results further support the utilization of 
      non-denaturing extraction and analysis conditions, as denaturing conditions may 
      lead to failure in the detection of important immunoreactive epitopes.
FAU - L'Hocine, Lamia
AU  - L'Hocine L
AD  - Saint-Hyacinthe Research and Development Centre, Agriculture and Agri-Food 
      Canada, 3600 Casavant Blvd. W. Saint-Hyacinthe, QC, J2S 8E3, Canada. 
      lamia.lhocine@canada.ca.
FAU - Pitre, Melanie
AU  - Pitre M
AD  - Saint-Hyacinthe Research and Development Centre, Agriculture and Agri-Food 
      Canada, 3600 Casavant Blvd. W. Saint-Hyacinthe, QC, J2S 8E3, Canada. 
      melanie.pitre@canada.ca.
FAU - Achouri, Allaoua
AU  - Achouri A
AD  - Saint-Hyacinthe Research and Development Centre, Agriculture and Agri-Food 
      Canada, 3600 Casavant Blvd. W. Saint-Hyacinthe, QC, J2S 8E3, Canada. 
      allaoua.achouri@canada.ca.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190914
PL  - Switzerland
TA  - Biomolecules
JT  - Biomolecules
JID - 101596414
RN  - 0 (Allergens)
RN  - 0 (Antigens, Plant)
RN  - 0 (Plant Proteins)
RN  - 0 (Sin a 2 protein, Sinapis alba)
RN  - 0 (Sin a I protein, Sinapis alba)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Allergens/immunology/*isolation & purification
MH  - Antigens, Plant/immunology/isolation & purification
MH  - Canada
MH  - Humans
MH  - Immunoglobulin E/*metabolism
MH  - Mass Spectrometry
MH  - Mustard Plant/immunology/*metabolism
MH  - Plant Proteins/immunology/*isolation & purification
MH  - Seeds/immunology/metabolism
MH  - Sequence Analysis, Protein
MH  - Sinapis/immunology/*metabolism
PMC - PMC6770452
OTO - NOTNLM
OT  - Brassica juncea
OT  - IgE-binding
OT  - Sinapis alba
OT  - allergens
OT  - immunoblotting
OT  - mass spectrometry
OT  - mustard
OT  - protein extraction
OT  - sequence alignment
COIS- The authors declare no conflict of interest.
EDAT- 2019/09/22 06:00
MHDA- 2020/07/15 06:00
PMCR- 2019/09/01
CRDT- 2019/09/22 06:00
PHST- 2019/07/23 00:00 [received]
PHST- 2019/08/30 00:00 [revised]
PHST- 2019/09/10 00:00 [accepted]
PHST- 2019/09/22 06:00 [entrez]
PHST- 2019/09/22 06:00 [pubmed]
PHST- 2020/07/15 06:00 [medline]
PHST- 2019/09/01 00:00 [pmc-release]
AID - biom9090489 [pii]
AID - biomolecules-09-00489 [pii]
AID - 10.3390/biom9090489 [doi]
PST - epublish
SO  - Biomolecules. 2019 Sep 14;9(9):489. doi: 10.3390/biom9090489.