PMID- 31540161
OWN - NLM
STAT- MEDLINE
DCOM- 20200226
LR  - 20231014
IS  - 1420-3049 (Electronic)
IS  - 1420-3049 (Linking)
VI  - 24
IP  - 18
DP  - 2019 Sep 15
TI  - Improved Production and In Situ Recovery of Sesquiterpene (+)-Zizaene from 
      Metabolically-Engineered E. coli.
LID - 10.3390/molecules24183356 [doi]
LID - 3356
AB  - The sesquiterpene (+)-zizaene is the direct precursor of khusimol, the main 
      fragrant compound of the vetiver essential oil from Chrysopogon zizanioides and 
      used in nearly 20% of men's fine perfumery. The biotechnological production of 
      such fragrant sesquiterpenes is a promising alternative towards sustainability; 
      nevertheless, product recovery from fermentation is one of the main constraints. 
      In an effort to improve the (+)-zizaene recovery from a metabolically-engineered 
      Escherichia coli, we developed an integrated bioprocess by coupling fermentation 
      and (+)-zizaene recovery using adsorber extractants. Initially, (+)-zizaene 
      volatilization was confirmed from cultivations with no extractants but 
      application of liquid-liquid phase partitioning cultivation (LLPPC) improved 
      (+)-zizaene recovery nearly 4-fold. Furthermore, solid-liquid phase partitioning 
      cultivation (SLPPC) was evaluated by screening polymeric adsorbers, where Diaion 
      HP20 reached the highest recovery. Bioprocess was scaled up to 2 L bioreactors 
      and in situ recovery configurations integrated to fermentation were evaluated. 
      External recovery configuration was performed with an expanded bed adsorption 
      column and improved (+)-zizaene titers 2.5-fold higher than LLPPC. Moreover, 
      internal recovery configuration (IRC) further enhanced the (+)-zizaene titers 
      2.2-fold, whereas adsorption velocity was determined as critical parameter for 
      recovery efficiency. Consequently, IRC improved the (+)-zizaene titer 8.4-fold 
      and productivity 3-fold from our last report, achieving a (+)-zizaene titer of 
      211.13 mg L(-1) and productivity of 3.2 mg L(-1) h(-1). This study provides 
      further knowledge for integration of terpene bioprocesses by in situ product 
      recovery, which could be applied for many terpene studies towards the 
      industrialization of fragrant molecules.
FAU - Aguilar, Francisco
AU  - Aguilar F
AUID- ORCID: 0000-0002-6696-1993
AD  - Institute of Technical Chemistry, Leibniz University of Hannover, Callinstr. 5, 
      30167 Hannover, Germany. aguilar@iftc.uni-hannover.de.
FAU - Scheper, Thomas
AU  - Scheper T
AD  - Institute of Technical Chemistry, Leibniz University of Hannover, Callinstr. 5, 
      30167 Hannover, Germany. scheper@iftc.uni-hannover.de.
FAU - Beutel, Sascha
AU  - Beutel S
AD  - Institute of Technical Chemistry, Leibniz University of Hannover, Callinstr. 5, 
      30167 Hannover, Germany. beutel@iftc.uni-hannover.de.
LA  - eng
GR  - PED-058-2015-1/PINN program from the Ministry of Science, Technology and 
      Telecommunications of Costa Rica (MICITT)/
PT  - Journal Article
DEP - 20190915
PL  - Switzerland
TA  - Molecules
JT  - Molecules (Basel, Switzerland)
JID - 100964009
RN  - 0 (Oils, Volatile)
RN  - 0 (Polycyclic Sesquiterpenes)
RN  - 16223-63-5 (khusimol)
SB  - IM
MH  - Adsorption
MH  - Bioreactors
MH  - Chrysopogon/chemistry
MH  - Efficiency
MH  - Escherichia coli/*genetics/metabolism
MH  - Fermentation
MH  - Industrial Microbiology
MH  - Metabolic Engineering
MH  - Oils, Volatile/*chemistry
MH  - Polycyclic Sesquiterpenes/isolation & purification/*metabolism
MH  - Volatilization
PMC - PMC6767195
OTO - NOTNLM
OT  - (+)-zizaene
OT  - Chrysopogon zizanioides
OT  - expanded bed adsorption
OT  - in situ product recovery
OT  - khusimene
OT  - khusimol
OT  - sesquiterpenes
OT  - terpenes
OT  - vetiver essential oil
COIS- The authors declare no conflicts of interest. The funders had no role in the 
      design of the study; in the collection, analyses, or interpretation of data; in 
      the writing of the manuscript, or in the decision to publish the results.
EDAT- 2019/09/22 06:00
MHDA- 2020/02/27 06:00
PMCR- 2019/09/15
CRDT- 2019/09/22 06:00
PHST- 2019/08/17 00:00 [received]
PHST- 2019/09/12 00:00 [revised]
PHST- 2019/09/14 00:00 [accepted]
PHST- 2019/09/22 06:00 [entrez]
PHST- 2019/09/22 06:00 [pubmed]
PHST- 2020/02/27 06:00 [medline]
PHST- 2019/09/15 00:00 [pmc-release]
AID - molecules24183356 [pii]
AID - molecules-24-03356 [pii]
AID - 10.3390/molecules24183356 [doi]
PST - epublish
SO  - Molecules. 2019 Sep 15;24(18):3356. doi: 10.3390/molecules24183356.