PMID- 31541516
OWN - NLM
STAT- MEDLINE
DCOM- 20191231
LR  - 20201101
IS  - 1601-0825 (Electronic)
IS  - 1354-523X (Print)
IS  - 1354-523X (Linking)
VI  - 25
IP  - 8
DP  - 2019 Nov
TI  - Influence of T2DM and prediabetes on blood DC subsets and function in subjects 
      with periodontitis.
PG  - 2020-2029
LID - 10.1111/odi.13200 [doi]
AB  - OBJECTIVE: To compare the myeloid and plasmacytoid DC counts and maturation 
      status among subjects with/without generalized periodontitis (GP) and type 2 
      diabetes mellitus (T2DM). METHODS: The frequency and maturation status of myeloid 
      and plasmacytoid blood DCs were analyzed by flow cytometry in four groups of 15 
      subjects: healthy controls, T2DM with generalized CP (T2DM + GP), prediabetes 
      with GP (PD + GP), and normoglycemics with GP (NG + GP). RT-PCR was used to 
      determine levels of Porphyromonas gingivalis in the oral biofilms and within 
      panDCs. The role of exogenous glucose effects on differentiation and apoptosis of 
      healthy human MoDCs was explored in vitro. RESULTS: Relative to controls and to 
      NG + GP, T2DM + GP showed significantly lower CD1c + and CD303 + DC counts, while 
      CD141 + DCs were lower in T2DM + GP relative to controls. Blood DC maturation 
      required for mobilization and immune responsiveness was not observed. A 
      statistically significant trend was observed for P. gingivalis levels in the 
      biofilms of groups as follows: controls <NG+GP < PD+GP < T2DM+GP. Moreover, 
      significantly higher P. gingivalis levels were observed in blood DCs of NG + GP 
      than controls, whereas no differences were observed between controls and 
      PD + GP/T2DM + GP. In vitro differentiation of MoDCs was significantly decreased, 
      and apoptosis was increased by physiologically relevant glucose levels. 
      CONCLUSION: Type 2 diabetes mellitus appears to inhibit important DC immune 
      homeostatic functions, including expansion and bacterial scavenging, which might 
      be mediated by hyperglycemia.
CI  - (c) 2019 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd. All rights 
      reserved.
FAU - Rabelo, Mariana de Sousa
AU  - Rabelo MS
AUID- ORCID: 0000-0003-1385-8471
AD  - Division of Periodontics, Department of Stomatology, School of Dentistry, 
      University of Sao Paulo, Sao Paulo, Brazil.
AD  - Department of Periodontics, The Dental College of Georgia, Augusta University, 
      Augusta, GA, USA.
FAU - El-Awady, Ahmed
AU  - El-Awady A
AD  - Department of Periodontics, The Dental College of Georgia, Augusta University, 
      Augusta, GA, USA.
FAU - Moura Foz, Adriana
AU  - Moura Foz A
AD  - Division of Periodontics, Department of Stomatology, School of Dentistry, 
      University of Sao Paulo, Sao Paulo, Brazil.
FAU - Hisse Gomes, Giovane
AU  - Hisse Gomes G
AD  - Division of Periodontics, Department of Stomatology, School of Dentistry, 
      University of Sao Paulo, Sao Paulo, Brazil.
FAU - Rajendran, Mythilpriya
AU  - Rajendran M
AD  - Department of Periodontics, The Dental College of Georgia, Augusta University, 
      Augusta, GA, USA.
FAU - Meghil, Mohamed M
AU  - Meghil MM
AUID- ORCID: 0000-0003-2344-3579
AD  - Department of Periodontics, The Dental College of Georgia, Augusta University, 
      Augusta, GA, USA.
FAU - Lowry, Scott
AU  - Lowry S
AD  - Department of Periodontics, The Dental College of Georgia, Augusta University, 
      Augusta, GA, USA.
FAU - Romito, Giuseppe Alexandre
AU  - Romito GA
AUID- ORCID: 0000-0001-5669-912X
AD  - Division of Periodontics, Department of Stomatology, School of Dentistry, 
      University of Sao Paulo, Sao Paulo, Brazil.
FAU - Cutler, Christopher W
AU  - Cutler CW
AD  - Department of Periodontics, The Dental College of Georgia, Augusta University, 
      Augusta, GA, USA.
FAU - Susin, Cristiano
AU  - Susin C
AD  - Department of Periodontology, Adams School of Dentistry, University of North 
      Carolina at Chapel Hill, Chapel Hill, NC, USA.
LA  - eng
GR  - R01 DE014328/DE/NIDCR NIH HHS/United States
GR  - R21 DE020916/DE/NIDCR NIH HHS/United States
GR  - R01 DE014328 & R21 DE020916/Foundation for the National Institutes of Health/
GR  - 2011/06982-4/Fundacao de Amparo a Pesquisa do Estado de Sao Paulo/
PT  - Journal Article
DEP - 20191011
PL  - Denmark
TA  - Oral Dis
JT  - Oral diseases
JID - 9508565
MH  - Adult
MH  - Aged
MH  - Brazil
MH  - Dendritic Cells/*immunology
MH  - *Diabetes Mellitus, Type 2
MH  - Humans
MH  - Middle Aged
MH  - *Periodontitis
MH  - Porphyromonas gingivalis
MH  - *Prediabetic State
PMC - PMC6933074
MID - NIHMS1051264
OTO - NOTNLM
OT  - porphyromonas gingivalis
OT  - blood dendritic cells
OT  - periodontitis
OT  - prediabetes
OT  - type 2 diabetes
COIS- Conflicts of interest: none to declare.
EDAT- 2019/09/22 06:00
MHDA- 2020/01/01 06:00
PMCR- 2020/11/01
CRDT- 2019/09/22 06:00
PHST- 2018/10/29 00:00 [received]
PHST- 2019/09/06 00:00 [revised]
PHST- 2019/09/13 00:00 [accepted]
PHST- 2019/09/22 06:00 [pubmed]
PHST- 2020/01/01 06:00 [medline]
PHST- 2019/09/22 06:00 [entrez]
PHST- 2020/11/01 00:00 [pmc-release]
AID - 10.1111/odi.13200 [doi]
PST - ppublish
SO  - Oral Dis. 2019 Nov;25(8):2020-2029. doi: 10.1111/odi.13200. Epub 2019 Oct 11.