PMID- 31542554
OWN - NLM
STAT- MEDLINE
DCOM- 20210209
LR  - 20210209
IS  - 2213-7173 (Electronic)
IS  - 2213-7165 (Linking)
VI  - 20
DP  - 2020 Mar
TI  - High-level aminoglycoside resistance and distribution of aminoglycoside 
      resistance genes among Enterococcus spp. clinical isolates in Tehran, Iran.
PG  - 318-323
LID - S2213-7165(19)30206-1 [pii]
LID - 10.1016/j.jgar.2019.08.008 [doi]
AB  - OBJECTIVES: Enterococci have gained attention during the past decade as important 
      nosocomial pathogens. Their increasing prevalence has been paralleled by the 
      occurrence of multidrug-resistant and high-level aminoglycoside-resistant 
      strains. This study isolated Enterococcus spp. from hospital samples and 
      determined their antibiotic resistance profile, focusing on aminoglycosides, and 
      associated resistance mechanisms. METHODS: A total of 195 enterococci from 
      hospital samples in Tehran were studied. Isolates were identified by biochemical 
      reactions. Antimicrobial resistance was determined by disk diffusion. The 
      vancomycin MIC for vancomycin-resistant isolates was determined by agar dilution. 
      Detection of aminoglycoside resistance genes and intI1 and intI2 gene was 
      performed by PCR. RESULTS: The majority of isolates were Enterococcus faecalis 
      (65.1%), followed by Enterococcus faecium (31.8%), Enterococcus gallinarum (2.6%) 
      and Enterococcus solitarius (0.5%). According to antibiogram results, 42.1% of 
      isolates were high-level gentamicin-resistant (HLGR) and 40.5% were high-level 
      streptomycin-resistant (HLSR). There was a high prevalence of 
      aac(6')-Ie-aph(2")-Ia (96.3%) among HLGR isolates. ant(6)-Ia and aadA were 
      identified in 93.7% and 64.6% of HLSR isolates, respectively. aph(2'')-Ic was 
      detected in 7 isolates (3.6%) and aph(2'')-Ib in only 4 isolates (2.1%); no 
      isolates harboured aph(2'')-Id, intI1 or intI2. CONCLUSION: Multidrug resistance 
      was higher among HLGR and HLSR isolates compared with non-HLGR and non-HLSR 
      isolates, which may result in limited treatment options. More than 50% of 
      isolates were susceptible to aminoglycosides, thus correct identification in 
      clinical laboratories and administration of these antibiotics can result in 
      decreased used of antibiotics such as vancomycin and linezolid and help to reduce 
      the emergence of resistance to these drugs.
CI  - Copyright (c) 2019. Published by Elsevier Ltd.
FAU - Sharifzadeh Peyvasti, V
AU  - Sharifzadeh Peyvasti V
AD  - Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares 
      University, Tehran, Iran.
FAU - Mohabati Mobarez, A
AU  - Mohabati Mobarez A
AD  - Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares 
      University, Tehran, Iran. Electronic address: mmmobarez@modares.ac.ir.
FAU - Shahcheraghi, F
AU  - Shahcheraghi F
AD  - Department of Bacteriology, Pasteur Institute of Iran, Tehran, Iran.
FAU - Khoramabadi, N
AU  - Khoramabadi N
AD  - Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares 
      University, Tehran, Iran.
FAU - Razaz Rahmati, N
AU  - Razaz Rahmati N
AD  - Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares 
      University, Tehran, Iran.
FAU - Hosseini Doust, R
AU  - Hosseini Doust R
AD  - Department of Microbiology, Faculty of Advanced Sciences, Tehran Medical 
      Sciences, Islamic Azad University, Tehran, Iran.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190920
PL  - Netherlands
TA  - J Glob Antimicrob Resist
JT  - Journal of global antimicrobial resistance
JID - 101622459
RN  - 0 (Aminoglycosides)
RN  - 0 (Bacterial Proteins)
RN  - 0 (Gentamicins)
RN  - Y45QSO73OB (Streptomycin)
SB  - IM
MH  - Aminoglycosides/*pharmacology
MH  - Bacterial Proteins/*genetics
MH  - Disk Diffusion Antimicrobial Tests
MH  - *Drug Resistance, Multiple, Bacterial
MH  - Enterococcus/*classification/drug effects/isolation & purification
MH  - Gentamicins/pharmacology
MH  - Humans
MH  - Iran/epidemiology
MH  - Phylogeny
MH  - Prevalence
MH  - Streptomycin/pharmacology
MH  - Urinary Tract Infections/drug therapy/*microbiology
MH  - Vancomycin Resistance
OTO - NOTNLM
OT  - Aminoglycoside resistance gene
OT  - Enterococcus
OT  - High-level aminoglycoside resistance
EDAT- 2019/09/23 06:00
MHDA- 2021/02/10 06:00
CRDT- 2019/09/23 06:00
PHST- 2018/09/28 00:00 [received]
PHST- 2019/07/04 00:00 [revised]
PHST- 2019/08/10 00:00 [accepted]
PHST- 2019/09/23 06:00 [pubmed]
PHST- 2021/02/10 06:00 [medline]
PHST- 2019/09/23 06:00 [entrez]
AID - S2213-7165(19)30206-1 [pii]
AID - 10.1016/j.jgar.2019.08.008 [doi]
PST - ppublish
SO  - J Glob Antimicrob Resist. 2020 Mar;20:318-323. doi: 10.1016/j.jgar.2019.08.008. 
      Epub 2019 Sep 20.