PMID- 31545417
OWN - NLM
STAT- MEDLINE
DCOM- 20200727
LR  - 20200727
IS  - 1791-244X (Electronic)
IS  - 1107-3756 (Print)
IS  - 1107-3756 (Linking)
VI  - 44
IP  - 5
DP  - 2019 Nov
TI  - Identification of immunodominant IgE epitopes of the major house dust mite 
      allergen Der f 24.
PG  - 1888-1898
LID - 10.3892/ijmm.2019.4345 [doi]
AB  - Previously, a ubiquinol‑cytochrome c reductase binding protein (UQCRB) homolog 
      was identified in the house dust mite (HDM) species Dermatophagoides farinae (Der 
      f) as a major allergen. In the present study, the immunodominant immunoglobulin E 
      (IgE) epitope of the protein Der f 24 was investigated. Analysis of the 
      homologous amino acid (aa) sequences in Der f and human UQCRB was performed. Four 
      different recombinant Der f 24 and hybrid proteins formed by integrating Der f 
      and human UQCRB sequences were expressed in Escherichia coli, purified using 
      Ni‑NTA resins, and IgE‑binding activity was determined using IgE‑western blotting 
      and enzyme‑linked immunosorbent assay (ELISA) experiments. IgE epitopes were 
      further identified by IgE‑dot blotting and IgE‑ELISA with synthetic polypeptides 
      and HDM‑allergic sera. Three‑dimensional (3D) structural modeling was used to 
      analyze the position of the immunodominant IgE epitope. The amino acid sequence 
      homology between Der f 24 and the human UQCRB protein was determined to be 
      39.34%. IgE‑ELISA and western blot analysis showed that all of the Der f‑human 
      UQCRB hybrid proteins generated, except for the one lacking 59 residues of the 
      N‑terminal region of Der f 24, were bound by allergic serum IgE. A synthetic 
      polypeptide consisting of 32 residues of the N‑terminal reacted with IgEs from 
      HDM‑allergic sera and could be used to generate high titer specific IgG or 
      specific IgE antibodies in immunized mice. The 32‑aa N‑terminal region of Der f 
      24 was localized to a structural protrusion, which may facilitate specific 
      IgE‑binding. These results indicate that the immunodominant IgE epitope of Der f 
      24 is located mainly in a 32‑residue region of the N‑terminus. These findings may 
      inform the mechanisms of HDM allergy sensitization and allergy immunotherapy 
      development.
FAU - Cai, Ze-Lang
AU  - Cai ZL
AD  - Department of Biochemistry and Molecular Biology, Health Science Center of 
      Shenzhen University, Shenzhen, Guangdong 518060, P.R. China.
FAU - Zhang, Zhen
AU  - Zhang Z
AD  - Department of Biochemistry and Molecular Biology, Health Science Center of 
      Shenzhen University, Shenzhen, Guangdong 518060, P.R. China.
FAU - Luo, Wen-Li
AU  - Luo WL
AD  - Department of Biochemistry and Molecular Biology, Health Science Center of 
      Shenzhen University, Shenzhen, Guangdong 518060, P.R. China.
FAU - Hou, Yi-Bo
AU  - Hou YB
AD  - Department of Biochemistry and Molecular Biology, Health Science Center of 
      Shenzhen University, Shenzhen, Guangdong 518060, P.R. China.
FAU - He, Yong-Shen
AU  - He YS
AD  - Department of Biochemistry and Molecular Biology, Health Science Center of 
      Shenzhen University, Shenzhen, Guangdong 518060, P.R. China.
FAU - Chen, Jia-Jie
AU  - Chen JJ
AD  - Department of Biochemistry and Molecular Biology, Health Science Center of 
      Shenzhen University, Shenzhen, Guangdong 518060, P.R. China.
FAU - Ji, Kunmei
AU  - Ji K
AD  - Department of Biochemistry and Molecular Biology, Health Science Center of 
      Shenzhen University, Shenzhen, Guangdong 518060, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20190918
PL  - Greece
TA  - Int J Mol Med
JT  - International journal of molecular medicine
JID - 9810955
RN  - 0 (Allergens)
RN  - 0 (Antigens, Dermatophagoides)
RN  - 0 (Arthropod Proteins)
RN  - 0 (Immunodominant Epitopes)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Allergens/*immunology
MH  - Animals
MH  - Antigens, Dermatophagoides/*immunology
MH  - Arthropod Proteins/immunology
MH  - Cell Line, Tumor
MH  - Dermatophagoides farinae/*immunology
MH  - Female
MH  - Humans
MH  - Hypersensitivity/immunology
MH  - Immunodominant Epitopes/*immunology
MH  - Immunoglobulin E/*immunology
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Middle Aged
MH  - Pyroglyphidae/*immunology
MH  - Rats
MH  - Young Adult
PMC - PMC6777665
EDAT- 2019/09/24 06:00
MHDA- 2020/07/28 06:00
PMCR- 2019/09/18
CRDT- 2019/09/24 06:00
PHST- 2019/06/05 00:00 [received]
PHST- 2019/08/16 00:00 [accepted]
PHST- 2019/09/24 06:00 [pubmed]
PHST- 2020/07/28 06:00 [medline]
PHST- 2019/09/24 06:00 [entrez]
PHST- 2019/09/18 00:00 [pmc-release]
AID - ijmm-44-05-1888 [pii]
AID - 10.3892/ijmm.2019.4345 [doi]
PST - ppublish
SO  - Int J Mol Med. 2019 Nov;44(5):1888-1898. doi: 10.3892/ijmm.2019.4345. Epub 2019 
      Sep 18.