PMID- 31545493
OWN - NLM
STAT- MEDLINE
DCOM- 20200228
LR  - 20200228
IS  - 1791-3004 (Electronic)
IS  - 1791-2997 (Print)
IS  - 1791-2997 (Linking)
VI  - 20
IP  - 5
DP  - 2019 Nov
TI  - Role of CCR7 on dendritic cell‑mediated immune tolerance in the airways of 
      allergy‑induced asthmatic rats.
PG  - 4425-4432
LID - 10.3892/mmr.2019.10694 [doi]
AB  - Dendritic cells (DCs) have an important role in initiating and maintaining the 
      immune inflammatory response in allergic asthma, and CC chemokine 
      receptor 7 (CCR7) is directly involved in the pathogenesis of DC‑ and 
      T cell‑mediated allergic asthma. The present study aimed to investigate the 
      effects of CCR7 on DC‑mediated immune tolerance in allergic asthma. In the 
      present study, bone marrow‑derived DCs were transfected with an adenovirus 
      encoding the rat CCR7 gene or a short hairpin RNA targeting CCR7 (sh‑CCR7). Rats 
      injected with DCs overexpressing CCR7 or presenting CCR7 knockdown were examined. 
      After the rats were injected with DCs via the tail vein, bronchoalveolar lavage 
      fluid was collected to assess its cellular composition. The protein expression 
      levels of CCR7 in DCs were determined using immunohistochemistry and western blot 
      analysis. The protein expression levels of interferon‑gamma (IFN‑gamma), interleukin‑4 
      (IL‑4), IL‑10, IL‑12, transforming growth factor‑beta (TGF‑beta) and immunoglobulin E 
      (IgE) were determined by ELISA. Compared with the control group, the protein 
      expression level of CCR7 was significantly higher in the CCR7 overexpression 
      group and significantly lower in sh‑CCR7 group. Similarly, the number of DCs was 
      higher in the CCR7 overexpression group and lower in the sh‑CCR7 group. The 
      protein expression levels of IL‑10 and TGF‑beta were significantly lower in the CCR7 
      overexpression group and higher in the sh‑CCR7 group. In addition, the expression 
      levels of IL‑4, IL‑12, IFN‑gamma and IgE were higher in the CCR7 overexpression group 
      and lower in the sh‑CCR7 group. The present results suggested that the role of 
      cytokines and IgE in immune inflammation and immune tolerance in allergic asthma 
      may be associated with the expression level of CCR7 in DCs, suggesting that CCR7 
      may serve a role in DC‑mediated immune tolerance in allergic asthma.
FAU - Li, Yi
AU  - Li Y
AD  - Department of Respiration Medicine, People's Hospital of Shanxi Province, 
      Taiyuan, Shanxi 030001, P.R. China.
FAU - Du, Yongcheng
AU  - Du Y
AD  - Department of Respiration Medicine, People's Hospital of Shanxi Province, 
      Taiyuan, Shanxi 030001, P.R. China.
FAU - Zhang, Aizhen
AU  - Zhang A
AD  - Department of Respiration Medicine, People's Hospital of Shanxi Province, 
      Taiyuan, Shanxi 030001, P.R. China.
FAU - Jiang, Rui
AU  - Jiang R
AD  - Department of Respiration Medicine, People's Hospital of Shanxi Province, 
      Taiyuan, Shanxi 030001, P.R. China.
FAU - Nie, Xin
AU  - Nie X
AD  - Department of Respiration Medicine, People's Hospital of Shanxi Province, 
      Taiyuan, Shanxi 030001, P.R. China.
FAU - Xiong, Xue
AU  - Xiong X
AD  - Department of Respiration Medicine, People's Hospital of Shanxi Province, 
      Taiyuan, Shanxi 030001, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20190920
PL  - Greece
TA  - Mol Med Rep
JT  - Molecular medicine reports
JID - 101475259
RN  - 0 (Biomarkers)
RN  - 0 (Cytokines)
RN  - 0 (Inflammation Mediators)
RN  - 0 (Receptors, CCR7)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Animals
MH  - Asthma/*etiology/*metabolism/pathology
MH  - Biomarkers
MH  - Cytokines/metabolism
MH  - Dendritic Cells/*immunology/*metabolism
MH  - Disease Susceptibility
MH  - Gene Expression Profiling
MH  - *Immune Tolerance
MH  - Immunoglobulin E/immunology
MH  - Immunohistochemistry
MH  - Inflammation Mediators/metabolism
MH  - Rats
MH  - Receptors, CCR7/genetics/*metabolism
PMC - PMC6797982
EDAT- 2019/09/24 06:00
MHDA- 2020/02/29 06:00
PMCR- 2019/09/20
CRDT- 2019/09/24 06:00
PHST- 2018/04/10 00:00 [received]
PHST- 2019/04/26 00:00 [accepted]
PHST- 2019/09/24 06:00 [pubmed]
PHST- 2020/02/29 06:00 [medline]
PHST- 2019/09/24 06:00 [entrez]
PHST- 2019/09/20 00:00 [pmc-release]
AID - mmr-20-05-4425 [pii]
AID - 10.3892/mmr.2019.10694 [doi]
PST - ppublish
SO  - Mol Med Rep. 2019 Nov;20(5):4425-4432. doi: 10.3892/mmr.2019.10694. Epub 2019 Sep 
      20.