PMID- 31546864
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200930
IS  - 2075-4450 (Print)
IS  - 2075-4450 (Electronic)
IS  - 2075-4450 (Linking)
VI  - 10
IP  - 9
DP  - 2019 Sep 19
TI  - Isolation of a Pericentromeric Satellite DNA Family in Chnootriba argus 
      (Henosepilachna argus) with an Unusual Short Repeat Unit (TTAAAA) for Beetles.
LID - 10.3390/insects10090306 [doi]
LID - 306
AB  - Ladybird beetles (Coccinellidae) are one of the largest groups of beetles. Among 
      them, some species are of economic interest since they can act as a biological 
      control for some agricultural pests whereas other species are phytophagous and 
      can damage crops. Chnootriba argus (Coccinellidae, Epilachnini) has large 
      heterochromatic pericentromeric blocks on all chromosomes, including both sexual 
      chromosomes. Classical digestion of total genomic DNA using restriction 
      endonucleases failed to find the satellite DNA located on these heterochromatic 
      regions. Cloning of C0t-1 DNA resulted in the isolation of a repetitive DNA with 
      a repeat unit of six base pairs, TTAAAA. The amount of TTAAAA repeat in the C. 
      argus genome was about 20%. Fluorescence in situ hybridization (FISH) analysis 
      and digestion of chromosomes with the endonuclease Tru9I revealed that this 
      repetitive DNA could be considered as the putative pericentromeric satellite DNA 
      (satDNA) in this species. The presence of this satellite DNA was tested in other 
      species of the tribe Epilachnini and it is also present in Epilachna paenulata. 
      In both species, the TTAAAA repeat seems to be the main satellite DNA and it is 
      located on the pericentromeric region on all chromosomes. The size of this 
      satDNA, which has only six base pairs is unusual in Coleoptera satellite DNAs, 
      where satDNAs usually have repeat units of a much larger size. Southern 
      hybridization and FISH proved that this satDNA is conserved in some Epilachnini 
      species but not in others. This result is in concordance with the controversial 
      phylogenetic relationships among the genera of the tribe Epilachnini, where the 
      limits between genera are unclear.
FAU - Mora, Pablo
AU  - Mora P
AD  - Department of Experimental Biology, Genetic Area, University of Jaen, 23071 Jaen, 
      Spain. pmora@ujaen.es.
FAU - Vela, Jesus
AU  - Vela J
AD  - Department of Experimental Biology, Genetic Area, University of Jaen, 23071 Jaen, 
      Spain. jvela@ujaen.es.
FAU - Ruiz-Mena, Areli
AU  - Ruiz-Mena A
AD  - Department of Experimental Biology, Genetic Area, University of Jaen, 23071 Jaen, 
      Spain. armena@ujaen.es.
FAU - Palomeque, Teresa
AU  - Palomeque T
AUID- ORCID: 0000-0002-7144-4931
AD  - Department of Experimental Biology, Genetic Area, University of Jaen, 23071 Jaen, 
      Spain. tpalome@ujaen.es.
FAU - Lorite, Pedro
AU  - Lorite P
AUID- ORCID: 0000-0002-9692-5870
AD  - Department of Experimental Biology, Genetic Area, University of Jaen, 23071 Jaen, 
      Spain. plorite@ujaen.es.
LA  - eng
GR  - Program "Plan de Apoyo a la Investigacion 2019-2020", Accion 1/Universidad de 
      Jaen/
PT  - Journal Article
DEP - 20190919
PL  - Switzerland
TA  - Insects
JT  - Insects
JID - 101574235
PMC - PMC6780895
OTO - NOTNLM
OT  - C0t-1 DNA
OT  - Coccinellidae
OT  - Coleoptera
OT  - bryony ladybird
OT  - heterochromatin
OT  - in situ hybridization
OT  - satellite DNA
COIS- The authors declare no conflict of interest.
EDAT- 2019/09/25 06:00
MHDA- 2019/09/25 06:01
PMCR- 2019/09/19
CRDT- 2019/09/25 06:00
PHST- 2019/07/24 00:00 [received]
PHST- 2019/09/11 00:00 [revised]
PHST- 2019/09/17 00:00 [accepted]
PHST- 2019/09/25 06:00 [entrez]
PHST- 2019/09/25 06:00 [pubmed]
PHST- 2019/09/25 06:01 [medline]
PHST- 2019/09/19 00:00 [pmc-release]
AID - insects10090306 [pii]
AID - insects-10-00306 [pii]
AID - 10.3390/insects10090306 [doi]
PST - epublish
SO  - Insects. 2019 Sep 19;10(9):306. doi: 10.3390/insects10090306.