PMID- 31548061
OWN - NLM
STAT- MEDLINE
DCOM- 20200217
LR  - 20200217
IS  - 1525-3198 (Electronic)
IS  - 0022-0302 (Linking)
VI  - 102
IP  - 12
DP  - 2019 Dec
TI  - Antimicrobial resistance and genetic characterization of coagulase-negative 
      staphylococci from bovine mastitis milk samples in Korea.
PG  - 11439-11448
LID - S0022-0302(19)30837-9 [pii]
LID - 10.3168/jds.2019-17028 [doi]
AB  - Coagulase-negative staphylococci (CNS) are one of the most common bovine mastitis 
      pathogens found worldwide. In this study, we investigated the prevalence and 
      distribution of CNS species in mastitis milk samples and further characterized 
      the methicillin-resistant (MR) CNS. A total of 311 CNS were isolated from 3,692 
      quarter milk samples from 1,373 dairy cattle at 81 farms between 2013 and 2017. 
      Further evaluation of the CNS isolates revealed 14 CNS species among the samples 
      and 3 predominant species-namely, Staphylococcus chromogenes, Staphylococcus 
      simulans, and Staphylococcus epidermidis. Resistance was higher in S. epidermidis 
      than in other CNS species except for resistance against oxacillin in 
      Staphylococcus sciuri. Resistance to beta-lactams was the most common in all CNS 
      species (8.4% in ampicillin, 21.2% in oxacillin, and 13.5% in penicillin). 
      Conversely, only minimal resistance to cephalothin, ceftiofur, and 
      pirlimycin/novobiocin was found. Twenty-one isolates from 4 species were 
      mecA-carrying MRCNS strains, including 18 S. epidermidis and 1 each of S. sciuri, 
      Staphylococcus equorum, and Staphylococcus hominis. The majority of the 
      mecA-carrying MRCNS isolates were produced in the biofilm. Furthermore, 
      multidrug-resistant sequence type 179 isolate produced the strongest biofilm. 
      Seven genotypes were detected in the 18 MR S. epidermidis strains, the most 
      predominant of which persisted on a farm for 2 yr. Our findings for the 
      antimicrobial susceptibility profiles and genotypic characterization of the MRCNS 
      isolates could provide valuable information for controlling the spread of 
      resistance and the selection of appropriate antimicrobial therapies for mastitis 
      in the future. Further, strategic antibiotic use for mastitis treatment and 
      hygienic management practices aimed at the prevention of the growth of resistant 
      bacteria are urgently needed on dairy farms.
CI  - Copyright (c) 2019 American Dairy Science Association. Published by Elsevier Inc. 
      All rights reserved.
FAU - Kim, Su-Jeong
AU  - Kim SJ
AD  - Bacterial Disease Division, Animal and Plant Quarantine Agency, 177 Hyeksin 8-ro, 
      Gimcheon-si, Gyeongsangbuk-do 39660, Republic of Korea.
FAU - Moon, Dong Chan
AU  - Moon DC
AD  - Bacterial Disease Division, Animal and Plant Quarantine Agency, 177 Hyeksin 8-ro, 
      Gimcheon-si, Gyeongsangbuk-do 39660, Republic of Korea.
FAU - Park, Seung-Chun
AU  - Park SC
AD  - Laboratory of Veterinary Pharmacokinetics and Pharmacodynamics, College of 
      Veterinary Medicine, Kyungpook National University, Bukgu, Daegu 41566, Republic 
      of Korea.
FAU - Kang, Hee Young
AU  - Kang HY
AD  - Bacterial Disease Division, Animal and Plant Quarantine Agency, 177 Hyeksin 8-ro, 
      Gimcheon-si, Gyeongsangbuk-do 39660, Republic of Korea.
FAU - Na, Seok Hyeon
AU  - Na SH
AD  - Bacterial Disease Division, Animal and Plant Quarantine Agency, 177 Hyeksin 8-ro, 
      Gimcheon-si, Gyeongsangbuk-do 39660, Republic of Korea.
FAU - Lim, Suk-Kyung
AU  - Lim SK
AD  - Bacterial Disease Division, Animal and Plant Quarantine Agency, 177 Hyeksin 8-ro, 
      Gimcheon-si, Gyeongsangbuk-do 39660, Republic of Korea. Electronic address: 
      imsk0049@korea.kr.
LA  - eng
PT  - Journal Article
DEP - 20190920
PL  - United States
TA  - J Dairy Sci
JT  - Journal of dairy science
JID - 2985126R
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Coagulase)
RN  - UH95VD7V76 (Oxacillin)
SB  - IM
MH  - Animals
MH  - Anti-Bacterial Agents/*pharmacology
MH  - Cattle
MH  - Coagulase/*genetics
MH  - Drug Resistance, Bacterial
MH  - Farms
MH  - Female
MH  - Genotype
MH  - Mastitis, Bovine/epidemiology/*microbiology
MH  - Milk/*microbiology
MH  - Oxacillin/pharmacology
MH  - Prevalence
MH  - Republic of Korea/epidemiology
MH  - Staphylococcal Infections/epidemiology/microbiology/*veterinary
MH  - Staphylococcus/*drug effects
MH  - Staphylococcus epidermidis/drug effects
OTO - NOTNLM
OT  - antimicrobial resistance
OT  - coagulase-negative staphylococci
OT  - mastitis in dairy cattle
OT  - methicillin resistance
OT  - molecular typing
EDAT- 2019/09/25 06:00
MHDA- 2020/02/18 06:00
CRDT- 2019/09/25 06:00
PHST- 2019/05/28 00:00 [received]
PHST- 2019/08/05 00:00 [accepted]
PHST- 2019/09/25 06:00 [pubmed]
PHST- 2020/02/18 06:00 [medline]
PHST- 2019/09/25 06:00 [entrez]
AID - S0022-0302(19)30837-9 [pii]
AID - 10.3168/jds.2019-17028 [doi]
PST - ppublish
SO  - J Dairy Sci. 2019 Dec;102(12):11439-11448. doi: 10.3168/jds.2019-17028. Epub 2019 
      Sep 20.