PMID- 31548367
OWN - NLM
STAT- MEDLINE
DCOM- 20210618
LR  - 20210618
IS  - 2373-2822 (Electronic)
IS  - 2373-2822 (Linking)
VI  - 7
IP  - 2
DP  - 2020 Mar/Apr
TI  - Chronic Ethanol Differentially Modulates Glutamate Release from Dorsal and 
      Ventral Prefrontal Cortical Inputs onto Rat Basolateral Amygdala Principal 
      Neurons.
LID - ENEURO.0132-19.2019 [pii]
LID - 10.1523/ENEURO.0132-19.2019 [doi]
AB  - The medial prefrontal cortex (mPFC) and the basolateral amygdala (BLA) have 
      strong reciprocal connectivity. Projections from the BLA to the mPFC can drive 
      innate, anxiety-related behaviors, but it is unclear whether reciprocal 
      projections from the mPFC to BLA have similar roles. Here, we use optogenetics 
      and chemogenetics to characterize the neurophysiological and behavioral 
      alterations produced by chronic ethanol exposure and withdrawal on dorsal mPFC 
      (dmPFC) and ventral mPFC (vmPFC) medial prefrontal cortical terminals in the BLA. 
      We exposed adult male Sprague Dawley rats to chronic intermittent ethanol (CIE) 
      using vapor chambers, measured anxiety-like behavior on the elevated zero maze, 
      and used electrophysiology to record glutamatergic and GABAergic responses in BLA 
      principal neurons. We found that withdrawal from a 7 d CIE exposure produced 
      opposing effects at dmPFC (increased glutamate release) and vmPFC (decreased 
      glutamate release) terminals in the BLA. Chemogenetic inhibition of dmPFC 
      terminals in the BLA attenuated the increased anxiety-like behavior we observed 
      during withdrawal. These data demonstrate that chronic ethanol exposure and 
      withdrawal strengthen the synaptic connections between the dmPFC and BLA but 
      weakens the vmPFC-BLA pathway. Moreover, facilitation of the dmPFC-BLA pathway 
      during withdrawal contributes to anxiety-like behavior. Given the opposing roles 
      of dmPFC-BLA and vmPFC-BLA pathways in fear conditioning, our results suggest 
      that chronic ethanol exposure simultaneously facilitates circuits involved in the 
      acquisition of and diminishes circuits involved with the extinction of 
      withdrawal-related aversive behaviors.
CI  - Copyright (c) 2020 McGinnis et al.
FAU - McGinnis, Molly M
AU  - McGinnis MM
AD  - Department of Physiology and Pharmacology, Wake Forest School of Medicine, 
      Winston-Salem, North Carolina 27157.
FAU - Parrish, Brian C
AU  - Parrish BC
AD  - Department of Physiology and Pharmacology, Wake Forest School of Medicine, 
      Winston-Salem, North Carolina 27157.
FAU - Chappell, Ann M
AU  - Chappell AM
AUID- ORCID: 0000-0002-0614-2574
AD  - Department of Physiology and Pharmacology, Wake Forest School of Medicine, 
      Winston-Salem, North Carolina 27157.
FAU - Alexander, Nancy J
AU  - Alexander NJ
AUID- ORCID: 0000-0003-4451-4874
AD  - Department of Physiology and Pharmacology, Wake Forest School of Medicine, 
      Winston-Salem, North Carolina 27157.
FAU - McCool, Brian A
AU  - McCool BA
AUID- ORCID: 0000-0002-1002-9435
AD  - Department of Physiology and Pharmacology, Wake Forest School of Medicine, 
      Winston-Salem, North Carolina 27157 Bmccool@wakehealth.edu.
LA  - eng
GR  - P50 AA026117/AA/NIAAA NIH HHS/United States
GR  - R21 AA026572/AA/NIAAA NIH HHS/United States
GR  - R01 AA023999/AA/NIAAA NIH HHS/United States
GR  - T32 AA007565/AA/NIAAA NIH HHS/United States
GR  - F31 AA025514/AA/NIAAA NIH HHS/United States
GR  - R01 AA014445/AA/NIAAA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20200306
PL  - United States
TA  - eNeuro
JT  - eNeuro
JID - 101647362
RN  - 3K9958V90M (Ethanol)
RN  - 3KX376GY7L (Glutamic Acid)
SB  - IM
MH  - Amygdala
MH  - Animals
MH  - *Basolateral Nuclear Complex
MH  - Ethanol
MH  - Glutamic Acid
MH  - Male
MH  - Neurons
MH  - Prefrontal Cortex
MH  - Rats
MH  - Rats, Sprague-Dawley
PMC - PMC7070451
OTO - NOTNLM
OT  - infralimbic
OT  - patch-clamp electrophysiology
OT  - prelimbic
OT  - presynaptic
EDAT- 2019/09/25 06:00
MHDA- 2021/06/22 06:00
PMCR- 2020/03/04
CRDT- 2019/09/25 06:00
PHST- 2019/04/05 00:00 [received]
PHST- 2019/05/12 00:00 [revised]
PHST- 2019/08/23 00:00 [accepted]
PHST- 2019/09/25 06:00 [pubmed]
PHST- 2021/06/22 06:00 [medline]
PHST- 2019/09/25 06:00 [entrez]
PHST- 2020/03/04 00:00 [pmc-release]
AID - ENEURO.0132-19.2019 [pii]
AID - eN-NWR-0132-19 [pii]
AID - 10.1523/ENEURO.0132-19.2019 [doi]
PST - epublish
SO  - eNeuro. 2020 Mar 6;7(2):ENEURO.0132-19.2019. doi: 10.1523/ENEURO.0132-19.2019. 
      Print 2020 Mar/Apr.