PMID- 31549885
OWN - NLM
STAT- MEDLINE
DCOM- 20200604
LR  - 20200604
IS  - 1532-4311 (Electronic)
IS  - 0882-0139 (Linking)
VI  - 49
IP  - 1-2
DP  - 2020 Feb
TI  - Association of HLA-G 3'UTR Polymorphisms with Soluble HLA-G Levels and Disease 
      Activity in Patients with Rheumatoid Arthritis: A Case-Control Study.
PG  - 88-105
LID - 10.1080/08820139.2019.1657146 [doi]
AB  - Background: Human leukocyte antigen (HLA)-G antigens are inducible non-classical 
      major histocompatibility complex class Ib molecules which play an important role 
      in the regulation of inflammatory processes and immunomodulation in autoimmune 
      diseases. There are controversial reports on the impact of HLA-G gene 
      polymorphisms on rheumatoid arthritis (RA). This study aimed at examining the 
      impact of 14 base pair (bp) ins/del (rs66554220) and +3142G>C (rs1063320) 
      polymorphisms and correlating these with soluble HLA-G (sHLA-G) levels to 
      understand the susceptibility to RA in our sample cohort.Methods: Genomic DNA 
      from 140 RA patients and 125 healthy controls was isolated using the salting out 
      method. The genotyping of two polymorphisms of HLA-G (+3142G>C and 14 bp ins/del) 
      was done by polymerase chain reaction restriction fragment length polymorphism 
      (PCR-RFLP) and PCR method, respectively. Levels of sHLA-G were estimated by ELISA 
      and disease activity was calculated by disease activity score 
      (DAS28-ESR).Results: The HLA-G +3142G>C polymorphism was found to be associated 
      with a decreased risk of RA as attributed to recessive inheritance tested model 
      results (OR = 0.4, 95%C.I. = 0.2-0.9, p = .0313*, GG + GC versus CC). Our finding 
      did not support an association between HLA-G 14 bp ins/del variant and 
      risk/protection of RA. The sHLA-G levels were significantly lower in +3142GG and 
      +3142GC RA patients as compared to healthy controls.Conclusion: HLA-G +3142G>C 
      gene polymorphism might decrease the risk of occurrence of RA in our sample 
      cohort as +3142CC genotype is associated with increased sHLA-G 
      levels.Abbreviations: HLA-G: human leukocyte antigen-G; RA: rheumatoid arthritis; 
      MHC: major histocompatibility complex; UTR: untranslated region; URR: upstream 
      regulatory region; SLE: systemic lupus erythematous; PCR-RFLP: polymerase chain 
      reaction restriction fragment length polymorphism; sHLA-G: soluble HLA-G; bp: 
      base pair; ACR/EULAR: American College of Rheumatology/European League against 
      Rheumatism; RF: rheumatoid factor; Anti-CCP: anti-cyclic citrullinated peptide; 
      DAS28-ESR: Disease Activity Score 28- Erythrocyte Sedimentation Rate; TJC: tender 
      joint count; SJC: swollen joint count; ESR: erythrocyte sedimentation rate; PGA: 
      patient global assessment; HTN: hypertension; DM: diabetes mellitus; TB: 
      tuberculosis; IEC: Institute Ethics Committee; ELISA: enzyme linked immunosorbent 
      assay; ROC: receiver operating characteristics; AUC: area under curve; SNP: 
      single nucleotide polymorphism; MTX: methotrexate; DMARDs: disease modifying 
      anti-rheumatic drugs; Treg: regulatory T cells; IL: interleukinUnits: soluble 
      HLA-G: Units/mL U/mL.
FAU - Gautam, Surabhi
AU  - Gautam S
AD  - Laboratory for Molecular Reproduction and Genetics, Department of Anatomy, All 
      India Institute of Medical Sciences, New Delhi, India.
FAU - Kumar, Uma
AU  - Kumar U
AD  - Department of Rheumatology, All India Institute of Medical Sciences, New Delhi, 
      India.
FAU - Kumar, Manoj
AU  - Kumar M
AD  - Laboratory for Molecular Reproduction and Genetics, Department of Anatomy, All 
      India Institute of Medical Sciences, New Delhi, India.
FAU - Kanga, Uma
AU  - Kanga U
AD  - Department of Transplant Immunology and Immunogenetics, All India Institute of 
      Medical Sciences, New Delhi, India.
FAU - Dada, Rima
AU  - Dada R
AD  - Laboratory for Molecular Reproduction and Genetics, Department of Anatomy, All 
      India Institute of Medical Sciences, New Delhi, India.
LA  - eng
PT  - Journal Article
PT  - Observational Study
DEP - 20190905
PL  - England
TA  - Immunol Invest
JT  - Immunological investigations
JID - 8504629
RN  - 0 (3' Untranslated Regions)
RN  - 0 (HLA-G Antigens)
SB  - IM
MH  - 3' Untranslated Regions
MH  - Adult
MH  - Arthritis, Rheumatoid/blood/*genetics
MH  - Case-Control Studies
MH  - Female
MH  - Genotype
MH  - HLA-G Antigens/blood/*genetics
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Polymorphism, Genetic
OTO - NOTNLM
OT  - 3'UTR polymorphism
OT  - North Indian population
OT  - Rheumatoid Arthritis
OT  - disease activity score
OT  - soluble HLA-G
EDAT- 2019/09/25 06:00
MHDA- 2020/06/05 06:00
CRDT- 2019/09/25 06:00
PHST- 2019/09/25 06:00 [pubmed]
PHST- 2020/06/05 06:00 [medline]
PHST- 2019/09/25 06:00 [entrez]
AID - 10.1080/08820139.2019.1657146 [doi]
PST - ppublish
SO  - Immunol Invest. 2020 Feb;49(1-2):88-105. doi: 10.1080/08820139.2019.1657146. Epub 
      2019 Sep 5.