PMID- 31554827
OWN - NLM
STAT- MEDLINE
DCOM- 20201029
LR  - 20210110
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 9
IP  - 1
DP  - 2019 Sep 25
TI  - Prenatal Exposure to Environmentally-Relevant Contaminants Perturbs Male 
      Reproductive Parameters Across Multiple Generations that are Partially Protected 
      by Folic Acid Supplementation.
PG  - 13829
LID - 10.1038/s41598-019-50060-z [doi]
LID - 13829
AB  - The paternal environment is thought to influence sperm quality and future progeny 
      may also be impacted. We hypothesized that prenatal exposure to 
      environmentally-relevant contaminants impairs male reproduction, altering embryo 
      gene expression over multiple generations. Folic acid (FA) can improve sperm 
      quality and pregnancy outcomes, thus we further hypothesized that FA mitigates 
      the contaminants. Sprague-Dawley F0 female rats treated with persistent organic 
      pollutants (POPs) or corn oil and fed basal or supplemented FA diets, then used 
      to yield four generations of litters. Only F0 females received POPs and/or FA 
      treatments. In utero POPs exposure altered sperm parameters in F1, which were 
      partly rescued by FA supplementation. Paternal exposure to POPs reduced sperm 
      quality in F2 males, and the fertility of F3 males was modified by both POPs and 
      FA. Ancestral FA supplementation improved sperm parameters of F4 males, while the 
      POPs effect diminished. Intriguingly, F3 males had the poorest pregnancy outcomes 
      and generated the embryos with the most significantly differentially expressed 
      genes. Early-life exposure to POPs harms male reproduction across multiple 
      generations. FA supplementation partly mitigated the impact of POPs. The two-cell 
      embryo transcriptome is susceptible to paternal environment and could be the 
      foundation for later pregnancy outcomes.
FAU - Lessard, Maryse
AU  - Lessard M
AD  - Department of Animal Sciences, Faculty of Agricultural and Food Sciences, Centre 
      de recherche en reproduction, developpement et sante intergenerationnelle, Laval 
      University, Quebec City, Quebec, Canada.
FAU - Herst, Pauline M
AU  - Herst PM
AD  - Department of Animal Sciences, Faculty of Agricultural and Food Sciences, Centre 
      de recherche en reproduction, developpement et sante intergenerationnelle, Laval 
      University, Quebec City, Quebec, Canada.
FAU - Charest, Phanie L
AU  - Charest PL
AD  - Department of Animal Sciences, Faculty of Agricultural and Food Sciences, Centre 
      de recherche en reproduction, developpement et sante intergenerationnelle, Laval 
      University, Quebec City, Quebec, Canada.
FAU - Navarro, Pauline
AU  - Navarro P
AD  - Department of Nutrition, Faculty of Agricultural and Food Sciences, Centre de 
      recherche en reproduction, developpement et sante intergenerationnelle, Laval 
      University, Quebec City, Quebec, Canada.
FAU - Joly-Beauparlant, Charles
AU  - Joly-Beauparlant C
AD  - Computational Biology Laboratory Research Centre, Faculty of Medicine, Laval 
      University, Quebec City, Quebec, Canada.
FAU - Droit, Arnaud
AU  - Droit A
AD  - Computational Biology Laboratory Research Centre, Faculty of Medicine, Laval 
      University, Quebec City, Quebec, Canada.
FAU - Kimmins, Sarah
AU  - Kimmins S
AD  - Department of Pharmacology and Therapeutics, Faculty of Medicine, McGill 
      University, Montreal, Quebec, Canada.
AD  - Department of Animal Science, Faculty of Agricultural and Environmental Sciences, 
      McGill University, Montreal, Quebec, Canada.
FAU - Trasler, Jacquetta
AU  - Trasler J
AD  - Departments of Pediatrics, Human Genetics and Pharmacology & Therapeutics, McGill 
      University, Research Institute, McGill University Health Centre, Montreal, 
      Quebec, Canada.
FAU - Benoit-Biancamano, Marie-Odile
AU  - Benoit-Biancamano MO
AUID- ORCID: 0000-0003-0244-1179
AD  - Department of Pathology and Microbiology, Faculty of Veterinary Medicine, 
      University of Montreal, Saint-Hyacinthe, Quebec, Canada.
FAU - MacFarlane, Amanda J
AU  - MacFarlane AJ
AD  - Nutrition Research Division, Health Canada, Ottawa, Canada.
FAU - Dalvai, Mathieu
AU  - Dalvai M
AD  - Department of Animal Sciences, Faculty of Agricultural and Food Sciences, Centre 
      de recherche en reproduction, developpement et sante intergenerationnelle, Laval 
      University, Quebec City, Quebec, Canada.
FAU - Bailey, Janice L
AU  - Bailey JL
AUID- ORCID: 0000-0002-5490-6946
AD  - Department of Animal Sciences, Faculty of Agricultural and Food Sciences, Centre 
      de recherche en reproduction, developpement et sante intergenerationnelle, Laval 
      University, Quebec City, Quebec, Canada. Janice.Bailey@fsaa.ulaval.ca.
LA  - eng
GR  - TE1 138294/CIHR/Canada
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190925
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 935E97BOY8 (Folic Acid)
SB  - IM
MH  - Animals
MH  - Disease Models, Animal
MH  - Environmental Pollution/*adverse effects
MH  - Female
MH  - Folic Acid/*administration & dosage/pharmacology
MH  - Gene Expression Profiling
MH  - Gene Expression Regulation, Developmental/drug effects
MH  - Male
MH  - Paternal Exposure/adverse effects
MH  - Pregnancy
MH  - Prenatal Exposure Delayed Effects/*diet therapy
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reproduction/*drug effects
MH  - Spermatozoa/*drug effects
PMC - PMC6761122
COIS- The authors declare no competing interests.
EDAT- 2019/09/27 06:00
MHDA- 2020/10/30 06:00
PMCR- 2019/09/25
CRDT- 2019/09/27 06:00
PHST- 2019/06/07 00:00 [received]
PHST- 2019/08/23 00:00 [accepted]
PHST- 2019/09/27 06:00 [entrez]
PHST- 2019/09/27 06:00 [pubmed]
PHST- 2020/10/30 06:00 [medline]
PHST- 2019/09/25 00:00 [pmc-release]
AID - 10.1038/s41598-019-50060-z [pii]
AID - 50060 [pii]
AID - 10.1038/s41598-019-50060-z [doi]
PST - epublish
SO  - Sci Rep. 2019 Sep 25;9(1):13829. doi: 10.1038/s41598-019-50060-z.