PMID- 31555348
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1792-0981 (Print)
IS  - 1792-1015 (Electronic)
IS  - 1792-0981 (Linking)
VI  - 18
IP  - 4
DP  - 2019 Oct
TI  - LncRNA loc285194 inhibits tumor growth of laryngeal squamous cell carcinoma cells 
      by downregulating hexokinase 2.
PG  - 2378-2384
LID - 10.3892/etm.2019.7761 [doi]
AB  - LncRNA loc285194 is a well-characterized tumor suppressor in several types of 
      cancer. To the best of our knowledge, the present study is the first to 
      investigate the involvement of lncRNA loc285194 in laryngeal squamous cell 
      carcinoma (LSCC). The expression of loc285194 and hexokinase 2 (HK-2) mRNA in 
      laryngeal biopsies of patients with LSCC or healthy controls was detected by 
      reverse transcription-quantitative polymerase chain reaction (RT-qPCR). The 
      diagnostic value of loc285194 and HK-2 mRNA for LSCC was evaluated using receiver 
      operating characteristic curve analysis. The correlation between expression of 
      loc285194 and HK-2 mRNA was analyzed using the Pearson correlation coefficient. 
      The association between loc285194 and clinicopathological data of patients with 
      LSCC was analyzed using the Chi-square test. LncRNA loc285194 and HK-2 expression 
      vectors were transfected into human LSCC cell lines and the effects on HK-2 
      expression, lncRNA loc285194 expression and cell proliferation was detected by 
      western blot, RT-qPCR and CCK-8 assays, respectively. It was observed that 
      loc285194 was upregulated, while HK-2 mRNA was downregulated in patients with 
      LSCC compared with healthy controls. The results demonstrated that the 
      downregulation of loc285194 may be a sensitive diagnostic marker for LSCC. The 
      expression levels of loc285194 and HK-2 mRNA were inversely correlated in 
      patients with LSCC, but not in healthy controls. loc285194 expression was 
      significantly associated with tumor size, but not distant tumor metastasis. 
      Loc285194 overexpression significantly inhibited HK-2 expression and LSCC cell 
      proliferation. HK-2 overexpression did not significantly affect Loc285194 
      expression, but promoted LSCC proliferation and attenuated the inhibitory effects 
      of loc285194 overexpression on LSCC cell proliferation. lncRNA loc285194 may 
      inhibit tumor growth of LSCC cells by downregulating HK-2.
FAU - Gao, Yan
AU  - Gao Y
AD  - Department of Otorhinolaryngology, The Second Affiliated Hospital Xinjiang 
      Medical University, Urumqi, Xinjiang 830000, P.R. China.
FAU - Wang, Zhongqiao
AU  - Wang Z
AD  - Department of Otorhinolaryngology, The Second Affiliated Hospital Xinjiang 
      Medical University, Urumqi, Xinjiang 830000, P.R. China.
FAU - Tong, Jing
AU  - Tong J
AD  - Department of Otorhinolaryngology, The Fifth Affiliated Hospital Xinjiang Medical 
      University, Urumqi, Xinjiang 830000, P.R. China.
FAU - Zheng, Yan
AU  - Zheng Y
AD  - Department of Otorhinolaryngology, The Second Affiliated Hospital Xinjiang 
      Medical University, Urumqi, Xinjiang 830000, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20190709
PL  - Greece
TA  - Exp Ther Med
JT  - Experimental and therapeutic medicine
JID - 101531947
PMC - PMC6755266
OTO - NOTNLM
OT  - hexokinase 2
OT  - laryngeal squamous cell carcinoma
OT  - lncRNA loc285194
OT  - proliferation
EDAT- 2019/09/27 06:00
MHDA- 2019/09/27 06:01
PMCR- 2019/07/09
CRDT- 2019/09/27 06:00
PHST- 2018/08/12 00:00 [received]
PHST- 2019/03/26 00:00 [accepted]
PHST- 2019/09/27 06:00 [entrez]
PHST- 2019/09/27 06:00 [pubmed]
PHST- 2019/09/27 06:01 [medline]
PHST- 2019/07/09 00:00 [pmc-release]
AID - ETM-0-0-7761 [pii]
AID - 10.3892/etm.2019.7761 [doi]
PST - ppublish
SO  - Exp Ther Med. 2019 Oct;18(4):2378-2384. doi: 10.3892/etm.2019.7761. Epub 2019 Jul 
      9.