PMID- 31557289
OWN - NLM
STAT- MEDLINE
DCOM- 20191230
LR  - 20200409
IS  - 1536-2442 (Electronic)
IS  - 1536-2442 (Linking)
VI  - 19
IP  - 5
DP  - 2019 Sep 1
TI  - Glucose Utilization in the Regulation of Chitin Synthesis in Brown Planthopper.
LID - 10.1093/jisesa/iez081 [doi]
LID - 3
AB  - Glucose-6-phosphatase (G6Pase) and hexokinase (HK) are two key enzymes in the 
      glycolysis and gluconeogenesis pathways, which catalyze the synthesis and 
      degradation of glucose in insects, respectively. G6Pase and HK play an important 
      role in insect growth by regulating the metabolism of glucose, leading to the 
      efficient metabolism of other macromolecules. However, it is unclear whether 
      these genes could be investigated for pest control through their actions on 
      chitin metabolism. We studied the potential functions of G6Pase and HK genes in 
      the regulation of chitin metabolism pathways by RNAi technology. Interference 
      with G6Pase expression did not affect trehalose and chitin metabolism pathways in 
      brown planthopper, Nilaparvata lugens (Stal). However, knockdown of the HK gene 
      resulted in a significant decrease of expression of genes associated with the 
      trehalose metabolic pathway but had no significant effect on trehalase activity, 
      trehalose content, or glucogen content. Additionally, HK knockdown resulting in 
      downregulation of the genes involved in chitin metabolism in the brown 
      planthopper. These insects also showed wing deformities and difficulty in molting 
      to varying degrees. We suggest that the silencing of HK expression directly 
      inhibited the decomposition of glucose, leading to impaired chitin synthesis.
CI  - (c) The Author(s) 2019. Published by Oxford University Press on behalf of 
      Entomological Society of America. All rights reserved. For permissions, please 
      e-mail: journals.permissions@oup.com.
FAU - Pan, Bi-Ying
AU  - Pan BY
AD  - Guizhou Provincial Key Laboratory for Rare Animal and Economic Insect of the 
      Mountainous Region, Department of Biology and Engineering of Environment, Guiyang 
      University, Guiyang, P.R. China.
AD  - College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, 
      Zhejiang, P.R. China.
FAU - Li, Guo-Yong
AU  - Li GY
AD  - Guizhou Provincial Key Laboratory for Rare Animal and Economic Insect of the 
      Mountainous Region, Department of Biology and Engineering of Environment, Guiyang 
      University, Guiyang, P.R. China.
FAU - Wu, Yan
AU  - Wu Y
AD  - Guizhou Provincial Key Laboratory for Rare Animal and Economic Insect of the 
      Mountainous Region, Department of Biology and Engineering of Environment, Guiyang 
      University, Guiyang, P.R. China.
FAU - Zhou, Zhong-Shi
AU  - Zhou ZS
AD  - Guizhou Provincial Key Laboratory for Rare Animal and Economic Insect of the 
      Mountainous Region, Department of Biology and Engineering of Environment, Guiyang 
      University, Guiyang, P.R. China.
FAU - Zhou, Min
AU  - Zhou M
AD  - College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, 
      Zhejiang, P.R. China.
FAU - Li, Can
AU  - Li C
AD  - Guizhou Provincial Key Laboratory for Rare Animal and Economic Insect of the 
      Mountainous Region, Department of Biology and Engineering of Environment, Guiyang 
      University, Guiyang, P.R. China.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Insect Sci
JT  - Journal of insect science (Online)
JID - 101096396
RN  - 0 (Insect Proteins)
RN  - 1398-61-4 (Chitin)
RN  - B8WCK70T7I (Trehalose)
RN  - EC 2.7.1.1 (Hexokinase)
RN  - EC 3.1.3.9 (Glucose-6-Phosphatase)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Animals
MH  - Chitin/*biosynthesis
MH  - Glucose/*metabolism
MH  - Glucose-6-Phosphatase/genetics
MH  - Hemiptera/enzymology/genetics/*metabolism
MH  - Hexokinase/genetics
MH  - Insect Proteins/genetics
MH  - RNA Interference
MH  - Trehalose/metabolism
PMC - PMC6761884
OTO - NOTNLM
OT  - brown planthopper
OT  - chitin metabolism
OT  - glucose-6-phosphatase
OT  - hexokinase
OT  - trehalose metabolism
EDAT- 2019/09/27 06:00
MHDA- 2019/12/31 06:00
PMCR- 2019/09/26
CRDT- 2019/09/27 06:00
PHST- 2019/06/18 00:00 [received]
PHST- 2019/09/27 06:00 [entrez]
PHST- 2019/09/27 06:00 [pubmed]
PHST- 2019/12/31 06:00 [medline]
PHST- 2019/09/26 00:00 [pmc-release]
AID - 5574393 [pii]
AID - iez081 [pii]
AID - 10.1093/jisesa/iez081 [doi]
PST - ppublish
SO  - J Insect Sci. 2019 Sep 1;19(5):3. doi: 10.1093/jisesa/iez081.