PMID- 3155772
OWN - NLM
STAT- MEDLINE
DCOM- 19850320
LR  - 20111117
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 134
IP  - 3
DP  - 1985 Mar
TI  - The fate of IgE bound to rat basophilic leukemia cells. IV. Functional 
      association between the receptors for IgE.
PG  - 1766-73
AB  - Rat basophilic leukemia cells (RBL-2H3) have receptors for immunoglobulin E (IgE) 
      and immunoglobulin G (IgG). These receptors for IgE mediate the endocytosis of 
      chemically or immunochemically cross-linked IgE but not monomeric IgE. However, 
      unoccupied receptors were endocytosed with cross-linked IgE. To further assess 
      the degree and specificity of the observed coendocytosis, we exposed cells 
      carrying monomeric rat IgE and monomeric mouse IgE anti-DNP to a DNP-protein 
      conjugate. We found that up to 30% of the surface-bound monomeric rat IgE 
      redistributed at 0 to 4 degrees C and was then internalized at 37 degrees C with 
      the immunochemically cross-linked mouse IgE. To assess the specificity of the 
      coendocytosis, we exposed cells carrying monomeric rat IgE to immunochemically 
      cross-linked mouse IgG. We found that the binding, patching, and endocytosis of 
      cross-linked mouse IgG had no effect on the monomerically bound rat IgE. The rate 
      of coendocytosis was the same as the rate of endocytosis (t 1/2 3 to 5 min). The 
      extent of coendocytosis depended on the extent of endocytosis but was relatively 
      insensitive to changes in the ratio between mouse and rat IgE over a broad range. 
      These results indicate that some of the receptors for IgE are associated in a 
      specific fashion.
FAU - Furuichi, K
AU  - Furuichi K
FAU - Rivera, J
AU  - Rivera J
FAU - Triche, T
AU  - Triche T
FAU - Isersky, C
AU  - Isersky C
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Cross-Linking Reagents)
RN  - 0 (Dinitrobenzenes)
RN  - 0 (Dinitrophenols)
RN  - 0 (Macromolecular Substances)
RN  - 0 (Receptors, Fc)
RN  - 0 (Receptors, IgE)
RN  - 0 (Serum Albumin)
RN  - 0 (dinitrophenyl-human serum albumin conjugate)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Animals
MH  - Basophils/*metabolism
MH  - Binding Sites, Antibody
MH  - Cross-Linking Reagents
MH  - Dinitrobenzenes/immunology
MH  - *Dinitrophenols
MH  - Endocytosis
MH  - Fluorescent Antibody Technique
MH  - Immunoglobulin E/*metabolism
MH  - Leukemia/*immunology
MH  - Macromolecular Substances
MH  - Mice
MH  - Rats
MH  - Receptors, Fc/analysis/*physiology
MH  - Receptors, IgE
MH  - Serum Albumin/metabolism
EDAT- 1985/03/01 00:00
MHDA- 1985/03/01 00:01
CRDT- 1985/03/01 00:00
PHST- 1985/03/01 00:00 [pubmed]
PHST- 1985/03/01 00:01 [medline]
PHST- 1985/03/01 00:00 [entrez]
PST - ppublish
SO  - J Immunol. 1985 Mar;134(3):1766-73.