PMID- 31558329
OWN - NLM
STAT- MEDLINE
DCOM- 20200403
LR  - 20200403
IS  - 1879-1166 (Electronic)
IS  - 0198-8859 (Linking)
VI  - 80
IP  - 11
DP  - 2019 Nov
TI  - HLA Haplotypes In 250 Families: The Baylor Laboratory Results And A Perspective 
      On A Core NGS Testing Model For The 17(th) International HLA And Immunogenetics 
      Workshop.
PG  - 897-905
LID - S0198-8859(19)31027-4 [pii]
LID - 10.1016/j.humimm.2019.07.298 [doi]
AB  - Since their inception, the International HLA & Immunogenetics Workshops (IHIW) 
      served as a collaborative platform for exchange of specimens, reference 
      materials, experiences and best practices. In this report we present a subset of 
      the results of human leukocyte antigen (HLA) haplotypes in families tested by 
      next generation sequencing (NGS) under the 17th IHIW. We characterized 961 
      haplotypes in 921 subjects belonging to 250 families from 8 countries (Argentina, 
      Austria, Egypt, Jamaica, Germany, Greece, Kuwait, and Switzerland). These samples 
      were tested in a single core laboratory in a high throughput fashion using 6 
      different reagents/software platforms. Families tested included patients 
      evaluated clinically as transplant recipients (kidney and hematopoietic cell 
      transplant) and their respective family members. We identified 486 HLA alleles at 
      the following loci HLA-A, -B, -C, -DRB1, -DRB3, -DRB4, -DRB5, -DQA1, -DQB1, 
      -DPA1, -DPB1 (77, 115, 68, 69, 10, 6, 4, 44, 31, 20 and 42 alleles, 
      respectively). We also identified nine novel alleles with polymorphisms in coding 
      regions. This approach of testing samples from multiple laboratories across the 
      world in different stages of technology implementation in a single core 
      laboratory may be useful for future international workshops. Although data 
      presented may not be reflective of allele and haplotype frequencies in the 
      countries to which the families belong, they represent an extensive collection of 
      3rd and 4th field resolution level 11-locus haplotype associations of 486 alleles 
      identified in families from 8 countries.
CI  - Copyright (c) 2019 American Society for Histocompatibility and Immunogenetics. 
      Published by Elsevier Inc. All rights reserved.
FAU - Askar, Medhat
AU  - Askar M
AD  - Baylor University Medical Center, Dallas, TX, USA; Texas A&M Health Science 
      Center College of Medicine, Bryan, TX, USA. Electronic address: 
      Medhat.Askar@BSWHealth.ORG.
FAU - Madbouly, Abeer
AU  - Madbouly A
AD  - Bioinformatics Research, Center for International Blood and Marrow Transplant 
      Research, Minneapolis, MN, USA.
FAU - Zhrebker, Leah
AU  - Zhrebker L
AD  - Baylor University Medical Center, Dallas, TX, USA.
FAU - Willis, Amanda
AU  - Willis A
AD  - Baylor University Medical Center, Dallas, TX, USA.
FAU - Kennedy, Shawna
AU  - Kennedy S
AD  - Baylor University Medical Center, Dallas, TX, USA.
FAU - Padros, Karin
AU  - Padros K
AD  - Primer Centro Argentino de Immunogenetica (PRICAI), Fundacion Favaloro, CABA, 
      Argentina.
FAU - Rodriguez, Maria Beatriz
AU  - Rodriguez MB
AD  - Primer Centro Argentino de Immunogenetica (PRICAI), Fundacion Favaloro, CABA, 
      Argentina.
FAU - Bach, Christian
AU  - Bach C
AD  - Departments of Internal Medicine & Hematology and Oncology, 
      Friedrich-Alexander-University Erlangen-Nurnberg, Germany.
FAU - Spriewald, Bernd
AU  - Spriewald B
AD  - Departments of Internal Medicine & Hematology and Oncology, 
      Friedrich-Alexander-University Erlangen-Nurnberg, Germany.
FAU - Ameen, Reem
AU  - Ameen R
AD  - Health Sciences Center, Kuwait University, Jabriya, Kuwait.
FAU - Shemmari, Salem Al
AU  - Shemmari SA
AD  - Health Sciences Center, Kuwait University, Jabriya, Kuwait.
FAU - Tarassi, Katerina
AU  - Tarassi K
AD  - Evangelismos Hospital, Athens, Greece.
FAU - Tsirogianni, Alexandra
AU  - Tsirogianni A
AD  - Evangelismos Hospital, Athens, Greece.
FAU - Hamdy, Nayera
AU  - Hamdy N
AD  - National Cancer Institute, Cairo University, Cairo, Egypt.
FAU - Mossallam, Ghada
AU  - Mossallam G
AD  - National Cancer Institute, Cairo University, Cairo, Egypt.
FAU - Honger, Gideon
AU  - Honger G
AD  - Transplantation Immunology, Department of Biomedicine, University Hospital Basel, 
      Basel, Switzerland; HLA-Diagnostics and Immunogenetics, Department of Laboratory 
      Medicine, University Hospital Basel, Basel, Switzerland.
FAU - Spinnler, Regina
AU  - Spinnler R
AD  - HLA-Diagnostics and Immunogenetics, Department of Laboratory Medicine, University 
      Hospital Basel, Basel, Switzerland.
FAU - Fischer, Gottfried
AU  - Fischer G
AD  - Medical University of Vienna, Vienna, Austria.
FAU - Fae, Ingrid
AU  - Fae I
AD  - Medical University of Vienna, Vienna, Austria.
FAU - Charlton, Ronald
AU  - Charlton R
AD  - Caribbean Bone Marrow Registry, Plantation, FL, USA; Laboratory Consultants of 
      Florida, Jacksonville, FL, USA.
FAU - Dunk, Arthur
AU  - Dunk A
AD  - Caribbean Bone Marrow Registry, Plantation, FL, USA.
FAU - Vayntrub, Tamara A
AU  - Vayntrub TA
AD  - Stanford Blood Center, Palo Alto, CA, USA.
FAU - Halagan, Michael
AU  - Halagan M
AD  - Bioinformatics Research, Center for International Blood and Marrow Transplant 
      Research, Minneapolis, MN, USA.
FAU - Osoegawa, Kazutoyo
AU  - Osoegawa K
AD  - Stanford Blood Center, Palo Alto, CA, USA.
FAU - Fernandez-Vina, Marcelo
AU  - Fernandez-Vina M
AD  - Stanford Blood Center, Palo Alto, CA, USA; Stanford University School of 
      Medicine, Palo Alto, CA, USA.
LA  - eng
PT  - Journal Article
DEP - 20190923
PL  - United States
TA  - Hum Immunol
JT  - Human immunology
JID - 8010936
RN  - 0 (HLA Antigens)
SB  - IM
MH  - Computational Biology
MH  - Education
MH  - Family
MH  - Gene Frequency
MH  - *Genotype
MH  - HLA Antigens/*genetics
MH  - HapMap Project
MH  - Haplotypes
MH  - High-Throughput Nucleotide Sequencing/*methods
MH  - Histocompatibility Testing/methods
MH  - Humans
MH  - Immunogenetics
MH  - International Cooperation
MH  - Linkage Disequilibrium
MH  - Models, Biological
MH  - Pedigree
MH  - Polymorphism, Genetic
OTO - NOTNLM
OT  - Family
OT  - HLA haplotype
OT  - Linkage disequilibrium
EDAT- 2019/09/29 06:00
MHDA- 2020/04/04 06:00
CRDT- 2019/09/28 06:00
PHST- 2019/01/09 00:00 [received]
PHST- 2019/07/24 00:00 [revised]
PHST- 2019/07/25 00:00 [accepted]
PHST- 2019/09/29 06:00 [pubmed]
PHST- 2020/04/04 06:00 [medline]
PHST- 2019/09/28 06:00 [entrez]
AID - S0198-8859(19)31027-4 [pii]
AID - 10.1016/j.humimm.2019.07.298 [doi]
PST - ppublish
SO  - Hum Immunol. 2019 Nov;80(11):897-905. doi: 10.1016/j.humimm.2019.07.298. Epub 
      2019 Sep 23.