PMID- 31560392
OWN - NLM
STAT- MEDLINE
DCOM- 20200921
LR  - 20200921
IS  - 1537-6613 (Electronic)
IS  - 0022-1899 (Linking)
VI  - 221
IP  - 3
DP  - 2020 Jan 14
TI  - Identification of Naturally Processed Mumps Virus Epitopes by Mass Spectrometry: 
      Confirmation of Multiple CD8+ T-Cell Responses in Mumps Patients.
PG  - 474-482
LID - 10.1093/infdis/jiz480 [doi]
AB  - BACKGROUND: The re-emergence of mumps among vaccinated young adults has become a 
      global issue. Besides waning of antibody responses, suboptimal induction of 
      T-cell responses may reduce protection. In a recent study, we observed a dominant 
      polyfunctional CD8+ T-cell response after natural mumps virus (MuV) infection 
      that was not present after vaccination. Unraveling the MuV epitope repertoire can 
      provide insight in the specificity, functionality, and breadth of the T-cell 
      response against MuV. METHODS: Peptides were eluted from human leukocyte antigen 
      (HLA) class I molecules of MuV-infected cells and characterized by advanced mass 
      spectrometry. Selected identified MuV peptides were tested for in vitro and ex 
      vivo immunogenicity. RESULTS: In this study, we identified a broad landscape of 
      83 CD8+ T-cell epitopes of MuV, 41 of which were confirmed based on synthetic 
      peptide standards. For 6 epitopes, we showed induction of an HLA-A*02-restriced 
      CD8+ T-cell response. Moreover, robust T-cell responses against 5 selected MuV 
      epitopes could be detected in all tested mumps patients using peptide/HLA-A*02:01 
      dextramers. CONCLUSIONS: The identified CD8+ T-cell epitopes will help to further 
      characterize MuV-specific T-cell immunity after natural MuV infection or 
      vaccination. These MuV epitopes may provide clues for a better understanding of, 
      and possibly for preventing, mumps vaccine failure.We identified for the first 
      time 41 mumps virus (MuV)-specific HLA-A*02 epitopes. For 6 epitopes, CD8+ T-cell 
      responses were confirmed in T cells derived from several mumps cases, and 
      MuV-specific CD8+ T cells could be identified by peptide/dextramer staining.
CI  - (c) The Author(s) 2019. Published by Oxford University Press for the Infectious 
      Diseases Society of America.
FAU - de Wit, Jelle
AU  - de Wit J
AD  - Centre for Infectious Disease Control, National Institute for Public Health and 
      the Environment (RIVM), Bilthoven, The Netherlands.
FAU - Emmelot, Maarten E
AU  - Emmelot ME
AD  - Centre for Infectious Disease Control, National Institute for Public Health and 
      the Environment (RIVM), Bilthoven, The Netherlands.
FAU - Meiring, Hugo
AU  - Meiring H
AD  - Intravacc (Institute for Translational Vaccinology), Bilthoven, The Netherlands.
FAU - van Gaans-van den Brink, Jacqueline A M
AU  - van Gaans-van den Brink JAM
AD  - Centre for Infectious Disease Control, National Institute for Public Health and 
      the Environment (RIVM), Bilthoven, The Netherlands.
FAU - van Els, Cecile A C M
AU  - van Els CACM
AD  - Centre for Infectious Disease Control, National Institute for Public Health and 
      the Environment (RIVM), Bilthoven, The Netherlands.
FAU - Kaaijk, Patricia
AU  - Kaaijk P
AD  - Centre for Infectious Disease Control, National Institute for Public Health and 
      the Environment (RIVM), Bilthoven, The Netherlands.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Infect Dis
JT  - The Journal of infectious diseases
JID - 0413675
RN  - 0 (Epitopes, T-Lymphocyte)
RN  - 0 (HLA-A*02 antigen)
RN  - 0 (HLA-A2 Antigen)
RN  - 0 (IFNG protein, human)
RN  - 0 (Peptides)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - CD8-Positive T-Lymphocytes/*immunology
MH  - Cells, Cultured
MH  - Chromatography, Reverse-Phase/methods
MH  - Epitopes, T-Lymphocyte/chemistry/*immunology
MH  - Genotype
MH  - HLA-A2 Antigen/chemistry/immunology
MH  - Humans
MH  - Interferon-gamma/biosynthesis
MH  - Mumps/*immunology/pathology/virology
MH  - Mumps virus/genetics/*immunology
MH  - Peptides/chemistry/immunology
MH  - Tandem Mass Spectrometry/*methods
MH  - Young Adult
OTO - NOTNLM
OT  - MHC class I
OT  - MMR
OT  - T-cell immunity
OT  - immunopeptidome
OT  - mumps infection
EDAT- 2019/09/29 06:00
MHDA- 2020/09/22 06:00
CRDT- 2019/09/28 06:00
PHST- 2019/07/22 00:00 [received]
PHST- 2019/09/19 00:00 [accepted]
PHST- 2019/09/29 06:00 [pubmed]
PHST- 2020/09/22 06:00 [medline]
PHST- 2019/09/28 06:00 [entrez]
AID - 5575092 [pii]
AID - 10.1093/infdis/jiz480 [doi]
PST - ppublish
SO  - J Infect Dis. 2020 Jan 14;221(3):474-482. doi: 10.1093/infdis/jiz480.