PMID- 31560831
OWN - NLM
STAT- MEDLINE
DCOM- 20200626
LR  - 20231213
IS  - 1364-3703 (Electronic)
IS  - 1464-6722 (Print)
IS  - 1364-3703 (Linking)
VI  - 20
IP  - 12
DP  - 2019 Dec
TI  - Transcriptome reprogramming in the shoot apical meristem of CymRSV-infected 
      Nicotiana benthamiana plants associates with viral exclusion and the lack of 
      recovery.
PG  - 1748-1758
LID - 10.1111/mpp.12875 [doi]
AB  - In some plant-virus interactions plants show a sign of healing from virus 
      infection, a phenomenon called symptom recovery. It is assumed that the meristem 
      exclusion of the virus is essential to this process. The discovery of RNA 
      silencing provided a possible mechanism to explain meristem exclusion and 
      recovery. Here we show evidence that silencing is not the reason for meristem 
      exclusion in Nicotiana benthamiana plants infected with Cymbidium ringspot virus 
      (CymRSV). Transcriptome analysis followed by in situ hybridization shed light on 
      the changes in gene expression in the shoot apical meristem (SAM) on virus 
      infection. We observed the down-regulation of meristem-specific genes, including 
      WUSCHEL (WUS). However, WUS was not down-regulated in the SAM of plants infected 
      with meristem-invading viruses such as turnip vein-clearing virus (TVCV) and 
      cucumber mosaic virus (CMV). Moreover, there is no connection between loss of 
      meristem function and fast shoot necrosis since TVCV necrotized the shoot while 
      CMV did not. Our findings suggest that the observed transcriptional changes on 
      virus infection in the shoot are key factors in tip necrosis and symptom 
      recovery. We observed a lack of GLYCERALDEHYDE 3-PHOSPHATE DEHYDROGENASE (GAPDH) 
      expression in tissues around the meristem, which likely stops virus replication 
      and spread into the meristem.
CI  - (c) 2019 The Authors. Molecular Plant Pathology published by British Society for 
      Plant Pathology and John Wiley & Sons Ltd.
FAU - Medzihradszky, Anna
AU  - Medzihradszky A
AD  - Department of Plant Biotechnology, National Agricultural Research and Innovation 
      Centre, Szent-Gyorgyi Albert u. 4, Godollo, 2100, Hungary.
FAU - Gyula, Peter
AU  - Gyula P
AUID- ORCID: 0000-0002-3574-2827
AD  - Department of Plant Biotechnology, National Agricultural Research and Innovation 
      Centre, Szent-Gyorgyi Albert u. 4, Godollo, 2100, Hungary.
FAU - Sos-Hegedus, Anita
AU  - Sos-Hegedus A
AD  - Department of Plant Biotechnology, National Agricultural Research and Innovation 
      Centre, Szent-Gyorgyi Albert u. 4, Godollo, 2100, Hungary.
FAU - Szittya, Gyorgy
AU  - Szittya G
AD  - Department of Plant Biotechnology, National Agricultural Research and Innovation 
      Centre, Szent-Gyorgyi Albert u. 4, Godollo, 2100, Hungary.
FAU - Burgyan, Jozsef
AU  - Burgyan J
AD  - Department of Plant Biotechnology, National Agricultural Research and Innovation 
      Centre, Szent-Gyorgyi Albert u. 4, Godollo, 2100, Hungary.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190927
PL  - England
TA  - Mol Plant Pathol
JT  - Molecular plant pathology
JID - 100954969
RN  - EC 1.2.1.12 (Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating))
SB  - IM
MH  - Cucumovirus/*physiology
MH  - *Gene Expression Regulation, Plant
MH  - Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating)/metabolism
MH  - Meristem/virology
MH  - Plant Diseases/genetics/*virology
MH  - Plant Shoots
MH  - RNA Interference
MH  - Nicotiana/genetics/metabolism/*virology
MH  - *Transcriptome
PMC - PMC6859499
OTO - NOTNLM
OT  - Nicotiana benthamiana
OT  - CymRSV
OT  - shoot apical meristem
OT  - silencing
OT  - transcriptome
EDAT- 2019/09/29 06:00
MHDA- 2020/06/27 06:00
PMCR- 2019/09/27
CRDT- 2019/09/28 06:00
PHST- 2019/09/29 06:00 [pubmed]
PHST- 2020/06/27 06:00 [medline]
PHST- 2019/09/28 06:00 [entrez]
PHST- 2019/09/27 00:00 [pmc-release]
AID - MPP12875 [pii]
AID - 10.1111/mpp.12875 [doi]
PST - ppublish
SO  - Mol Plant Pathol. 2019 Dec;20(12):1748-1758. doi: 10.1111/mpp.12875. Epub 2019 
      Sep 27.