PMID- 31561059
OWN - NLM
STAT- MEDLINE
DCOM- 20200609
LR  - 20200609
IS  - 1096-0341 (Electronic)
IS  - 0042-6822 (Linking)
VI  - 538
DP  - 2019 Dec
TI  - Cytotoxic T lymphocyte epitopes identified from a contemporary strain of porcine 
      reproductive and respiratory syndrome virus enhance CD4+CD8+ T, CD8+ T, and gammadelta T 
      cell responses.
PG  - 35-44
LID - S0042-6822(19)30263-6 [pii]
LID - 10.1016/j.virol.2019.09.006 [doi]
AB  - Immuno-stimulatory class I-restricted cytotoxic T lymphocytes (CTL) epitopes of 
      porcine reproductive and respiratory syndrome virus (PRRSV) are important for 
      vaccine development. In this study we first determined the expression frequency 
      of swine leukocyte antigen (SLA) class I alleles in commercial pigs in the United 
      States. The SLA genotyping result allowed us to predict potential CTL epitopes 
      from a contemporary strain of PRRSV (RFLP 1-7-4) by using bioinformatic tools. 
      The predicted epitopes were then evaluated in an ex vivo stimulation assay with 
      peripheral blood mononuclear cells isolated from pigs experimentally-infected 
      with PRRSV. Using flow-cytometry analysis, we identified a number of 
      immuno-stimulatory CTL epitopes, including two peptides from GP3 and two from 
      Nsp9 that significantly improved both degranulation marker CD107a and IFN-gamma 
      production in cytotoxic CD4+CD8+ T cells, CD8+ T cells, and gammadelta T cells, and two 
      peptides that inhibited IFN-gamma production. These CTL epitopes will aid future 
      vaccine development against PRRSV.
CI  - Copyright (c) 2019 Elsevier Inc. All rights reserved.
FAU - Cao, Qian M
AU  - Cao QM
AD  - Department of Biomedical Sciences and Pathobiology, Virginia Maryland College of 
      Veterinary Medicine, Virginia Polytechnic Institute and State University, 
      Blacksburg, VA, USA.
FAU - Tian, Debin
AU  - Tian D
AD  - Department of Biomedical Sciences and Pathobiology, Virginia Maryland College of 
      Veterinary Medicine, Virginia Polytechnic Institute and State University, 
      Blacksburg, VA, USA.
FAU - Heffron, C Lynn
AU  - Heffron CL
AD  - Department of Biomedical Sciences and Pathobiology, Virginia Maryland College of 
      Veterinary Medicine, Virginia Polytechnic Institute and State University, 
      Blacksburg, VA, USA.
FAU - Subramaniam, Sakthivel
AU  - Subramaniam S
AD  - Department of Biomedical Sciences and Pathobiology, Virginia Maryland College of 
      Veterinary Medicine, Virginia Polytechnic Institute and State University, 
      Blacksburg, VA, USA.
FAU - Opriessnig, Tanja
AU  - Opriessnig T
AD  - The Roslin Institute and the Royal (Dick) School of Veterinary Studies, 
      University of Edinburgh, Midlothian, UK.
FAU - Foss, Dennis L
AU  - Foss DL
AD  - Zoetis Inc, Kalamazoo, MI, USA.
FAU - Calvert, Jay G
AU  - Calvert JG
AD  - Zoetis Inc, Kalamazoo, MI, USA.
FAU - Meng, Xiang-Jin
AU  - Meng XJ
AD  - Department of Biomedical Sciences and Pathobiology, Virginia Maryland College of 
      Veterinary Medicine, Virginia Polytechnic Institute and State University, 
      Blacksburg, VA, USA. Electronic address: xjmeng@vt.edu.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20190917
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (Epitopes, T-Lymphocyte)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - CD4-Positive T-Lymphocytes/*immunology
MH  - CD8-Positive T-Lymphocytes/*immunology
MH  - Epitopes, T-Lymphocyte/genetics/*immunology
MH  - Interferon-gamma/immunology
MH  - Leukocytes, Mononuclear/immunology
MH  - Porcine Reproductive and Respiratory Syndrome/genetics/*immunology/virology
MH  - Porcine respiratory and reproductive syndrome virus
MH  - Swine
MH  - T-Lymphocyte Subsets/*immunology
MH  - T-Lymphocytes, Cytotoxic/*immunology
OTO - NOTNLM
OT  - Cytotoxic T lymphocytes (CTL)
OT  - Immuno-stimulatory CTL epitopes
OT  - Porcine reproductive and respiratory syndrome virus (PRRSV)
OT  - Swine leukocyte antigen (SLA) class I
OT  - Vaccine
EDAT- 2019/09/29 06:00
MHDA- 2020/06/10 06:00
CRDT- 2019/09/28 06:00
PHST- 2019/07/07 00:00 [received]
PHST- 2019/09/12 00:00 [revised]
PHST- 2019/09/13 00:00 [accepted]
PHST- 2019/09/29 06:00 [pubmed]
PHST- 2020/06/10 06:00 [medline]
PHST- 2019/09/28 06:00 [entrez]
AID - S0042-6822(19)30263-6 [pii]
AID - 10.1016/j.virol.2019.09.006 [doi]
PST - ppublish
SO  - Virology. 2019 Dec;538:35-44. doi: 10.1016/j.virol.2019.09.006. Epub 2019 Sep 17.