PMID- 31564895
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220410
IS  - 1178-6930 (Print)
IS  - 1178-6930 (Electronic)
IS  - 1178-6930 (Linking)
VI  - 12
DP  - 2019
TI  - Atractylodes macrocephala polysaccharides regulate the innate immunity of 
      colorectal cancer cells by modulating the TLR4 signaling pathway.
PG  - 7111-7121
LID - 10.2147/OTT.S219623 [doi]
AB  - BACKGROUND: It has been well-recognized that the polysaccharides from 
      Atractylodes macrocephala (PAM) are immune system enhancers, which can facilitate 
      the proliferation of lymphocytes and stimulate immune cells. Nevertheless, the 
      antitumor effects of PAM and their molecular mechanisms remain unclear. AIM: Our 
      research aimed to evaluate the anti-cancer effects of PAM on colorectal cancer 
      (CRC). METHODS: We tested the effects of PAM on the growth and proliferation of 
      CRC cells and macrophages by MTT 
      (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. The 
      pro-inflammatory cytokines expression and secretion was analyzed by real-time 
      RT-PCR and ELISA assay. We also used MC38 cells xenograft model to test the 
      anti-cancer effects of PAM in vivo. RESULTS: We found that although PAM treatment 
      did not significantly affect the growth of CRC cells or enhance the proliferation 
      of bone marrow-derived macrophages (BMDMs), it could enhance the phagocytosis of 
      BMDMs by CRC cells. Biochemical tests and immunoblotting assays revealed that 
      exposing BMDMs to PAM promoted the production of interleukin-6 (IL-6), interferon 
      lambda (IFN lambda), tumor necrosis factor alpha (TNF-alpha), and nitric oxide (NO) through the 
      MyD88/TLR4-dependent signaling pathway. One noteworthy observation is that PAM 
      treatment could significantly prevent tumorigenesis of MC38 cells in C57BL/6J 
      mice and increase the survival duration of mice with tumors, without influence on 
      the weight of those mice. However, the anti-cancer effects of PAM were 
      compromised in TLR4 KO mice, further suggesting that TLR4 signaling plays a vital 
      role in the anti-cancer effects of PAM. CONCLUSION: Therefore, PAM may prove to 
      be a potential candidate in cancer immunotherapy.
CI  - (c) 2019 Feng et al.
FAU - Feng, Zifang
AU  - Feng Z
AD  - Department of Laboratory, Xingyi City People's Hospital, Xingyi, People's 
      Republic of China.
FAU - Yang, Ruibin
AU  - Yang R
AD  - Department of Laboratory, Xingyi City People's Hospital, Xingyi, People's 
      Republic of China.
FAU - Wu, Liusong
AU  - Wu L
AD  - Department of Pediatrics, Affiliated Hospital of Zunyi Medical University, Zunyi, 
      People's Republic of China.
FAU - Tang, Shihua
AU  - Tang S
AD  - Department of Laboratory, Xingyi City People's Hospital, Xingyi, People's 
      Republic of China.
FAU - Wei, Bin
AU  - Wei B
AD  - Department of Laboratory, Xingyi City People's Hospital, Xingyi, People's 
      Republic of China.
FAU - Guo, Lijia
AU  - Guo L
AD  - Department of Laboratory, Xingyi City People's Hospital, Xingyi, People's 
      Republic of China.
FAU - He, Ling
AU  - He L
AD  - Department of Laboratory, Xingyi City People's Hospital, Xingyi, People's 
      Republic of China.
FAU - Feng, Yonghuai
AU  - Feng Y
AD  - Department of Haematology, Affiliated Hospital of Zunyi Medical University, 
      Zunyi, People's Republic of China.
LA  - eng
PT  - Journal Article
DEP - 20190904
PL  - New Zealand
TA  - Onco Targets Ther
JT  - OncoTargets and therapy
JID - 101514322
PMC - PMC6733773
OTO - NOTNLM
OT  - TLR4
OT  - cancer immunotherapy
OT  - colorectal cancer
OT  - macrophages
OT  - polysaccharides from Atractylodes macrocephala
COIS- The authors declare that there are no conflicts of interest in this work.
EDAT- 2019/10/01 06:00
MHDA- 2019/10/01 06:01
PMCR- 2019/09/04
CRDT- 2019/10/01 06:00
PHST- 2019/06/15 00:00 [received]
PHST- 2019/08/01 00:00 [accepted]
PHST- 2019/10/01 06:00 [entrez]
PHST- 2019/10/01 06:00 [pubmed]
PHST- 2019/10/01 06:01 [medline]
PHST- 2019/09/04 00:00 [pmc-release]
AID - 219623 [pii]
AID - 10.2147/OTT.S219623 [doi]
PST - epublish
SO  - Onco Targets Ther. 2019 Sep 4;12:7111-7121. doi: 10.2147/OTT.S219623. eCollection 
      2019.