PMID- 31565549
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231014
IS  - 2167-8359 (Print)
IS  - 2167-8359 (Electronic)
IS  - 2167-8359 (Linking)
VI  - 7
DP  - 2019
TI  - lncRNA TMEM51-AS1 and RUSC1-AS1 function as ceRNAs for induction of laryngeal 
      squamous cell carcinoma and prediction of prognosis.
PG  - e7456
LID - 10.7717/peerj.7456 [doi]
LID - e7456
AB  - BACKGROUND: Long non-coding RNAs (lncRNAs) can function as competing endogenous 
      RNAs (ceRNAs) to interact with miRNAs to regulate target genes and promote cancer 
      initiation and progression. The expression of lncRNAs and miRNAs can be 
      epigenetically regulated. The goal of this study was to construct an 
      lncRNA-miRNA-mRNA ceRNA network in laryngeal squamous cell carcinoma (LSCC) and 
      reveal their methylation patterns, which was not investigated previously. 
      METHODS: Microarray datasets available from the Gene Expression Omnibus database 
      were used to identify differentially expressed lncRNAs (DELs), miRNAs (DEMs), and 
      genes (DEGs) between LSCC and controls, which were then overlapped with 
      differentially methylated regions (DMRs). The ceRNA network was established by 
      screening the interaction relationships between miRNAs and lncRNAs/mRNAs by 
      corresponding databases. TCGA database was used to identify prognostic 
      biomarkers. RESULTS: Five DELs (downregulated: TMEM51-AS1, SND1-IT1; upregulated: 
      HCP5, RUSC1-AS1, LINC00324) and no DEMs were overlapped with the DMRs, but only a 
      negative relationship occurred in the expression and methylation level of 
      TMEM51-AS1. Five DELs could interact with 11 DEMs to regulate 242 DEGs, which was 
      used to construct the ceRNA network, including TMEM51-AS1-miR-106b-SNX21/ 
      TRAPPC10, LINC00324/RUSC1-AS1-miR-16-SPRY4/MICAL2/ SLC39A14, 
      RUSC1-AS1-miR-10-SCG5 and RUSC1-AS1-miR-7-ZFP1 ceRNAs axes. Univariate Cox 
      regression analysis showed RUSC1-AS1 and SNX21 were associated with overall 
      survival (OS); LINC00324, miR-7 and ZFP1 correlated with recurrence-free survival 
      (RFS); miR-16, miR-10, SCG5, SPRY4, MICAL2 and SLC39A14 were both OS and 
      RFS-related. Furthermore, TRAPPC10 and SLC39A14 were identified as independent OS 
      prognostic factors by multivariate Cox regression analysis. CONCLUSION: DNA 
      methylation-mediated TMEM51-AS1 and non-methylation-mediated RUSC1-AS1 may 
      function as ceRNAs for induction of LSCC. They and their ceRNA axis genes 
      (particularly TMEM51-AS1-miR-106b-TRAPPC10; RUSC1-AS1-miR-16-SLC39A14) may be 
      potentially important prognostic biomarkers for LSCC.
CI  - (c)2019 Hui et al.
FAU - Hui, Lian
AU  - Hui L
AD  - Department of Otolaryngology, the First Hospital of China Medical University, 
      Shenyang, Liaoning Province, China.
FAU - Wang, Jing
AU  - Wang J
AD  - Department of Otolaryngology, the First Hospital of China Medical University, 
      Shenyang, Liaoning Province, China.
FAU - Zhang, Jialiang
AU  - Zhang J
AD  - Department of Otolaryngology, the First Hospital of China Medical University, 
      Shenyang, Liaoning Province, China.
FAU - Long, Jin
AU  - Long J
AD  - Department of General Surgery, the First Hospital of China Medical University, 
      Shenyang, Liaoning Province, China.
LA  - eng
PT  - Journal Article
DEP - 20190910
PL  - United States
TA  - PeerJ
JT  - PeerJ
JID - 101603425
PMC - PMC6743450
OTO - NOTNLM
OT  - Competing endogenous RNAs
OT  - Laryngeal squamous cell carcinoma
OT  - Long non-coding RNAs
OT  - Micrornas
OT  - Prognosis
COIS- The authors declare there are no competing interests.
EDAT- 2019/10/01 06:00
MHDA- 2019/10/01 06:01
PMCR- 2019/09/10
CRDT- 2019/10/01 06:00
PHST- 2019/03/11 00:00 [received]
PHST- 2019/07/10 00:00 [accepted]
PHST- 2019/10/01 06:00 [entrez]
PHST- 2019/10/01 06:00 [pubmed]
PHST- 2019/10/01 06:01 [medline]
PHST- 2019/09/10 00:00 [pmc-release]
AID - 7456 [pii]
AID - 10.7717/peerj.7456 [doi]
PST - epublish
SO  - PeerJ. 2019 Sep 10;7:e7456. doi: 10.7717/peerj.7456. eCollection 2019.