PMID- 31569343
OWN - NLM
STAT- MEDLINE
DCOM- 20200615
LR  - 20200615
IS  - 2073-4409 (Electronic)
IS  - 2073-4409 (Linking)
VI  - 8
IP  - 10
DP  - 2019 Sep 28
TI  - Dendritic Cells Expressing MyD88 Molecule Are Necessary and Sufficient for 
      CpG-Mediated Inhibition of IgE Production In Vivo.
LID - 10.3390/cells8101165 [doi]
LID - 1165
AB  - Elevated levels of immunoglobulin E (IgE) are associated with allergies and other 
      immunological disorders. Sensitization with alum adjuvant favours IgE production 
      while CpG-ODN adjuvant, a synthetic toll-like receptor 9 (TLR9) agonist, inhibits 
      it. The cellular mechanisms underlying in vivo TLR regulation of immunoglobulin 
      production, specially IgE, are still controversial. Specifically, TLR-mediated 
      IgE regulation in vivo is not yet known. In this study we showed that augmented 
      levels of IgE induced by sensitizations to OVA with or without alum adjuvant or 
      with OVA-pulsed dendritic cells (DCs) were inhibited by co-administration of CpG. 
      Notably, CpG-mediated suppression of IgE production required MyD88-expression on 
      DCs but not on B-cells. This finding contrasts with previous in vitro studies 
      reporting regulation of IgE by a direct action of CpG on B cells via MyD88 
      pathway. In addition, we showed that CpG also inhibited IgE production in a 
      MyD88-dependent manner when sensitization was performed with OVA-pulsed DCs. 
      Finally, CpG signalling through MyD88 pathway was also necessary and sufficient 
      to prevent anaphylactic antibody production involved in active cutaneous 
      anaphylaxis.
FAU - Alberca Custodio, Ricardo W
AU  - Alberca Custodio RW
AUID- ORCID: 0000-0002-3602-3306
AD  - Institute of Biomedical Sciences, Department of Immunology, University of Sao 
      Paulo, Sao Paulo 05508-000, Brazil. Ricardowesley@gmail.com.
FAU - Mirotti, Luciana
AU  - Mirotti L
AD  - Institute of Biomedical Sciences, Department of Immunology, University of Sao 
      Paulo, Sao Paulo 05508-000, Brazil. lmirotti@gmail.com.
FAU - Gomes, Eliane
AU  - Gomes E
AD  - Institute of Biomedical Sciences, Department of Immunology, University of Sao 
      Paulo, Sao Paulo 05508-000, Brazil. melloeag@gmail.com.
FAU - Nunes, Fernanda P B
AU  - Nunes FPB
AD  - Institute of Biomedical Sciences, Department of Immunology, University of Sao 
      Paulo, Sao Paulo 05508-000, Brazil.
FAU - S Vieira, Raquel
AU  - S Vieira R
AD  - Institute of Biomedical Sciences, Department of Immunology, University of Sao 
      Paulo, Sao Paulo 05508-000, Brazil. raqueldesouzavieira@outlook.com.
FAU - Graca, Luis
AU  - Graca L
AD  - Institute of Molecular Medicine, University of Lisbon, 1649-028 Lisbon, Portugal. 
      lgraca@medicina.ulisboa.pt.
FAU - R Almeida, Rafael
AU  - R Almeida R
AD  - Institute of Biomedical Sciences, Department of Immunology, University of Sao 
      Paulo, Sao Paulo 05508-000, Brazil. rafaelbio13@yahoo.com.br.
FAU - S Camara, Niels O
AU  - S Camara NO
AD  - Institute of Biomedical Sciences, Department of Immunology, University of Sao 
      Paulo, Sao Paulo 05508-000, Brazil. niels@icb.usp.br.
FAU - Russo, Momtchilo
AU  - Russo M
AD  - Institute of Biomedical Sciences, Department of Immunology, University of Sao 
      Paulo, Sao Paulo 05508-000, Brazil. momrusso@usp.br.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190928
PL  - Switzerland
TA  - Cells
JT  - Cells
JID - 101600052
RN  - 0 (Adjuvants, Immunologic)
RN  - 0 (CPG-oligonucleotide)
RN  - 0 (Myd88 protein, mouse)
RN  - 0 (Myeloid Differentiation Factor 88)
RN  - 0 (Oligodeoxyribonucleotides)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 9006-59-1 (Ovalbumin)
SB  - IM
MH  - Adjuvants, Immunologic/*administration & dosage/pharmacology
MH  - Animals
MH  - B-Lymphocytes/drug effects/immunology/metabolism
MH  - Dendritic Cells/drug effects/*immunology/metabolism
MH  - Disease Models, Animal
MH  - Female
MH  - Gene Expression Regulation/drug effects
MH  - Humans
MH  - Immunoglobulin E/drug effects/*metabolism
MH  - Mice
MH  - Myeloid Differentiation Factor 88/genetics/*metabolism
MH  - Oligodeoxyribonucleotides/*administration & dosage/pharmacology
MH  - Ovalbumin/*adverse effects
MH  - Respiratory Hypersensitivity/chemically induced/*drug therapy/metabolism
MH  - Signal Transduction/drug effects
PMC - PMC6829343
OTO - NOTNLM
OT  - IgE
OT  - IgG2c
OT  - allergy
OT  - anaphylaxis
OT  - dendritic cells
COIS- The authors declare no conflict of interest.
EDAT- 2019/10/02 06:00
MHDA- 2020/06/17 06:00
PMCR- 2019/10/01
CRDT- 2019/10/02 06:00
PHST- 2019/08/09 00:00 [received]
PHST- 2019/09/23 00:00 [revised]
PHST- 2019/09/25 00:00 [accepted]
PHST- 2019/10/02 06:00 [entrez]
PHST- 2019/10/02 06:00 [pubmed]
PHST- 2020/06/17 06:00 [medline]
PHST- 2019/10/01 00:00 [pmc-release]
AID - cells8101165 [pii]
AID - cells-08-01165 [pii]
AID - 10.3390/cells8101165 [doi]
PST - epublish
SO  - Cells. 2019 Sep 28;8(10):1165. doi: 10.3390/cells8101165.