PMID- 31570674
OWN - NLM
STAT- MEDLINE
DCOM- 20191017
LR  - 20191017
IS  - 1672-7347 (Print)
IS  - 1672-7347 (Linking)
VI  - 44
IP  - 8
DP  - 2019 Aug 28
TI  - [Immunohistochemical analysis for C3d, C4d, IgG, IgG4, and CD123 in diagnosis of 
      autoimmune skin diseases].
PG  - 878-884
LID - 10.11817/j.issn.1672-7347.2019.180800 [doi]
AB  - To assess the value of immunohistochemical analysis for expressions of C3d, C4d, 
      IgG, IgG4, and CD123 in the diagnosis of autoimmune skin diseases.
 Methods: We 
      investigated the expressions of C3d, C4d, IgG, IgG4, and CD123 in 
      paraffin-embedded, formalin-fixed tissues from 27 lupus erythematosus cases, 
      including 8 discoid lupus erythematosus (DLE) cases, 4 subacute cutaneous lupus 
      erythematosus (SCLE) cases, and 15 systemic lupus erythematosus (SLE) cases. 
      Tissues from 15 dermatomyositis (DM) cases, 15 bullous pemphigoid (BP) cases, and 
      15 pemphigus cases were examined by immunohistochemical analysis. The differences 
      in expression rates of C3d, C4d, IgG, IgG4, and CD123 between immunohistochemical 
      staining and direct immunofluorescence were compared in the diagnosis of these 
      diseases.
 Results: In the lupus erythematosus group, the positive rates of C3d 
      and C4d deposited along the dermoepidermal junction were 85.2% and 51.9%, 
      respectively. In the dermatomyositis group, the positive rates of C3d and C4d 
      deposited along the dermoepidermal junction were 40% and 0, respectively. The 
      expressions of C3d and C4d in lupus erythematosus tissues were significantly 
      higher than those in DM tissues (P<0.05). The expression of CD123 protein in skin 
      lesions of the lupus group was significantly higher than that in the DM group 
      (P<0.05). In the BP group, the positive rates of C3d and C4d deposited along the 
      dermoepidermal junction were 100% and 86.7%, respectively. In the pemphigus 
      group, the positive rates of C3d and C4d deposited in the intercellular space of 
      keratinocytes were 100% and 60%, respectively. The expressions of IgG and IgG4 in 
      pemphigus tissues were higher than those in BP tissues (P<0.05). And the ratios 
      of IgG4 to IgG in the pemphigus group was significantly higher than that in the 
      BP group (P<0.05).
 Conclusion: The assays of C3d and C4d define an important 
      diagnostic adjunct in evaluation of lupus erythematosus, BP and pemphigus. In 
      some cases, it may even replace the direct immunofluorescence as a diagnostic 
      adjunct. The expression of CD123 possesses certain clinical significance for the 
      differential diagnosis of lupus erythematosus, and IgG4 and IgG expressions have 
      adjunctive diagnostic significance for pemphigus.
FAU - Deng, Min
AU  - Deng M
AD  - Department of Dermatology, Second Xiangya Hospital, Central South University, 
      Changsha 410011, China.
FAU - Zhou, Xingyu
AU  - Zhou X
AD  - Department of Dermatology, Second Xiangya Hospital, Central South University, 
      Changsha 410011, China.
FAU - Zhang, Jing
AU  - Zhang J
AD  - Department of Dermatology, Second Xiangya Hospital, Central South University, 
      Changsha 410011, China.
FAU - Li, Yaping
AU  - Li Y
AD  - Department of Dermatology, Second Xiangya Hospital, Central South University, 
      Changsha 410011, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhong Nan Da Xue Xue Bao Yi Xue Ban
JT  - Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. 
      Medical sciences
JID - 101230586
RN  - 0 (Immunoglobulin G)
RN  - 0 (Interleukin-3 Receptor alpha Subunit)
RN  - 80295-45-0 (Complement C3d)
RN  - 9007-36-7 (Complement System Proteins)
SB  - IM
MH  - *Autoimmune Diseases
MH  - Complement C3d
MH  - Complement System Proteins
MH  - Humans
MH  - Immunoglobulin G
MH  - Interleukin-3 Receptor alpha Subunit
MH  - *Lupus Erythematosus, Systemic
MH  - *Pemphigus
EDAT- 2019/10/02 06:00
MHDA- 2019/10/18 06:00
CRDT- 2019/10/02 06:00
PHST- 2019/10/02 06:00 [entrez]
PHST- 2019/10/02 06:00 [pubmed]
PHST- 2019/10/18 06:00 [medline]
AID - 10.11817/j.issn.1672-7347.2019.180800 [doi]
PST - ppublish
SO  - Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2019 Aug 28;44(8):878-884. doi: 
      10.11817/j.issn.1672-7347.2019.180800.