PMID- 31571138
OWN - NLM
STAT- MEDLINE
DCOM- 20201104
LR  - 20210305
IS  - 1896-1851 (Electronic)
IS  - 1230-2821 (Linking)
VI  - 65
IP  - 1
DP  - 2020 Mar
TI  - GK1 Improves the Immune Response Induced by Dendritic Cells of BALB/c Mice 
      Infected with Leishmania mexicana Promastigotes.
PG  - 27-35
LID - 10.2478/s11686-019-00125-w [doi]
AB  - PURPOSE: Dendritic cells (DCs) are the most potent antigen-presenting cells 
      (APCs), and their capacity to activate the immune response has been widely used 
      in immunotherapies against different diseases, predominantly cancer. However, 
      they have not been so widely used in immunotherapies against infectious diseases. 
      Leishmania mexicana is the causative agent of cutaneous leishmaniasis in Mexico, 
      which can result in localized cutaneous leishmaniasis (LCL) and diffuse cutaneous 
      leishmaniasis (DCL). DCL is characterized by the incapability of the immune 
      response to control the parasite, which thus disseminates to all teguments. 
      Treatments against DCL have shown low efficacy, which is a reason why alternative 
      therapies such as immunotherapies are promising. One adjuvant that has proven its 
      effectiveness in immunotherapies against some cancers and infections is GK1, a 
      component of the SPVac vaccine against porcine cysticercosis. GK1 has the 
      capacity to elicit proinflammatory cytokines and chemokines from DCs and 
      macrophages. METHODS: We pulsed bone marrow-derived dendritic cells (BMDCs) with 
      GK1 and a lysate obtained from L. mexicana promastigotes and tested the efficacy 
      of this combination against the infection of susceptible mice with L. mexicana. 
      RESULTS: We found that BMDCs stimulated with GK1 and a lysate of L. mexicana 
      promastigotes secreted IFN-gamma and IL-12, and when they were adoptively transferred 
      to BALB/c mice which were then infected with L. mexicana promastigotes, there was 
      a reduction in the size of the lesion and in the parasite load. CONCLUSIONS: The 
      adjuvant properties of GK1 along with parasite antigens may have a protective 
      effect against the infection of BALB/c mice with L. mexicana.
FAU - Gutierrez-Kobeh, Laila
AU  - Gutierrez-Kobeh L
AD  - Unidad de Investigacion UNAM-INC, Division de Investigacion, Facultad de 
      Medicina, Universidad Nacional Autonoma de Mexico-Instituto Nacional de 
      Cardiologia "Ignacio Chavez", Juan Badiano No. 1, Col. Belisario Dominguez, 
      Seccion XVI, Delegacion Tlalpan, C.P. 14080, Mexico City, Mexico. 
      lgutierr@unam.mx.
FAU - Wilkins-Rodriguez, Arturo A
AU  - Wilkins-Rodriguez AA
AD  - Unidad de Investigacion UNAM-INC, Division de Investigacion, Facultad de 
      Medicina, Universidad Nacional Autonoma de Mexico-Instituto Nacional de 
      Cardiologia "Ignacio Chavez", Juan Badiano No. 1, Col. Belisario Dominguez, 
      Seccion XVI, Delegacion Tlalpan, C.P. 14080, Mexico City, Mexico.
LA  - eng
GR  - IN 225116/Direccion General de Asuntos del Personal Academico, Universidad 
      Nacional Autonoma de Mexico/
PT  - Journal Article
DEP - 20190930
PL  - Switzerland
TA  - Acta Parasitol
JT  - Acta parasitologica
JID - 9301947
RN  - 0 (Adjuvants, Immunologic)
RN  - 0 (Peptides, Cyclic)
RN  - 0 (Protozoan Proteins)
RN  - 0 (cyclo(cysteinyl-glycyl-aspargyl-seryl-aspargyl-prolyl-lysyl-seryl-cysteine))
RN  - 187348-17-0 (Interleukin-12)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Adjuvants, Immunologic/pharmacology
MH  - Adoptive Transfer
MH  - Animals
MH  - Dendritic Cells/*drug effects/*immunology
MH  - Interferon-gamma/immunology
MH  - Interleukin-12/immunology
MH  - Leishmania mexicana
MH  - Leishmaniasis, Cutaneous/immunology
MH  - Leishmaniasis, Diffuse Cutaneous/immunology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Parasite Load
MH  - Peptides, Cyclic/*immunology/*pharmacology
MH  - Protozoan Proteins/*immunology/pharmacology
OTO - NOTNLM
OT  - Dendritic cells
OT  - GK1
OT  - Leishmania mexicana
EDAT- 2019/10/02 06:00
MHDA- 2020/11/05 06:00
CRDT- 2019/10/02 06:00
PHST- 2019/06/04 00:00 [received]
PHST- 2019/09/17 00:00 [accepted]
PHST- 2019/10/02 06:00 [pubmed]
PHST- 2020/11/05 06:00 [medline]
PHST- 2019/10/02 06:00 [entrez]
AID - 10.2478/s11686-019-00125-w [pii]
AID - 10.2478/s11686-019-00125-w [doi]
PST - ppublish
SO  - Acta Parasitol. 2020 Mar;65(1):27-35. doi: 10.2478/s11686-019-00125-w. Epub 2019 
      Sep 30.