PMID- 31572003
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220410
IS  - 1179-1322 (Print)
IS  - 1179-1322 (Electronic)
IS  - 1179-1322 (Linking)
VI  - 11
DP  - 2019
TI  - Long Noncoding RNA GAS5 Acts As A Tumor Suppressor In Laryngeal Squamous Cell 
      Carcinoma Via miR-21.
PG  - 8487-8498
LID - 10.2147/CMAR.S213690 [doi]
AB  - PURPOSE: Long noncoding RNAs (lncRNAs) have been identified as an important class 
      of noncoding RNAs that are deeply involved in multiple biological processes in 
      tumorigenesis. This study is to investigate the critical roles and biological 
      function of lncRNA growth arrest-specific 5 (GAS5) in tumorigenesis of laryngeal 
      squamous cell carcinoma (LSCC). PATIENTS AND METHODS: A total of 59 samples of 
      LSCC and paired adjacent tissue, as well as corresponding clinicopathological 
      information were collected. GAS5 expression in both LSCC tissues and human 
      SUN1076 and SNU899 cell lines were analyzed by Real-time quantitative RT-PCR 
      method. Ectopic expression of GAS5 by vector transfection in LSCC cell lines and 
      followed by in vitro experiments was to investigate the critical roles and 
      function of GAS5 in LSCC. Cell Counting Kit 8 (CCK8) assay and PE/7AAD Annexin V 
      Apoptosis analysis was to evaluate cell proliferation ability and cell apoptosis. 
      Co-transfection of GAS5 and miR-21 was to explore the interaction between GAS5 
      and miR-21 in LSCC. BAX and CDK6 protein level were analyzed by western blot 
      method. RESULTS: This study demonstrated that GAS5 was significantly 
      downregulated in LSCC tissue and human LSCC cell lines. GAS5 levels were 
      correlated with the clinicopathological features of LSCC patients. In addition, 
      the ectopic expression of GAS5 significantly inhibited cell proliferation and 
      promoted apoptosis. Co-expression analyses indicated that GAS5 is negatively 
      correlated with miR-21 in LSCC tissues. Overexpression of miR-21 eliminated 
      GAS5-mediated cell apoptosis and proliferation suppression. Furthermore, GAS5, 
      which upregulated BAX mRNA expression and downregulated CDK6 mRNA expression, was 
      reversed by ectopic expression of miR-21. CONCLUSION: GAS5 suppresses LSCC 
      progression through the negative regulation of miR-21 and its targets involved in 
      cell proliferation and apoptosis, indicating that GAS5 may serve as a biomarker 
      and potential target for LSCC therapy.
CI  - (c) 2019 Lyu et al.
FAU - Lyu, Kexing
AU  - Lyu K
AD  - Department of Otolaryngology, The First Affiliated Hospital of Sun Yat-Sen 
      University, Guangzhou, People's Republic of China.
FAU - Xu, Yang
AU  - Xu Y
AD  - Department of Otolaryngology, The First Affiliated Hospital of Sun Yat-Sen 
      University, Guangzhou, People's Republic of China.
FAU - Yue, Huijun
AU  - Yue H
AD  - Department of Otolaryngology, The First Affiliated Hospital of Sun Yat-Sen 
      University, Guangzhou, People's Republic of China.
FAU - Li, Yun
AU  - Li Y
AD  - Department of Otolaryngology, The First Affiliated Hospital of Sun Yat-Sen 
      University, Guangzhou, People's Republic of China.
FAU - Zhao, Jing
AU  - Zhao J
AD  - Department of Otolaryngology, The Third Hospital of Heibei Medical University, 
      Shijiazhuang, People's Republic of China.
FAU - Chen, Lin
AU  - Chen L
AD  - Department of Otolaryngology, The First Affiliated Hospital of Sun Yat-Sen 
      University, Guangzhou, People's Republic of China.
FAU - Wu, Jianhui
AU  - Wu J
AD  - Department of Otolaryngology, Meizhou People's Hospital, Meizhou, People's 
      Republic of China.
FAU - Zhu, Xiaolin
AU  - Zhu X
AD  - Department of Otolaryngology, The First Affiliated Hospital of Sun Yat-Sen 
      University, Guangzhou, People's Republic of China.
FAU - Chai, Liping
AU  - Chai L
AD  - Department of Otolaryngology, The First Affiliated Hospital of Sun Yat-Sen 
      University, Guangzhou, People's Republic of China.
FAU - Li, Chunwei
AU  - Li C
AD  - Department of Otolaryngology, The First Affiliated Hospital of Sun Yat-Sen 
      University, Guangzhou, People's Republic of China.
FAU - Wen, Weiping
AU  - Wen W
AD  - Department of Otolaryngology, The First Affiliated Hospital of Sun Yat-Sen 
      University, Guangzhou, People's Republic of China.
FAU - Lei, Wenbin
AU  - Lei W
AD  - Department of Otolaryngology, The First Affiliated Hospital of Sun Yat-Sen 
      University, Guangzhou, People's Republic of China.
LA  - eng
PT  - Journal Article
DEP - 20190917
PL  - New Zealand
TA  - Cancer Manag Res
JT  - Cancer management and research
JID - 101512700
EIN - Cancer Manag Res. 2019 Oct 23;11:9047. PMID: 31695496
PMC - PMC6756574
OTO - NOTNLM
OT  - GAS5
OT  - LSCC
OT  - apoptosis
OT  - long noncoding RNA
OT  - miR-21
OT  - proliferation
COIS- The authors report no conflicts of interest in this work.
EDAT- 2019/10/02 06:00
MHDA- 2019/10/02 06:01
PMCR- 2019/09/17
CRDT- 2019/10/02 06:00
PHST- 2019/04/27 00:00 [received]
PHST- 2019/09/04 00:00 [accepted]
PHST- 2019/10/02 06:00 [entrez]
PHST- 2019/10/02 06:00 [pubmed]
PHST- 2019/10/02 06:01 [medline]
PHST- 2019/09/17 00:00 [pmc-release]
AID - 213690 [pii]
AID - 10.2147/CMAR.S213690 [doi]
PST - epublish
SO  - Cancer Manag Res. 2019 Sep 17;11:8487-8498. doi: 10.2147/CMAR.S213690. 
      eCollection 2019.