PMID- 31572345
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240214
IS  - 1664-302X (Print)
IS  - 1664-302X (Electronic)
IS  - 1664-302X (Linking)
VI  - 10
DP  - 2019
TI  - Niche Differentiation of Aerobic and Anaerobic Ammonia Oxidizers in a High 
      Latitude Deep Oxygen Minimum Zone.
PG  - 2141
LID - 10.3389/fmicb.2019.02141 [doi]
LID - 2141
AB  - To elucidate the potential for nitrification and denitrification processes in a 
      high latitude deep oxygen minimum zone (OMZ) we determined the abundance and 
      community composition of the main microbial players in the aerobic and anaerobic 
      (anammox) ammonium oxidation and denitrification processes in the Gulf of Alaska 
      throughout the water column. Within the dominant bacterial groups, 
      Flavobacterales, Rhodobacterales, Actinomarinales, and SAR86 were more abundant 
      in epipelagic waters and decreased with depth, whereas SAR11, SAR324, 
      Marinimicrobia, and Thiomicrospirales increased their contribution to the 
      bacterial community with depth. Nitrosopumilaceae also increased with depth and 
      dominated the OMZ and bathypelagic archaeal communities. Euryarchaeota Marine 
      Group II exhibited an opposite depth pattern to Nitrosopumilaceae, whereas Marine 
      Group III and Woesearchaeota were more abundant in the bathypelagic realm. 
      Candidatus Brocadia contributed 70-100% of the anammox bacterial community 
      throughout the water column. Archaeal ammonia oxidizers (AOA) dominated the 
      microbial community involved in the nitrogen cycle. Two AOA ecotypes, the high 
      ammonia (HAC) and low ammonia (LAC)-AOA, characterized by distinct genes for 
      aerobic ammonia oxidation (amoA) and for denitrification (nirK), exhibited a 
      distinct distribution pattern related to depth and ammonia concentrations. 
      HAC-AOA dominated in epipelagic (80.5 +/- 28.3% of total AOA) oxygenated and 
      ammonia-rich waters, and LAC-AOA dominated in the OMZ (90.9 +/- 5.1%) and 
      bathypelagic waters (85.5 +/- 13.5%), characterized by lower oxygen and ammonia 
      concentrations. Bacterial denitrifiers (3.7 +/- 6.9 bacterial nirK gene mL(-1)) and 
      anaerobic ammonia oxidizers (78 +/- 322 anammox 16S rRNA genes L(-1)) were low in 
      abundance under the oxygen conditions in the Gulf of Alaska throughout the water 
      column. The widespread distribution of bacterial denitrifiers and anaerobic 
      ammonia oxidizers in low abundances reveals a reservoir of genetic and metabolic 
      potential ready to colonize the environment under the predicted increase of OMZs 
      in the ocean. Taken together, our results reinforce the niche partitioning of 
      archaeal ammonia oxidizers based on their distinct metabolic characteristics 
      resulting in the dominance of LAC-AOA in a high latitude deep OMZ. Considering 
      the different ecological roles and functions of the two archaeal ecotypes, the 
      expansion of the zones dominated by the LAC-ecotype might have implications for 
      the nitrogen cycle in the future ocean.
CI  - Copyright (c) 2019 Muck, De Corte, Clifford, Bayer, Herndl and Sintes.
FAU - Muck, Simone
AU  - Muck S
AD  - Department of Limnology and Bio-Oceanography, Center of Functional Ecology, 
      University of Vienna, Vienna, Austria.
AD  - NIOZ, Department of Marine Microbiology and Biogeochemistry, Royal Netherlands 
      Institute for Sea Research, Utrecht University, Den Burg, Netherlands.
FAU - De Corte, Daniele
AU  - De Corte D
AD  - Research and Development Center for Marine Biosciences, Japan Agency for 
      Marine-Earth Science and Technology (JAMSTEC), Yokosuka, Japan.
FAU - Clifford, Elisabeth L
AU  - Clifford EL
AD  - Department of Limnology and Bio-Oceanography, Center of Functional Ecology, 
      University of Vienna, Vienna, Austria.
FAU - Bayer, Barbara
AU  - Bayer B
AD  - Department of Limnology and Bio-Oceanography, Center of Functional Ecology, 
      University of Vienna, Vienna, Austria.
FAU - Herndl, Gerhard J
AU  - Herndl GJ
AD  - Department of Limnology and Bio-Oceanography, Center of Functional Ecology, 
      University of Vienna, Vienna, Austria.
AD  - NIOZ, Department of Marine Microbiology and Biogeochemistry, Royal Netherlands 
      Institute for Sea Research, Utrecht University, Den Burg, Netherlands.
FAU - Sintes, Eva
AU  - Sintes E
AD  - Department of Limnology and Bio-Oceanography, Center of Functional Ecology, 
      University of Vienna, Vienna, Austria.
AD  - Ecosystem Oceanography Group (GRECO), Instituto Espanol de Oceanografia, Centro 
      Oceanografico de Baleares, Palma, Spain.
LA  - eng
GR  - I 486/FWF_/Austrian Science Fund FWF/Austria
PT  - Journal Article
DEP - 20190913
PL  - Switzerland
TA  - Front Microbiol
JT  - Frontiers in microbiology
JID - 101548977
PMC - PMC6753893
OTO - NOTNLM
OT  - Gulf of Alaska
OT  - OMZ
OT  - ammonia oxidizers
OT  - anammox
OT  - archaea
OT  - denitrifiers
EDAT- 2019/10/02 06:00
MHDA- 2019/10/02 06:01
PMCR- 2019/09/13
CRDT- 2019/10/02 06:00
PHST- 2018/12/10 00:00 [received]
PHST- 2019/08/30 00:00 [accepted]
PHST- 2019/10/02 06:00 [entrez]
PHST- 2019/10/02 06:00 [pubmed]
PHST- 2019/10/02 06:01 [medline]
PHST- 2019/09/13 00:00 [pmc-release]
AID - 10.3389/fmicb.2019.02141 [doi]
PST - epublish
SO  - Front Microbiol. 2019 Sep 13;10:2141. doi: 10.3389/fmicb.2019.02141. eCollection 
      2019.