PMID- 31572594
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231014
IS  - 2048-7177 (Print)
IS  - 2048-7177 (Electronic)
IS  - 2048-7177 (Linking)
VI  - 7
IP  - 9
DP  - 2019 Sep
TI  - Comparison of three different lipid removal cleanup techniques prior to the 
      analysis of sulfonamide drug residues in porcine tissues.
PG  - 3006-3016
LID - 10.1002/fsn3.1158 [doi]
AB  - A number of 17 sulfonamides (SNs) determination in porcine tissues using two new 
      materials including Enhanced Matrix Removal for Lipid (EMR-L) and Oasis PRiME 
      hydrophilic-lipophilic balance (HLB), and the conventional liquid-liquid 
      extraction with n-hexane (LLE) sample preparation methods were evaluated and 
      compared. Samples were extracted uniformly with acidified acetonitrile and 
      cleaned up by the three sample preparation methods, and then, analytes were 
      further separated by ultrahigh-performance liquid chromatography (UHPLC) and 
      detected by a triple quadrupole mass spectrometry (MS/MS) or a 
      quadrupole-time-of-flight tandem mass spectrometry (Q-TOF/MS). The results showed 
      that the matrix effects from the EMR-L and HLB were significantly lower than that 
      from LLE, suggesting that these two new materials are superior to n-hexane in the 
      precipitation of proteins and the adsorption of lipids. Moreover, the recoveries 
      of 17 SNs were quantified by the matrix-matched calibration curve at spiked level 
      of 5, 10, and 20 mug/kg, and 97.0% of the results satisfied method validation 
      requirements. The samples cleaned up by EMR-L and HLB achieved the highest 
      average recovery in liver and kidney with high moisture content, and muscle which 
      is high in fat, respectively. In addition, Q-TOF/MS could play a good role in 
      aided verification based on the result of repeated validation test. In summary, 
      either combination of approaches could be used to achieve monitoring purposes; it 
      is still worthwhile to adopt a specific sample preparation method and MS detector 
      for the quantification in a specific matrix.
CI  - (c) 2019 The Authors. Food Science & Nutrition published by Wiley Periodicals, Inc.
FAU - Wang, Jie
AU  - Wang J
AD  - College of Food Science and Engineering Nanjing University of Finance and 
      Economics Nanjing China.
FAU - Hu, Qiuhui
AU  - Hu Q
AD  - College of Food Science and Engineering Nanjing University of Finance and 
      Economics Nanjing China.
FAU - Li, Peng
AU  - Li P
AD  - College of Food Science and Engineering Nanjing University of Finance and 
      Economics Nanjing China.
FAU - Fang, Yong
AU  - Fang Y
AD  - College of Food Science and Engineering Nanjing University of Finance and 
      Economics Nanjing China.
FAU - Yang, Wenjian
AU  - Yang W
AD  - College of Food Science and Engineering Nanjing University of Finance and 
      Economics Nanjing China.
FAU - Ma, Ning
AU  - Ma N
AD  - College of Food Science and Engineering Nanjing University of Finance and 
      Economics Nanjing China.
FAU - Pei, Fei
AU  - Pei F
AUID- ORCID: 0000-0001-5063-6339
AD  - College of Food Science and Engineering Nanjing University of Finance and 
      Economics Nanjing China.
LA  - eng
PT  - Journal Article
DEP - 20190807
PL  - United States
TA  - Food Sci Nutr
JT  - Food science & nutrition
JID - 101605473
PMC - PMC6766571
OTO - NOTNLM
OT  - enhanced matrix removal for lipid
OT  - hydrophilic-lipophilic balance
OT  - mass spectrometry
OT  - porcine tissues
OT  - sulfonamides
COIS- No conflict of interest declared.
EDAT- 2019/10/02 06:00
MHDA- 2019/10/02 06:01
PMCR- 2019/08/07
CRDT- 2019/10/02 06:00
PHST- 2019/05/05 00:00 [received]
PHST- 2019/06/20 00:00 [revised]
PHST- 2019/06/25 00:00 [accepted]
PHST- 2019/10/02 06:00 [entrez]
PHST- 2019/10/02 06:00 [pubmed]
PHST- 2019/10/02 06:01 [medline]
PHST- 2019/08/07 00:00 [pmc-release]
AID - FSN31158 [pii]
AID - 10.1002/fsn3.1158 [doi]
PST - epublish
SO  - Food Sci Nutr. 2019 Aug 7;7(9):3006-3016. doi: 10.1002/fsn3.1158. eCollection 
      2019 Sep.