PMID- 3157451
OWN - NLM
STAT- MEDLINE
DCOM- 19850619
LR  - 20131121
IS  - 0008-5472 (Print)
IS  - 0008-5472 (Linking)
VI  - 45
IP  - 6
DP  - 1985 Jun
TI  - Endogenous concentration and subcellular distribution of androgens in normal and 
      malignant human breast tissue.
PG  - 2907-12
AB  - The endogenous concentrations and the subcellular distribution of 
      dehydroepiandrosterone (DHEA) and 5-androstene-3 beta,17 beta-diol (ADIOL) were 
      measured in malignant and nonmalignant human breast tissue from both pre- and 
      postmenopausal women. DHEA 3-sulfate was measured only in the cytosol. A greater 
      tissue-plasma gradient of DHEA was present with large variations. The highest 
      concentration of DHEA and ADIOL occurred in the nuclear fraction (average, 2.9 
      and 1.6 times the concentration in cytosol). With respect to DHEA, this finding 
      is remarkable because no specific binding protein in human breast tissue has been 
      reported. The concentration of DHEA 3-sulfate was significantly higher in the 
      cytosol of nonmalignant than in malignant breast tissues. No significant 
      differences in tissue concentrations of DHEA and ADIOL were found in malignant 
      and nonmalignant breast tissue. The concentration of estrogens was measured in 
      the cytosol and the nuclear fraction of the same tissues, as reported in a 
      previous paper. We found a significantly higher estradiol concentration in 
      malignant tissue as compared to nonmalignant tissue. When the ratio of ADIOL to 
      estradiol was calculated from the combined data, a significant difference was 
      found only in the cytosol of premenopausal cancer patients versus normal women. 
      No difference was seen in the postmenopausal women. No difference in the 
      ADIOL:estradiol ratio was found between normal and malignant breast tissue of 
      patients of the same menopausal status.
FAU - van Landeghem, A A
AU  - van Landeghem AA
FAU - Poortman, J
AU  - Poortman J
FAU - Nabuurs, M
AU  - Nabuurs M
FAU - Thijssen, J H
AU  - Thijssen JH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Res
JT  - Cancer research
JID - 2984705R
RN  - 0 (Androgens)
RN  - 459AG36T1B (Dehydroepiandrosterone)
RN  - 4TI98Z838E (Estradiol)
RN  - 95PS51EMXY (Androstenediol)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Age Factors
MH  - Aged
MH  - Androgens/*analysis/blood
MH  - Androstenediol/analysis
MH  - Breast/*analysis
MH  - Breast Neoplasms/*analysis
MH  - Cell Nucleus/analysis
MH  - Cytosol/analysis
MH  - Dehydroepiandrosterone/analysis
MH  - Estradiol/analysis
MH  - Female
MH  - Humans
MH  - Menopause
MH  - Middle Aged
EDAT- 1985/06/01 00:00
MHDA- 1985/06/01 00:01
CRDT- 1985/06/01 00:00
PHST- 1985/06/01 00:00 [pubmed]
PHST- 1985/06/01 00:01 [medline]
PHST- 1985/06/01 00:00 [entrez]
PST - ppublish
SO  - Cancer Res. 1985 Jun;45(6):2907-12.