PMID- 31575081
OWN - NLM
STAT- MEDLINE
DCOM- 20200210
LR  - 20231014
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 20
IP  - 19
DP  - 2019 Sep 30
TI  - Human Leukocyte Antigen and Red Blood Cells Impact Umbilical Cord Blood CD34(+) 
      Cell Viability after Thawing.
LID - 10.3390/ijms20194875 [doi]
LID - 4875
AB  - Hematopoietic progenitor cell (HPC) transplantation is a treatment option for 
      malignant and nonmalignant diseases. Umbilical cord blood (UCB) is an important 
      HPC source, mainly for pediatric patients. It has been demonstrated that human 
      leukocyte antigen (HLA) matching and cell dose are the most important features 
      impacting clinical outcomes. However, UCB matching is performed using low 
      resolution HLA typing and it has been demonstrated that the unnoticed mismatches 
      negatively impact the transplant. Since we found differences in CD34(+) viability 
      after thawing of UCB units matched for two different patients (p = 0.05), we 
      presumed a possible association between CD34(+) cell viability and HLA. We 
      performed a multivariate linear model (n = 67), comprising pre-cryopreservation 
      variables and high resolution HLA genotypes separately. We found that 
      pre-cryopreservation red blood cells (RBC), granulocytes, and viable CD34(+) cell 
      count significantly impacted CD34(+) viability after thawing, along with HLA-B or 
      -C (R(2) = 0.95, p = 0.01; R(2) = 0.56, p = 0.007, respectively). Although 
      HLA-B*40:02 may have a negative impact on CD34(+) cell viability, RBC depletion 
      significantly improves it.
FAU - Vanegas, Diana
AU  - Vanegas D
AD  - Specialized researcher, Cord Blood Bank, Instituto Distrital de Ciencia, 
      Biotecnologia e Innovacion en Salud. Cra. 32 # 12-81, 111611 Bogota, Colombia. 
      dmvanegas@idcbis.org.co.
FAU - Galindo, Cristian-Camilo
AU  - Galindo CC
AD  - Specialized researcher, Cord Blood Bank, Instituto Distrital de Ciencia, 
      Biotecnologia e Innovacion en Salud. Cra. 32 # 12-81, 111611 Bogota, Colombia. 
      ccgalindoun@gmail.com.
FAU - Paez-Gutierrez, Ivan-Aurelio
AU  - Paez-Gutierrez IA
AD  - Specialized researcher, Cord Blood Bank, Instituto Distrital de Ciencia, 
      Biotecnologia e Innovacion en Salud. Cra. 32 # 12-81, 111611 Bogota, Colombia. 
      ipaez@idcbis.org.co.
FAU - Gonzalez-Acero, Lorena-Xiomara
AU  - Gonzalez-Acero LX
AD  - Specialized researcher, Cord Blood Bank, Instituto Distrital de Ciencia, 
      Biotecnologia e Innovacion en Salud. Cra. 32 # 12-81, 111611 Bogota, Colombia. 
      lxgonzalez@idcbis.org.co.
FAU - Medina-Valderrama, Pavel-Tiberio
AU  - Medina-Valderrama PT
AD  - Specialized researcher, Cord Blood Bank, Instituto Distrital de Ciencia, 
      Biotecnologia e Innovacion en Salud. Cra. 32 # 12-81, 111611 Bogota, Colombia. 
      ptmedina@idcbis.org.co.
FAU - Lozano, Juan-Camilo
AU  - Lozano JC
AD  - Specialized researcher, Cord Blood Bank, Instituto Distrital de Ciencia, 
      Biotecnologia e Innovacion en Salud. Cra. 32 # 12-81, 111611 Bogota, Colombia. 
      jclozanoc@unal.edu.co.
FAU - Camacho-Rodriguez, Bernardo
AU  - Camacho-Rodriguez B
AD  - Director, Instituto Distrital de Ciencia, Biotecnologia e Innovacion en Salud. 
      Cra. 32 # 12-81, 111611 Bogota, Colombia. bacamacho@idcbis.org.co.
FAU - Perdomo-Arciniegas, Ana-Maria
AU  - Perdomo-Arciniegas AM
AD  - Scientific leader, Cord Blood Bank, Instituto Distrital de Ciencia, Biotecnologia 
      e Innovacion en Salud. Cra. 32 # 12-81, 111611 Bogota, Colombia. 
      amperdomo@idcbis.org.co.
LA  - eng
GR  - BPIN2016000100035/Sistema General de Regalias. Fondo de CTeI, Colombia. 
      Secretaria Distrital de Salud, Bogota DC, Colombia./
PT  - Journal Article
DEP - 20190930
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Antigens, CD34)
RN  - 0 (HLA Antigens)
SB  - IM
MH  - Alleles
MH  - Antigens, CD34/metabolism
MH  - *Cell Communication/genetics
MH  - Cell Survival/genetics
MH  - Cord Blood Stem Cell Transplantation
MH  - Cryopreservation
MH  - Erythrocytes/*metabolism
MH  - Fetal Blood/*cytology
MH  - HLA Antigens/*genetics
MH  - Hematopoietic Stem Cells/cytology/*metabolism
MH  - Humans
PMC - PMC6801469
OTO - NOTNLM
OT  - cell viability
OT  - hematopoietic stem cell transplantation
OT  - human leukocyte antigen
OT  - thawing
OT  - umbilical cord blood
COIS- The authors declare no conflict of interest.
EDAT- 2019/10/03 06:00
MHDA- 2020/02/11 06:00
PMCR- 2019/10/01
CRDT- 2019/10/03 06:00
PHST- 2019/08/29 00:00 [received]
PHST- 2019/09/21 00:00 [revised]
PHST- 2019/09/25 00:00 [accepted]
PHST- 2019/10/03 06:00 [entrez]
PHST- 2019/10/03 06:00 [pubmed]
PHST- 2020/02/11 06:00 [medline]
PHST- 2019/10/01 00:00 [pmc-release]
AID - ijms20194875 [pii]
AID - ijms-20-04875 [pii]
AID - 10.3390/ijms20194875 [doi]
PST - epublish
SO  - Int J Mol Sci. 2019 Sep 30;20(19):4875. doi: 10.3390/ijms20194875.