PMID- 31576093
OWN - NLM
STAT- MEDLINE
DCOM- 20200226
LR  - 20200226
IS  - 2219-2840 (Electronic)
IS  - 1007-9327 (Print)
IS  - 1007-9327 (Linking)
VI  - 25
IP  - 36
DP  - 2019 Sep 28
TI  - Clostridium butyricum alleviates intestinal low-grade inflammation in 
      TNBS-induced irritable bowel syndrome in mice by regulating functional status of 
      lamina propria dendritic cells.
PG  - 5469-5482
LID - 10.3748/wjg.v25.i36.5469 [doi]
AB  - BACKGROUND: Irritable bowel syndrome (IBS) is one of the most common functional 
      gas-troenterological diseases characterized by abnormal visceral sensitivity and 
      low-grade inflammation. The role of Clostridium butyricum (C. butyricum) in 
      reducing intestinal low-grade inflammation via immune pathways has been well 
      defined. However, the detailed mechanisms of the effects of C. butyricum on 
      intestinal mucosal immunity, especially on immune cells of the lamina propria, 
      remain unclear. Dendritic cells (DCs), which are important immune cells, secrete 
      proinflammatory cytokines (IL-1beta, IL-6, and others) and express T cell 
      immuno-globulin and mucin domain-3 (TIM3), promoting proliferation and activation 
      of DCs, and mediating Th1 and Th17 inflammatory responses. AIM: To investigate 
      the role of DCs in the development of IBS in a rat model and to understand the 
      regulation of DCs after C. butyricum intervention. METHODS: An IBS animal model 
      was established using C57BL/6 mice, and C. butyricum was continuously 
      administered via the intragastric route to simulate different intestinal immune 
      states. Intestinal visceral hypersensitivity and histopathology were assessed 
      using the abdominal withdrawal reflex (AWR) test and hematoxylin & eosin (H&E) 
      staining, respectively. The expression of proinflammatory cytokines (IL-1beta and 
      IL-6) and TIM3 was analyzed by Western blot analysis and real-time PCR. Flow 
      cytometry was applied to analyze the quantity, function, and membrane molecule 
      TIM3 of the lamina propria dendritic cells (LPDCs). The regulatory effect of C. 
      butyricum was verified in bone marrow-derived dendritic cells by in vitro 
      experiments. RESULTS: The secretion of proinflammatory cytokines (IL-1beta and IL-6) 
      in mice with IBS was significantly increased compared with that of the control 
      group, which suggested that the intestinal mucosa in mice with IBS was in a 
      low-grade inflammatory state. The expression of CD11C+CD80+ and CD11c+TIM3+ in 
      intestinal LPDCs in mice with IBS increased significantly. Meanwhile, the 
      cytokines (IL-1beta and IL-6) were significantly reduced after the intervention with 
      probiotic C. butyricum. The amount and function of LPDCs and the TIM3 on the 
      surface of the LPDCs were decreased with the alleviation of the intestinal 
      inflammatory response. CONCLUSION: The results suggest that C. butyricum 
      regulates the amount and functional status of LPDCs in the intestinal mucosa of 
      mice with IBS, and therefore modulates the local immune response in the 
      intestine.
CI  - (c)The Author(s) 2019. Published by Baishideng Publishing Group Inc. All rights 
      reserved.
FAU - Zhao, Qin
AU  - Zhao Q
AD  - Department of Gastroenterology, Qilu Hospital, Shandong University, Jinan 250012, 
      Shandong Province, China.
FAU - Yang, Wen-Rong
AU  - Yang WR
AD  - Department of Clinical Nutrition, Taian City Central Hospital, Taian 271000, 
      Shandong Province, China.
FAU - Wang, Xiao-Hong
AU  - Wang XH
AD  - Department of Gastroenterology, Taian City Central Hospital, Taian 271000, 
      Shandong Province, China.
FAU - Li, Gai-Qin
AU  - Li GQ
AD  - Department of Gastroenterology, Taian City Central Hospital, Taian 271000, 
      Shandong Province, China.
FAU - Xu, Lei-Qi
AU  - Xu LQ
AD  - Department of Gastroenterology, Qilu Hospital, Shandong University, Jinan 250012, 
      Shandong Province, China.
FAU - Cui, Xiao
AU  - Cui X
AD  - Department of Gastroenterology, Qilu Hospital, Shandong University, Jinan 250012, 
      Shandong Province, China.
FAU - Liu, Yang
AU  - Liu Y
AD  - Department of Medicine, Beijing 316 Hospital, Beijing 100093, China.
FAU - Zuo, Xiu-Li
AU  - Zuo XL
AD  - Department of Gastroenterology, Qilu Hospital, Shandong University, Jinan 250012, 
      Shandong Province, China. zuoxiuli_s@163.com.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - World J Gastroenterol
JT  - World journal of gastroenterology
JID - 100883448
RN  - 8T3HQG2ZC4 (Trinitrobenzenesulfonic Acid)
SB  - IM
MH  - Animals
MH  - Clostridium butyricum/*immunology
MH  - Dendritic Cells/*immunology
MH  - Disease Models, Animal
MH  - Humans
MH  - Immunity, Mucosal
MH  - Intestinal Mucosa/cytology/*immunology/microbiology
MH  - Irritable Bowel Syndrome/chemically induced/immunology/*therapy
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Probiotics/*administration & dosage
MH  - Treatment Outcome
MH  - Trinitrobenzenesulfonic Acid/toxicity
PMC - PMC6767978
OTO - NOTNLM
OT  - Clostridium butyricum
OT  - Irritable bowel syndrome
OT  - Lamina propria dendritic cells
OT  - Proinflammatory cytokines
OT  - T cell immunoglobulin and mucin domain-3
COIS- Conflict-of-interest statement: The authors declare no conflicts of interest 
      related to this manuscript or its publication.
EDAT- 2019/10/03 06:00
MHDA- 2020/02/27 06:00
PMCR- 2019/09/28
CRDT- 2019/10/03 06:00
PHST- 2019/07/10 00:00 [received]
PHST- 2019/08/17 00:00 [revised]
PHST- 2019/08/24 00:00 [accepted]
PHST- 2019/10/03 06:00 [entrez]
PHST- 2019/10/03 06:00 [pubmed]
PHST- 2020/02/27 06:00 [medline]
PHST- 2019/09/28 00:00 [pmc-release]
AID - 10.3748/wjg.v25.i36.5469 [doi]
PST - ppublish
SO  - World J Gastroenterol. 2019 Sep 28;25(36):5469-5482. doi: 
      10.3748/wjg.v25.i36.5469.