PMID- 31580709
OWN - NLM
STAT- MEDLINE
DCOM- 20191114
LR  - 20191114
IS  - 1208-6002 (Electronic)
IS  - 0829-8211 (Linking)
VI  - 97
IP  - 5
DP  - 2019 Oct
TI  - Low n-6/n-3 PUFA ratio improves inflammation and myocardial ischemic reperfusion 
      injury.
PG  - 621-629
LID - 10.1139/bcb-2018-0342 [doi]
AB  - This study investigated the potential effect of n-6/n-3 polyunsaturated fatty 
      acids (PUFA) on inflammation and myocardial ischemic reperfusion injury (MIRI) in 
      rats, together with the underlying protective mechanisms, and screen out most 
      effective ratio of n-6/n-3 within limits. The rats with pre-infarct treatment 
      were distributed among 5 groups according to the n-6/n-3 ratio (36:1; 1:1, 5:1, 
      10:1, 50:1); for the post-infarct treatment, the rats were distributed among 6 
      groups, including the control group (36:1) which was subjected to a sham 
      procedure; the model group (36:1); and 4 test groups (n-6/n-3 ratio: 1:1, 5:1, 
      10:1, 50:1). All of the rats were fed a purple perilla seed oil and safflower 
      oil-based fatty emulsion. The serum levels of monocyte chemoattractant protein-1 
      (MCP-1), interleukin (IL)-1beta, IL-6, and tumor necrosis factor (TNF)-alpha were 
      determined using enzyme-linked immunosorbent assay. Staining with triphenyl 
      tetrazolium chloride, hematoxylin and eosin, or Masson's trichrome was performed 
      for histological examination. Cardiomyocyte apoptosis was examined by TUNEL 
      assay. Western blotting was performed to examine the expression levels of 
      apoptosis-related proteins and signaling pathway proteins. Our data indicate that 
      in both the pre-infarct treatment and post-infarct treatment, low ratios of 
      n-6/n-3 PUFAs significantly inhibited the levels of serum inflammatory factors, 
      the infarct size of MIRI rats, number of cardiomyocytes undergoing apoptosis, and 
      the expression levels of caspase-3, Bcl-2, and Bax in the MIRI group. Thus a low 
      ratio of n-6/n-3 PUFAs ameliorates inflammation and myocardial ischemic 
      reperfusion injury.
FAU - Ma, Caiyan
AU  - Ma C
AD  - Cardiovascular Department, Tongde Hospital of Zhejiang Province, Hangzhou, 
      Zhejiang Province, 310012, People's Republic of China.
AD  - Cardiovascular Department, Tongde Hospital of Zhejiang Province, Hangzhou, 
      Zhejiang Province, 310012, People's Republic of China.
FAU - Xu, Zehang
AU  - Xu Z
AD  - Cardiovascular Department, Tongde Hospital of Zhejiang Province, Hangzhou, 
      Zhejiang Province, 310012, People's Republic of China.
AD  - Cardiovascular Department, Tongde Hospital of Zhejiang Province, Hangzhou, 
      Zhejiang Province, 310012, People's Republic of China.
FAU - Lv, Heng
AU  - Lv H
AD  - Cardiovascular Department, Tongde Hospital of Zhejiang Province, Hangzhou, 
      Zhejiang Province, 310012, People's Republic of China.
AD  - Cardiovascular Department, Tongde Hospital of Zhejiang Province, Hangzhou, 
      Zhejiang Province, 310012, People's Republic of China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20191003
PL  - Canada
TA  - Biochem Cell Biol
JT  - Biochemistry and cell biology = Biochimie et biologie cellulaire
JID - 8606068
RN  - 0 (Fatty Acids, Omega-3)
RN  - 0 (Fatty Acids, Omega-6)
SB  - IM
MH  - Animals
MH  - Apoptosis/drug effects
MH  - Dietary Supplements
MH  - Fatty Acids, Omega-3/administration & dosage/metabolism/*pharmacology
MH  - Fatty Acids, Omega-6/administration & dosage/metabolism/*pharmacology
MH  - Inflammation/*drug therapy/metabolism/pathology
MH  - Male
MH  - Myocardial Reperfusion Injury/*drug therapy/metabolism/pathology
MH  - Myocytes, Cardiac/drug effects
MH  - Rats
MH  - Rats, Sprague-Dawley
OTO - NOTNLM
OT  - AGPI n-6/n-3
OT  - DIRM
OT  - MIRI
OT  - inflammation
OT  - n-6/n-3 PUFA
EDAT- 2019/10/04 06:00
MHDA- 2019/11/15 06:00
CRDT- 2019/10/04 06:00
PHST- 2019/10/04 06:00 [pubmed]
PHST- 2019/11/15 06:00 [medline]
PHST- 2019/10/04 06:00 [entrez]
AID - 10.1139/bcb-2018-0342 [doi]
PST - ppublish
SO  - Biochem Cell Biol. 2019 Oct;97(5):621-629. doi: 10.1139/bcb-2018-0342. Epub 2019 
      Oct 3.