PMID- 31582854
OWN - NLM
STAT- MEDLINE
DCOM- 20200211
LR  - 20231213
IS  - 1545-9985 (Electronic)
IS  - 1545-9993 (Print)
IS  - 1545-9985 (Linking)
VI  - 26
IP  - 10
DP  - 2019 Oct
TI  - FACT mediates cohesin function on chromatin.
PG  - 970-979
LID - 10.1038/s41594-019-0307-x [doi]
AB  - Cohesin is a regulator of genome architecture with roles in sister chromatid 
      cohesion and chromosome compaction. The recruitment and mobility of cohesin 
      complexes on DNA is restricted by nucleosomes. Here, we show that the role of 
      cohesin in chromosome organization requires the histone chaperone FACT 
      ('facilitates chromatin transcription') in Saccharomyces cerevisiae. We find that 
      FACT interacts directly with cohesin, and is dynamically required for its 
      localization on chromatin. Depletion of FACT in metaphase cells prevents cohesin 
      accumulation at pericentric regions and causes reduced binding on chromosome 
      arms. Using the Hi-C technique, we show that cohesin-dependent TAD (topological 
      associated domain)-like structures in G1 and metaphase chromosomes are reduced in 
      the absence of FACT. Sister chromatid cohesion is intact in FACT-depleted cells, 
      although chromosome segregation failure is observed. Our data show that FACT 
      contributes to the formation of cohesin-dependent TADs, thus uncovering a new 
      role for this complex in nuclear organization during interphase and mitotic 
      chromosome folding.
FAU - Garcia-Luis, Jonay
AU  - Garcia-Luis J
AD  - Cell Cycle Group, MRC London Institute of Medical Sciences (LMS), London, UK.
FAU - Lazar-Stefanita, Luciana
AU  - Lazar-Stefanita L
AD  - Institut Pasteur, Department of Genomes and Genetics, Unite Regulation Spatiale 
      des Genomes, Paris, France.
FAU - Gutierrez-Escribano, Pilar
AU  - Gutierrez-Escribano P
AD  - Cell Cycle Group, MRC London Institute of Medical Sciences (LMS), London, UK.
FAU - Thierry, Agnes
AU  - Thierry A
AD  - Institut Pasteur, Department of Genomes and Genetics, Unite Regulation Spatiale 
      des Genomes, Paris, France.
FAU - Cournac, Axel
AU  - Cournac A
AD  - Institut Pasteur, Department of Genomes and Genetics, Unite Regulation Spatiale 
      des Genomes, Paris, France.
FAU - Garcia, Alicia
AU  - Garcia A
AD  - Instituto de Biologia Funcional y Genomica (IBFG), CSIC/Universidad de Salamanca, 
      Salamanca, Spain.
FAU - Gonzalez, Sara
AU  - Gonzalez S
AD  - Instituto de Biologia Funcional y Genomica (IBFG), CSIC/Universidad de Salamanca, 
      Salamanca, Spain.
FAU - Sanchez, Mar
AU  - Sanchez M
AD  - Instituto de Biologia Funcional y Genomica (IBFG), CSIC/Universidad de Salamanca, 
      Salamanca, Spain.
FAU - Jarmuz, Adam
AU  - Jarmuz A
AD  - Cell Cycle Group, MRC London Institute of Medical Sciences (LMS), London, UK.
FAU - Montoya, Alex
AU  - Montoya A
AD  - Biological Mass Spectrometry and Proteomics Facility, MRC London Institute of 
      Medical Sciences (LMS), London, UK.
FAU - Dore, Marian
AU  - Dore M
AD  - Bioinformatics Facility, MRC London Institute of Medical Sciences (LMS), London, 
      UK.
FAU - Kramer, Holger
AU  - Kramer H
AD  - Biological Mass Spectrometry and Proteomics Facility, MRC London Institute of 
      Medical Sciences (LMS), London, UK.
FAU - Karimi, Mohammad M
AU  - Karimi MM
AUID- ORCID: 0000-0001-5017-1252
AD  - Bioinformatics Facility, MRC London Institute of Medical Sciences (LMS), London, 
      UK.
FAU - Antequera, Francisco
AU  - Antequera F
AD  - Instituto de Biologia Funcional y Genomica (IBFG), CSIC/Universidad de Salamanca, 
      Salamanca, Spain.
FAU - Koszul, Romain
AU  - Koszul R
AUID- ORCID: 0000-0002-3086-1173
AD  - Institut Pasteur, Department of Genomes and Genetics, Unite Regulation Spatiale 
      des Genomes, Paris, France. romain.koszul@pasteur.fr.
FAU - Aragon, Luis
AU  - Aragon L
AUID- ORCID: 0000-0003-0634-6742
AD  - Cell Cycle Group, MRC London Institute of Medical Sciences (LMS), London, UK. 
      luis.aragon@csc.mrc.ac.uk.
LA  - eng
GR  - Wellcome Trust/United Kingdom
GR  - 100955/Wellcome Trust/United Kingdom
GR  - MC_U120074328/MRC_/Medical Research Council/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20191003
PL  - United States
TA  - Nat Struct Mol Biol
JT  - Nature structural & molecular biology
JID - 101186374
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (Chromatin)
RN  - 0 (Chromosomal Proteins, Non-Histone)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (FACT protein, S cerevisiae)
RN  - 0 (High Mobility Group Proteins)
RN  - 0 (SPT16 protein, S cerevisiae)
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - 0 (Transcriptional Elongation Factors)
RN  - 0 (structural maintenance of chromosome protein 1)
SB  - IM
MH  - Cell Cycle Proteins/*metabolism
MH  - Chromatin/metabolism
MH  - Chromosomal Proteins, Non-Histone/*metabolism
MH  - DNA-Binding Proteins/metabolism
MH  - High Mobility Group Proteins/metabolism
MH  - Protein Interaction Maps
MH  - Saccharomyces cerevisiae/cytology/*metabolism
MH  - Saccharomyces cerevisiae Proteins/*metabolism
MH  - Transcriptional Elongation Factors/*metabolism
MH  - Cohesins
PMC - PMC6779571
MID - EMS84179
COIS- Competing interests The authors declare no competing interests.
EDAT- 2019/10/05 06:00
MHDA- 2020/02/12 06:00
PMCR- 2020/04/03
CRDT- 2019/10/05 06:00
PHST- 2019/06/18 00:00 [received]
PHST- 2019/08/21 00:00 [accepted]
PHST- 2019/10/05 06:00 [entrez]
PHST- 2019/10/05 06:00 [pubmed]
PHST- 2020/02/12 06:00 [medline]
PHST- 2020/04/03 00:00 [pmc-release]
AID - 10.1038/s41594-019-0307-x [pii]
AID - 10.1038/s41594-019-0307-x [doi]
PST - ppublish
SO  - Nat Struct Mol Biol. 2019 Oct;26(10):970-979. doi: 10.1038/s41594-019-0307-x. 
      Epub 2019 Oct 3.